Micromixer Based Preparation of Functionalized Liposomes and Targeting Drug Delivery

Jia, Xiangqian; Wang, Weizhi; Han, Qiuju; Wang, Zihua; Jia, Yunhong; Hu, Zhiyuan

doi:10.1021/acsmedchemlett.6b00028

Cited by 19 publications

(12 citation statements)

References 30 publications

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“…Functionalization of liposomes with a breast cancer targeting peptide (H6, YLFFVFER) as a targeting nanocarrier system ( Figure 7 ) shows high efficiency since antitumor drugs were successfully delivered into human epidermal growth factor receptor 2 (HER2) positive breast cancer cells in both in vivo and ex vivo models [ 217 ]. Additionally, PEGylated liposomes modified with OX26 and chlorotoxin CTX were shown to promote cell transfection and improve the transport of plasmid DNA across the blood–brain barrier which further fights the brain glioma cells in vitro and in vivo [ 218 ].…”

Section: Types Of Nanocarriersmentioning

confidence: 99%

See 1 more Smart Citation

Surface Modified Multifunctional and Stimuli Responsive Nanoparticles for Drug Targeting: Current Status and Uses

Sıafaka

Okur

Karavas

et al. 2016

IJMS

156

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Nanocarriers, due to their unique features, are of increased interest among researchers working with pharmaceutical formulations. Polymeric nanoparticles and nanocapsules, involving non-toxic biodegradable polymers, liposomes, solid lipid nanoparticles, and inorganic–organic nanomaterials, are among the most used carriers for drugs for a broad spectrum of targeted diseases. In fact, oral, injectable, transdermal-dermal and ocular formulations mainly consist of the aforementioned nanomaterials demonstrating promising characteristics such as long circulation, specific targeting, high drug loading capacity, enhanced intracellular penetration, and so on. Over the last decade, huge advances in the development of novel, safer and less toxic nanocarriers with amended properties have been made. In addition, multifunctional nanocarriers combining chemical substances, vitamins and peptides via coupling chemistry, inorganic particles coated by biocompatible materials seem to play a key role considering that functionalization can enhance characteristics such as biocompatibility, targetability, environmental friendliness, and intracellular penetration while also have limited side effects. This review aims to summarize the “state of the art” of drug delivery carriers in nanosize, paying attention to their surface functionalization with ligands and other small or polymeric compounds so as to upgrade active and passive targeting, different release patterns as well as cell targeting and stimuli responsibility. Lastly, future aspects and potential uses of nanoparticulated drug systems are outlined.

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Section: Types Of Nanocarriersmentioning

confidence: 99%

“… Schematic presentation of functionalized liposomes (pink sphere) with breast cancer targeting peptide (purple structure) and confocal images of liposomes (green spheres) in fluorescein isothiocyanate [ 217 ]. …”

Section: Figurementioning

confidence: 99%

Surface Modified Multifunctional and Stimuli Responsive Nanoparticles for Drug Targeting: Current Status and Uses

Sıafaka

Okur

Karavas

et al. 2016

IJMS

156

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“…To enhance the accuracy of drug delivery to tumors, controlled drug-delivery systems have been functionalized with a variety of tumor-specific targeting ligands-such as aptamers, [28][29][30] peptides, 1,[30][31][32] antibodies and growth factors 33 -to selectively target molecules and receptors that are overexpressed on the surface of tumor cells. Aptamers, single-stranded DNA or RNA oligonucleotides, 34,35 are a novel type of targeting agent that offer several advantages over other tumor-specific ligands.…”

Section: Introductionmentioning

confidence: 99%

<p>A dual-functional HER2 aptamer-conjugated, pH-activated mesoporous silica nanocarrier-based drug delivery system provides in vitro synergistic cytotoxicity in HER2-positive breast cancer cells</p>

Shen

Liu

et al. 2019

IJN

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Purpose: As well as functioning as a ligand that is selectively internalized by cells overexpressing human epidermal growth factor receptor-2 (HER2), HApt can exert cytotoxic effects by inducing cross-linking and subsequent translocation of HER2 to cytoplasmic vesicles, such downregulation of HER2 inhibits cell proliferation and induces apoptosis. We aimed to exploit the potential of HApt as both a targeting agent and antagonist to maximize the efficacy of mesoporous silica nanoparticle (MSN)-based drug release systems for HER2-positive breast cancer. Materials and methods: We fabricated novel HApt aptamer-functionalized pH-sensitive βcyclodextrin (β-CD)-capped doxorubicin (DOX)-loaded mesoporous silica nanoparticles (termed MSN-BM/CD-HApt@DOX) for targeted delivery and selective targeting of HER2-positive cells. MSN-functionalized benzimidazole (MSN-BM) was used to load and achieve pH stimuliresponsive release of the chemotherapeutic agent doxorubicin (DOX). β-cyclodextrin was introduced as a gatekeeper for encapsulated DOX and HApt as a selective HER2-targeting moiety and biotherapeutic agent. Results: Physical and chemical characterizations (FT-IR, XRD, TEM and BET) confirmed successful construction of MSN-BM/CD-HApt@DOX nanoparticles. In vitro release assays verified pH-sensitive DOX release. MSN-BM/CD-HApt@DOX (relative DOX concentration, 3.6 μg/mL) underwent HER2-mediated endocytosis and was more cytotoxic to HER2-positive SKBR3 cells than HER2-negative MCF7 cells. MSN-BM/CD-HApt@DOX also exhibited better uptake and stronger growth inhibition in SKBR3 cells than the control MSN-BM/CD-NCApt@DOX functionalized with a scrambled nucleotide sequence on CD. Overall, intracellular delivery of DOX and the biotherapeutic agent HApt resulted in synergistic cytotoxic effects in HER2-positive cancer cells in comparison to either DOX or HApt alone. Conclusion: MSN-BM/CD-HApt@DOX enables HER2-mediated targeting and biotherapeutic effects as well as pH-responsive DOX drug release, resulting in synergistic cytotoxic effects in HER2-overexpressing cells in vitro. This novel nanocarrier could potentially enable specific targeting to improve the efficacy of chemotherapy for HER2-positive cancer.

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“…Liposomes are small, nano-sized vesicles with an inner aqueous core embraced by one or more lipid membranes, usually consisting of phospholipids. They have been widely studied for drug delivery during the last decades, both for parenteral administration, e.g., targeting to tumors or reduction of toxic side effects [1][2][3], and for topical administration to skin and mucosal membranes [4][5][6]. The great advantage of liposomes is their ability to encapsulate both hydrophilic and hydrophobic drugs.…”

Section: Introductionmentioning

confidence: 99%

Liposomes coated with hydrophobically modified hydroxyethyl cellulose: Influence of hydrophobic chain length and degree of modification

Smistad

Nyström

Zhu

et al. 2017

Colloids and Surfaces B: Biointerfaces

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Nanoparticulate systems with an uncharged hydrophilic surface may have a great potential in mucosal drug delivery. In the present study liposomes were coated with hydrophobically modified hydroxyethyl cellulose (HM-HEC) to create a sterically stabilized liposomal system with an uncharged surface. The aim was to clarify the influence of the amount of hydrophobic modification of HEC and the length of the hydrophobic moiety, on the stability of the system and on the release properties. HM-HEC with different degrees of hydrophobic modification (1 and 2 mole%) and hydrophobic groups with different chain lengths (C8, C12, C16) were included in the study, as well as fluid phase and gel phase liposomes. Both types of liposomes were successfully coated with HM-HEC containing 1 mole% of hydrophobic groups, while 2 mole% did not work for the intended pharmaceutical applications. The polymer coated gel phase liposomes were stable (size, zeta potential, leakage) for 24 weeks at 4 °C, with no differences between the C8 and C16 HM-HEC coating. For the fluid phase liposomes a size increase was observed after 24 weeks at 4 °C for all formulations; the C8 HM-HEC coated liposomes increased the most. No differences in the leakage during storage at 4 °C or in the release at 35 °C, were observed between the fluid phase formulations. To conclude; HM-HEC with a shorter hydrophobic chain length resulted in a less stable product for the fluid phase liposomes, while no influence of the chain length was observed for the gel phase liposomes (1 mole% HM).

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Micromixer Based Preparation of Functionalized Liposomes and Targeting Drug Delivery

Cited by 19 publications

References 30 publications

Surface Modified Multifunctional and Stimuli Responsive Nanoparticles for Drug Targeting: Current Status and Uses

Surface Modified Multifunctional and Stimuli Responsive Nanoparticles for Drug Targeting: Current Status and Uses

<p>A dual-functional HER2 aptamer-conjugated, pH-activated mesoporous silica nanocarrier-based drug delivery system provides in vitro synergistic cytotoxicity in HER2-positive breast cancer cells</p>

Liposomes coated with hydrophobically modified hydroxyethyl cellulose: Influence of hydrophobic chain length and degree of modification

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