Microfungal diversity of Juncus trifidus L. and Salix herbacea L. at isolated locations in the Sudetes and Carpathian Mountains

Jacewski, Brayan; Urbaniak, Jacek; Kwiatkowski, Paweł; Pusz, W.

doi:10.5586/am.1118

Cited by 4 publications

(5 citation statements)

References 20 publications

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“…Several authors have reported advances in the development of tissue cultures for these species [12][13][14][15] , including studies that evidenced the differential responses to tissue cultures of different populations of C. quitensis 16,17 . However, the in vitro establishment of these species has been hampered due to endophytic microorganisms [18][19][20][21][22] that develop in the nutritive media once the explants are placed in them. This causes direct and indirect tissue mortality, necrosis, reduced proliferation of roots and shoots and, in some cases, the death of tissues or plants in their entirety 23 .…”

mentioning

confidence: 99%

Advances of native and non-native Antarctic species to in vitro conservation: improvement of disinfection protocols

Cuba‐Díaz

Rivera-Mora

Navarrete

et al. 2020

Sci Rep

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Plants that inhabit Antarctica have raised scientific interest due to their resilience to climate change, abiotic tolerance mechanisms and potential biological applications. In vitro propagation is useful for conservation, genetic material availability of these species and avoiding mass collection in their habitat. In vitro culture protocols for the native plants Colobanthus quitensis and Deschampsia antarctica and the non-native Juncus bufonius have been affected by endophytic microorganisms that proliferate when introduced to tissue cultures. This study evaluated the microbicidal and phytotoxic effect of calcium hypochlorite (ca(clo) 2), silver nitrate (AgNO 3) and silver nanoparticles (AgNPs), and their use at different concentrations for different time periods. The Ca(ClO) 2 at 100 mg mL −1 showed the best microbial contamination control in D. antarctica (applied for 20 min) and for the three C. quitensis populations (applied for 15 min). In J. bufonius, AgNO 3 at 10 mg mL −1 for 10 min reduced the microbial growth, but oxidative damage was generated. AgNPs did not prevent contamination or have adverse effects on tissues. Survival plantlets from each treatment, population or species were effectively introduced to the tissue culture and their propagation was successful. these results constitute a fundamental advance for the introduction, propagation and conservation of Antarctic species and their use in scientific research. Global loss of biodiversity has increased considerably in recent years, which has made implementing conservation strategies essential for preserving and propagating plant genetic resources in each geographical region 1-3. One method of conservation is through the use of germplasm banks, or facilities and centers created to conserve genetic resources under favorable conditions to prolong their survival; the final objective is the ex-situ conservation of specific genetic diversity. They are a source of material that enables many possibilities for a species of interest, from developing new cultivable varieties, biological technologies, or to establishing ecological restoration plans 4. The Antarctic continent is an extreme ecosystem due to its adverse environmental conditions. Its organisms are exposed to restricted availability of water and nutrients, very low temperatures, frequent freezing and thawing cycles, prolonged periods of darkness in the winter and exposure to high UV radiation levels during summer. These environmental characteristics are inhospitable to most organisms that live in temperate areas 5. In addition, the increase in human activity in the region, and the confirmation that Antarctica is one of the critical points of global warming, make it a continent of highest priority for the conservation and development of multinational and multidisciplinary scientific challenges 6,7. There are only two native flowering plants that have been able to colonize some ice-free areas in the Antarctic Peninsula: Deschampsia antarctica Desv. (Poaceae) and Colobanthus quitensis (Kunth.) Bartl....

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confidence: 99%

Advances of native and non-native Antarctic species to in vitro conservation: improvement of disinfection protocols

Cuba‐Díaz

Rivera-Mora

Navarrete

et al. 2020

Sci Rep

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“…2. Fungi noted on stems and roots of Juncus trifidus L.; microfungi were noted on the stems (S) and roots (R) of J. trifidus according to the data of 1999), Chlebicki (1990;2009), Scheuer, Chlebicki (1997), Sukova (2004), Sukova, Chlebicki (2004, Owczarek-Kościelniak, Chlebicki, Sterfingler (2016), Jacewski et al (2019)…”

Section: Conflict Of Interestmentioning

confidence: 99%

“…in the Eastern Sudetes. Chlebicki (2009) and Jacewski et al (2019) cited available information about the occurrence of fungal species noted so far on the stems, leaves and inflorescences of this host plant. DSE (dark septate endophytes) symbionts are root-inhabiting fungi that can act as parasites, mutualists or be neutral (Jumpponen, 2001).…”

Section: Introductionmentioning

confidence: 99%

Fungal endophytes of stems and roots of Juncus trifidus ssp. trifidus from Poland

Chlebicki

2023

AUPCStudiaNaturae

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Paraconiothyrium, Aureobasidium pullulans, Clonostachys rosea, Oidiodendron griseum, Penicillium miczynskii, Alternaria chartarum, Trichoderma viridescens, Umbelopsis autotrophica and Hyaloscypha finlandica have been isolated from the roots of the highland rush (Juncus trifidus L.) from Babia Gora Massif and the Tatra Mts. in the Western Carpathians. Only the Paraconiothyrium sp. was found both in roots and stems and thus can possibly be transferred from the roots to the leaves (or in opposite direction).

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“…Generally, the occurrence of this genus may indicate well-developed decomposition on damaged plant tissues or in soil [35][36][37][38][39]. Even though this group of fungi is classified as common decomposers, individual representatives of this genus are frequently noticed among major fractions of the phylloplane, spermosphere and endophytic mycobiota [40][41][42][43][44].…”

Section: Object Of the Studymentioning

confidence: 99%

Bulletin of the Polish Academy of Sciences: Technical Sciences

Podwin,

Janisz,

Patejuk

et al. 2021

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This paper presents material and technological studies on lab-on-chip (LOC) devices as a first step towards biocompatible and reliable research on microscopic fungi and soil organisms on a microscale. This approach is intended to respond to the growing need for environmental control and protection, by means of modern, miniaturized, portable and dependable microfluidics instrumentation. The authors have presented herein long-term, successful cultivation of different fungi representatives (with emphasis put on Cladosporium macrocarpum) in specially fabricated all-glass LOCs. Notable differences were noted in the development of these creatures on polymer, polydimethylosiloxane (PDMS) cultivation substrates, revealing the uncommon morphological character of the fungi mycelium. The utility of all-glass LOCs was verified for other fungi representatives as well -Fusarium culmorum and Pencilium expansum, showing technical correspondence and biocompatibility of the devices. On that basis, other future applications of the solution are possible, covering, e.g. investigation of additional, environmentally relevant fungi species. Further development of the LOC instrumentation is also taken into consideration, which could be used for cultivation of other soil organisms and study of their mutual relationships within the integrated microfluidic device.

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Microfungal diversity of Juncus trifidus L. and Salix herbacea L. at isolated locations in the Sudetes and Carpathian Mountains

Cited by 4 publications

References 20 publications

Advances of native and non-native Antarctic species to in vitro conservation: improvement of disinfection protocols

Advances of native and non-native Antarctic species to in vitro conservation: improvement of disinfection protocols

Fungal endophytes of stems and roots of Juncus trifidus ssp. trifidus from Poland

Bulletin of the Polish Academy of Sciences: Technical Sciences

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