Micrografting and ex vitro grafting for somatic embryo rescue and plant recovery in avocado (Persea americana)

Raharjo, Simon H. T.; Litz, R. E.

doi:10.1007/s11240-004-5486-3

Cited by 35 publications

(31 citation statements)

References 21 publications

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“…Advantages of grafting shoots are discussed in more detail by Lane et al (2003). Once grafted, scion growth occurs after the union of cambium tissue between the scion and rootstock, or graft union, is established (Raharjo and Litz 2005). Acclimatization of the scion to greenhouse conditions is achieved as it emerges from the Parafilm covering (Lane et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Shoot regeneration from organogenic callus of sweet cherry (Prunus avium L.)

Feeney¹,

Bhagwat

Mitchell

et al. 2007

Plant Cell Tiss Organ Cult

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A complete method to regenerate adventitious shoots and to produce field-ready trees from three commercial cultivars of sweet cherry (Prunus avium L.) is described. The effects of explant types, pre-treatments, basal media, and phloroglucinol on cultivars Bing, Sweetheart, and Lapins were investigated. Callus developed on four explant types: apical shoot tips isolated from orchard trees; and punctured shoot tips, stem sections, and shoot bases of in vitro shoot cultures. Callus formed on Bing (5%), Sweetheart (8%), and Lapins (20%) shoot tips from orchard trees after 4 months on Murashige and Skoog medium (MS) at half-strength with 3 mM benzylaminopurine (BA). In vitro-derived explants formed callus after 3 months on Woody Plant Medium with 3 mM BA (W3B): punctured shoot tips (Sweetheart and Lapins 67%), stem sections (Sweetheart 31%, Lapins 27%), and shoot bases (Sweetheart 10%, Lapins 17%). Pre-treatment of shoot cultures on MS with 3 mM BA and 1 mM phloroglucinol increased callus formation three-fold on shoot base explants. Callus was separated from parental explants and maintained on MS with 3 mM BA. Shooting was induced by transferring callus to W3B. At 2 weeks, shoot development approached 100%. By 4 weeks, 7-17 shoots had formed on each explant. Callus was maintained for 1.5 years with no decrease in shoot production. Shoots were grafted onto Mazzard (P. avium) rootstocks with 54% (Sweetheart), 57% (Lapins), and 21% (Bing) success after 5 weeks.

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Section: Discussionmentioning

confidence: 99%

Shoot regeneration from organogenic callus of sweet cherry (Prunus avium L.)

Feeney¹,

Bhagwat

Mitchell

et al. 2007

Plant Cell Tiss Organ Cult

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“…Paz & Pasqual (1998) afirmam que é comum, na microenxertia in vitro em citros, taxas de pegamento que variam de 2%, quando se utilizam ápices com 0,05 mm de comprimento, a 50%, quando se utilizam ápices com seis primórdios foliares. Raharjo & Litz (2005) testaram a microenxertia em abacateiro (Persea americana), em condição in vitro e ex vitro, e observaram que os resultados referentes ao pegamento foram próximos e que as brotações obtidas na microenxertia ex vitro apresentaram maior desenvolvimento do que as obtidas na microenxertia in vitro.…”

Section: Resultsunclassified

Microenxertia ex vitro para eliminação do vírus CABMV em maracujá-azedo

Ribeiro

Peixoto

Andrade

et al. 2008

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar diferentes meios de cultura, utilizados sobre o ponto da enxertia, na microenxertia ex vitro para a eliminação do Cowpea aphid-borne mosaic virus (CABMV), em plantas de maracujá-azedo (Passiflora edulis Sims f. flavicarpa Deg.). Ápices caulinares, provenientes de plantas infectadas, foram microenxertados em plântulas obtidas pela germinação de sementes em substrato comercial esterilizado. Foram conduzidos experimentos com a microenxertia realizada no hipocótilo e no epicótilo, e foram utilizados cinco meios de cultura, que diferiam na concentração de fitorreguladores, aplicados no local da enxertia. O índice médio de microenxertos com folha expandida foi de 27,22 e 32,22%, quando a microenxertia foi realizada no hipocótilo e no epicótilo, respectivamente. Na microenxertia realizada no hipocótilo, não houve efeito da aplicação de meios de cultura. Na microenxertia realizada no epicótilo, o meio MS acrescido de 0,1 mg L -1de AIB e 1 mg L -1 de BAP proporcionou 53,3% de microenxertos com folha expandida, número superior aos demais tratamentos e maior desenvolvimento das brotações. A indexação realizada pelo teste ELISA indireto, 80 a 100 dias após a microenxertia, mostrou que 93% das plantas testadas não apresentavam vírus detectável.Termos para indexação: Cowpea aphid-borne mosaic virus, Passiflora edulis, endurecimento dos frutos, local de enxertia. Ex vitro micrografting aiming the CABMV virus elimination in passion fruit plantAbstract -The objective of this work was to evaluate the effect of different culture media, used over grafting spot, in ex vitro micrografting, in order to eliminate the Cowpea aphid-borne mosaic virus (CABMV) in sour passion fruit plants (Passiflora edulis Sims f. flavicarpa Deg.). Cauline apexes, originated from infected plants, were micrografted in seedlings obtained by the germination of seeds in commercial sterilized substrate. Experiments were conducted with the micrografting performed on the hypocotyl and epicotyl using five culture media, which differed on their concentration of phytoregulators applied on the grafting spot. The average rate of micrografting showing expanded leaf was 27.22 and 32.22%, when micrografting was performed on hypocotyl and epicotyl, respectively. In the micrografting performed on the hypocotyl, there was no effect of the culture media applied. In the micrografting performed on epicotyl, the MS medium supplemented with 0.1 mg L -1 3-indolbutiric acid (IBA) and 1 mg L -1 6-benzylaminopurine (BAP) resulted in 53.3% of micrografting showing expanded leaf, a number superior to the other treatments, and in a larger development of shoots. The indexing performed by the indirect ELISA test, within 80 to 100 days after micrografting, showed that 93% of the plants tested presented no detectable virus.

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“…Besides, pistachio in vivo grown scions grafted onto in vitro seedlings failed (Can et al, 2006), while ex vitro grafting of shoots that have been produced in vitro, as here, is an uncommon technique used mainly to rescue transgenic rootless shoots, as in grapefruit or sunflower (Yang et al, 2000;Weber et al, 2003), or somatic embryos in avocado (Raharjo and Litz, 2005). Here, grafting in vitro growing shoot tips onto terebinth seedlings has resulted in a great advantage due, not only to a relatively high graft success, but to shorten time and facilitate pistachio propagation.…”

Section: Mul Plica On Ratesmentioning

confidence: 99%

Propagation of pistachio applying in vitro culture techniques

Marín¹,

García²,

Lorente³

et al. 2017

Acta Hortic.

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Pistachio grafting often shows a high degree of uncertainty, with variable and inconsistent results with commonly used rootstocks, producing irregular orchards and frequent regrafting. Besides, the size of the buds of pistachio cultivars far exceeds that of the juvenile rootstock terebinth (Pistacia terebinthus L.), widely used in Spain, making pistachio grafting more difficult. Here, we have applied in vitro techniques to obtain reduced-size cultivars that match to the rootstock size. By reducing bud/scion of pistachio cultivars we can thus graft them on terebinth seedlings few weeks after germination. The female cultivars 'Larnaka', 'Kerman' and 'Sirora', as well as the male cultivar 'Peters' and the selection AD15 have been successfully cultivated in vitro. Grafting was performed on juvenile terebinth plants grown in small containers using a) micropropagated and acclimatized pistachio plants or b) in vitro grown shoot-tips as scion source. We have got up to 68% successful grafts with scions derived from in vitro techniques. Scions resumed growth few days after grafting, and time required to obtain new plants was significantly shortened.

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Micrografting and ex vitro grafting for somatic embryo rescue and plant recovery in avocado (Persea americana)

Cited by 35 publications

References 21 publications

Shoot regeneration from organogenic callus of sweet cherry (Prunus avium L.)

Shoot regeneration from organogenic callus of sweet cherry (Prunus avium L.)

Microenxertia ex vitro para eliminação do vírus CABMV em maracujá-azedo

Propagation of pistachio applying in vitro culture techniques

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