Microfluidics: reframing biological enquiry

Duncombe, Todd A.; Tentori, Augusto M.; Herr, Amy E.

doi:10.1038/nrm4041

Cited by 278 publications

(207 citation statements)

References 148 publications

(202 reference statements)

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“…Furthermore, a proportion of the allergic population suffers from more than one allergy, due to cross-reactivity with similarly structured allergens, so it is attractive to test more than one allergen at a time [96–99]. An individual who suffers from multiple allergies should be able to test for all of them using a singular device.…”

Section: Background On Food Allergensmentioning

confidence: 99%

Consumer-friendly food allergen detection: moving towards smartphone-based immunoassays

2018

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In this critical review, we provide a comprehensive overview of immunochemical food allergen assays and detectors in the context of their user-friendliness, through their connection to smartphones. Smartphone-based analysis is centered around citizen science, putting analysis into the hands of the consumer. Food allergies represent a significant worldwide health concern and consumers should be able to analyze their foods, whenever and wherever they are, for allergen presence. Owing to the need for a scientific background, traditional laboratory-based detection methods are generally unsuitable for the consumer. Therefore, it is important to develop simple, safe, and rapid assays that can be linked with smartphones as detectors to improve user accessibility. Smartphones make excellent detection systems because of their cameras, embedded flash functions, portability, connectivity, and affordability. Therefore, this review has summarized traditional laboratory-based methods for food allergen detection such as enzyme-linked-immunosorbent assay, flow cytometry, and surface plasmon resonance, and the potential to modernize these methods by interfacing them with a smartphone readout system, based on the aforementioned smartphone characteristics. This is the first review focusing on smartphone-based food-allergen detection methods designed with the intention of being consumer-friendly. Graphical abstractA smartphone-based food allergen detection system in three easy steps (1) sample preparation, (2) allergen detection on a smartphone using antibodies, which then transmits the data wirelessly, (3) analytical results sent straight to smartphone Electronic supplementary materialThe online version of this article (10.1007/s00216-018-0989-7) contains supplementary material, which is available to authorized users.

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Section: Background On Food Allergensmentioning

confidence: 99%

Consumer-friendly food allergen detection: moving towards smartphone-based immunoassays

2018

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“…Fast growth of microfluidic technology has made its way to connect with all main areas of cell biology research (Duncombe et al 2015). It has been increasingly enabling sophisticated experiments in miniaturized devices, and many microfluidics-based cell functional assays have been developed (Duncombe et al 2015). However, a bottleneck that hinders the adoption of microfluidic cell functional assays in life science research and medical applications remains due to knowledge gap and the facility and skill requirement for effective use of microfluidic assays.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to chemotaxis assay as described in this study, we envision that the MS 2 technology can be developed to enable a range of cell functional assays such as cell viability assay, apoptosis assays, cell proliferation assay, cell differentiation assay, cell invasion assay, wound healing assays, cell killing assay, live cell signaling assays, and cell-based organ-on-chip imaging and monitoring. Fast growth of microfluidic technology has made its way to connect with all main areas of cell biology research (Duncombe et al 2015). It has been increasingly enabling sophisticated experiments in miniaturized devices, and many microfluidics-based cell functional assays have been developed (Duncombe et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Mkit: A cell migration assay based on microfluidic device and smartphone

Yang

Peretz‐Soroka

et al. 2018

Biosensors and Bioelectronics

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Mobile sensing based on the integration of microfluidic device and smartphone, so-called MS 2 technology, has enabled many applications over recent years, and continues to stimulate growing interest in both research communities and industries. In particular, it has been envisioned that MS 2 technology can be developed for various cell functional assays to enable basic research and clinical applications. Toward this direction, in this paper, we describe the development of a MS 2 -based cell functional assay for testing cell migration (the M kit ). The system is constructed as an integrated test kit, which includes microfluidic chips, a smartphone-based imaging platform, the phone apps for image capturing and data analysis, and a set of reagent and accessories for performing the cell migration assay. We demonstrated that the M kit can effectively measure purified neutrophil and cancer cell chemotaxis. Furthermore, neutrophil chemotaxis can be tested from a drop of whole blood using the M kit with red blood cell (RBC) lysis. The effects of chemoattractant dose and gradient profile on neutrophil chemotaxis were also tested using the M kit . In addition to research applications, we demonstrated the effective use of the M kit for on-site test at the hospital and for testing clinical samples from chronic obstructive pulmonary disease patient. Thus, this developed M kit provides an easy and integrated experimental platform for cell migration related research and potential medical diagnostic applications.

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“…This feature makes the microdroplet technology extremely useful for biological applications including the high-throughput investigation of single cells and droplet digital polymerase chain reactions (ddPCR) (Agresti et al, 2010;Baker, 2012;Duncombe et al, 2015;Hindson et al, 2011;Rakszewska et al, 2014;Zinchenko et al, 2014). Droplet microfluidics is also useful for drug research (Sackmann et al, 2014;Vladisavljević et al, 2013), food industry (Neethirajan et al, 2011;Ushikubo et al, 2014;Zanatta et al, 2017), and material sciences for the synthesis and investigation of micro-and nanomaterials such as, e.g.…”

Section: Applications Of Droplet Microfluidicsmentioning

confidence: 99%

Droplet Microfluidics as a Tool for the Generation of Granular Matters and Functional Emulsions

Opalski

Kamiński

Garstecki

2019

KONA

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Emulsion-a liquid dispersed in another liquid-is in many respects very similar to granular matter. In the early 2000s a new technology-droplet microfluidics-began emerging from the wider field of microfluidics. Droplet microfluidics was quickly established as a discipline of science and engineering and has been used for the generation of highly uniform emulsions. The last few years have brought significant advances to the field, directed towards a wide range of applications in material sciences-from synthesis of nanoparticles in droplets to assembling complex droplets and using droplets as templates for ordered materials, with applications in food, cosmetic and diagnostic industries. Droplet microfluidic platforms are also successfully used as analytical tools in molecular biology and biochemistry, in e.g. high-throughput screening, digital assays, encapsulation of single cells, sequencing technologies, and in point-of-care diagnostic applications. This article provides an accessible overview of the physical phenomena observed in multiphase flow at the microscale and the techniques in droplet microfluidics systems. We also present the most interesting applications and potential further directions of research in this fascinating young field of science and engineering.

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Microfluidics: reframing biological enquiry

Cited by 278 publications

References 148 publications

Consumer-friendly food allergen detection: moving towards smartphone-based immunoassays

Consumer-friendly food allergen detection: moving towards smartphone-based immunoassays

Mkit: A cell migration assay based on microfluidic device and smartphone

Droplet Microfluidics as a Tool for the Generation of Granular Matters and Functional Emulsions

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