Microglia in Cell Culture and in Transplantation Therapy for Central Nervous System Disease

Organic electronics for precise delivery of neurotransmitters to modulate mammalian sensory function

“…For in vitro testing, primary murine astrocyte cultures were prepared and maintained as previously described 35 , then loaded with the Ca 2+ -sensitive dye FURA-2 AM. The encapsulated device was evaluated by delivering Glu and observing the subsequent fluxes in intracellular [Ca 2+ ] via ratiometric time-lapse fluorescence microscopy.…”

Migration and differentiation of neural precursor cells can be directed by microglia

“…Cell cultures were kept at 37°C in 10% CO 2 and 90% relative humidity. Primary microglia (PM) was prepared from embryonic day (E) 16 to E17 C57͞BL mice, with some modifications, as described (26). In summary, isolated tissue was mechanically dissociated between the frosted ends of two glass slides, filtered trough a 70-m nylon mesh (Falcon), and washed in culture medium (DMEM 10% FCS͞0.45% glucose) before seeded in poly(L)-lysine-coated flasks.…”

Behavior of in vitro cultured ameboid microglial cells migrating on Müller cell end‐feet in the quail embryo retina

“…To date, most methods of microglial cell culture have obtained primary mixed cell cultures after tissue dissociation, generally from neonatal rodent brain or retina (Dobrenis, 1998;Giulian and Baker, 1986;Roque and Caldwell, 1993;Santambrogio et al, 2001;Wang et al, 2005). These primary mixed cell cultures are maintained for 7-14 days and microglia are then isolated by shaking because of their weak adherence to the cell substrate.…”

“…Most methods to obtain microglial cell enriched cultures are based on differences in in vitro adhesion properties among cell types of the nervous tissue (Dobrenis, 1998;Giulian and Baker, 1986;Nakajima et al, 1989;Suzumura et al, 1987). These techniques take advantage of the weak adherence of microglial cells to the culture substrate in order to isolate them from primary mixed brain cell cultures and have also been used to isolate and culture microglia from the retina (Matsubara et al, 1999;Roque and Caldwell, 1993;Wang et al, 2005).…”