Shoot regeneration from organogenic callus of sweet cherry (Prunus avium L.)

Abstract

A complete method to regenerate adventitious shoots and to produce field-ready trees from three commercial cultivars of sweet cherry (Prunus avium L.) is described. The effects of explant types, pre-treatments, basal media, and phloroglucinol on cultivars Bing, Sweetheart, and Lapins were investigated. Callus developed on four explant types: apical shoot tips isolated from orchard trees; and punctured shoot tips, stem sections, and shoot bases of in vitro shoot cultures. Callus formed on Bing (5%), Sweetheart (8%), and Lapins (20%) shoot tips from orchard trees after 4 months on Murashige and Skoog medium (MS) at half-strength with 3 mM benzylaminopurine (BA). In vitro-derived explants formed callus after 3 months on Woody Plant Medium with 3 mM BA (W3B): punctured shoot tips (Sweetheart and Lapins 67%), stem sections (Sweetheart 31%, Lapins 27%), and shoot bases (Sweetheart 10%, Lapins 17%). Pre-treatment of shoot cultures on MS with 3 mM BA and 1 mM phloroglucinol increased callus formation three-fold on shoot base explants. Callus was separated from parental explants and maintained on MS with 3 mM BA. Shooting was induced by transferring callus to W3B. At 2 weeks, shoot development approached 100%. By 4 weeks, 7-17 shoots had formed on each explant. Callus was maintained for 1.5 years with no decrease in shoot production. Shoots were grafted onto Mazzard (P. avium) rootstocks with 54% (Sweetheart), 57% (Lapins), and 21% (Bing) success after 5 weeks.

Micropropagtion of Terminalia bellerica from nodal explants of mature tree and assessment of genetic fidelity using ISSR and RAPD markers

“…Therefore, the shoots obtained from nodal explants were elongated by repeated subculture at 2-week interval on medium containing BA (4.4 μM) and PG (3.9 μM). Similar effect of PG has been reported by Sarkar and Naik (2000), Feeney et al (2007) and Jain et al (2011).…”

Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective

“…Regeneration and transformation protocols for P. cerasus (Mante et al, 1989;Tang et al, 2002;Sink, 2005, 2006) and P. avium (Hammatt and Grant, 1998;Tang et al, 2002;Matt and Jehle, 2005;Feeney et al, 2007) were based on adventitious regeneration using leaves and internodes from plants grown in vitro and hypocotyl slices.…”

In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks

“…1) and the first day of callus induction. The friable callus tissues were cut into For most purposes, in vitro callus establishment is important as an intermediate step in biotechnology and development of these biotechnological tools from mature tissues is important for the improvement of desirable commercial cultivars (Druart, 1980(Druart, , 1999Hammatt and Grant, 1998;Gentile et al, 2002;Bhagwat and Lane, 2004;Fajerska, 2006, Feeney et al, 2007. Callus is an amorphous and dedifferentiated, disorganized and non-homogeneous tissue produced by a plant as a response to insect or microorganism attack or stressful situations (George, 1993).…”

“…have been used for somatic organogenesis and embryogenesis, protoplast fusion, and hybridization (Druart, 1980(Druart, , 1999Jones et al, 1984;Garin et al, 1997;Tang et al, 2000). It has also been exploited in agricultural practices, horticulture, forestry and industry in order to achieve mass propagation of virus free plants (Neil and Neil, 2000;Akita et al, 2006;Feeney et al, 2007;Kalinina and Brown, 2007;Ružić et al, 2010). Many of these areas include working within the in vitro culture of the callus and one of the direction of the in vitro culture development are grafting compatibility of the rootstock and scion (Errea et al, 1994;2001;Nito et al, 2005;Todić et al, 2005).…”