Micromonospora spongicola sp. nov., an actinomycete isolated from a marine sponge in the Gulf of Thailand

Abstract

An actinomycete strain, S3-1(T), was isolated from marine sponge sample collected from the Gulf of Thailand. The strain is aerobic, Gram-positive and produced single spores at the tip of the substrate mycelium. Strain S3-1(T) contained meso-diaminopimelic acid in the peptidoglycan, whole-cell sugars were arabinose, galactose, glucose, rhamnose, ribose and xylose. The polar lipid profile of strain S3-1(T) consisted of phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, phosphoglycolipid and unknown polar lipids. Morphological and chemotaxonomic characteristics of the strain were identified as a member of the genus Micromonospora. Phylogenetic analysis based on 16S rRNA gene sequence analysis of the strain showed similarity to Micromonospora nigra DSM 43818(T) (98.8%), Micromonospora yangpuensis FXJ6.011(T) (98.7%) and Micromonospora narathiwatensis BTG4-1(T) (98.6%). The DNA G+C content was 72.7 mol%. The phenotypic characteristics and DNA-DNA relatedness values supported that the classification of this strain as a novel species in the genus Micromonospora, for which the name Micromonospora spongicola sp. nov. (type strain S3-1(T) =BCC 45595(T)=NBRC 108779(T)) is proposed.

Recent progress on the development of antibiotics from the genus Micromonospora

“…This remained the only known strain among the actinomycetes bearing the generic name Micromonospora until Jensen in 1930 described a large number of soil micro-organisms which corresponded with Ørskov's description [19]. At the time of writing, the genus consisted of 61 validly named species (http://www.bacterio.net) [20], in addition to the recently described species, like: M. spongicola [21], M. jinlongensis [22], M. zeae [23], M. maoerensis [24], M. endophytica [25], M. palomenae [26], M. harpali [26], M. oryzae [27], M. vulcania [28], M. fluostatini [29], M. nickelidurans [30], M.…”

Biochemical Screening of Marine Micromonospora Marina Kpms1 (Mh036351) and Its Antibacterial Activity Against Multidrug Resistant Bacteria

“…Currently, Micromonospora sp. was isolated from marine sponge, marine water, and it sediments [4]. Not only soil region, the marine water and its sediments also harbor microbial rich environment which have abundant microbial communities ranging from 10 6 to 10 9 CFU/g [5].…”

Micromonospora fluostatini sp. nov., isolated from marine sediment

“…The genus Micromonospora , accommodating aerobic, Gram-stain-positive, filamentous bacteria that form single non-motile spores on substrate mycelia, was initially described by Ørskov (1923). Although many species of the genus Micromonospora have been isolated from diverse soils collected from different regions (Genilloud, 2012), some have been isolated from root nodules (Trujillo et al , 2006), sea sand (Tanasupawat et al , 2010) and marine sponge (Supong et al , 2013). Since discovery of the first antibiotics, more than 740 bioactive metabolites, such as gentamicins, neomycin, rifamycins, sisomicin, erythromycin B and fortimicins, have been isolated from the family Micromonosporaceae and have been used for clinical treatment (Bérdy, 2005; Wagman & Weinstein, 1980).…”