Microfluidic systems for rapid antibiotic susceptibility tests (ASTs) at the single-cell level

Zhang, Kaixiang; Qin, Shangshang; Wu, Shuangyan; Liang, Yan; Li, Jinghong

doi:10.1039/d0sc01353f

Cited by 59 publications

(50 citation statements)

References 81 publications

(75 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Implementing microfluidic approaches for advanced and accurate in vivo imaging of bacterial colony dynamics and digestion kinetics opens the path to enhance the information content, throughput, and versatility of assays targeting C. elegans microbiota–gut interaction. For instance, microfluidic platforms would be especially suitable for parallel on-chip assays based on worm feeding with different bacterial strains and/or the application of different antimicrobial compounds [ 62 ]. Likewise, a range of C. elegans mutants, presenting specific drug-dependent microbial–host interaction features, could be observed simultaneously.…”

Section: Discussionmentioning

confidence: 99%

An In Vivo Microfluidic Study of Bacterial Load Dynamics and Absorption in the C. elegans Intestine

et al. 2021

View full text Add to dashboard Cite

Caenorhabditiselegans (C. elegans) has gained importance as a model for studying host-microbiota interactions and bacterial infections related to human pathogens. Assessing the fate of ingested bacteria in the worm’s intestine is therefore of great interest, in particular with respect to normal bacterial digestion or intestinal colonization by pathogens. Here, we report an in vivo study of bacteria in the gut of C. elegans. We take advantage of a polydimethylsiloxane (PDMS) microfluidic device enabling passive immobilization of adult worms under physiological conditions. Non-pathogenic Escherichia coli (E. coli) bacteria expressing either pH-sensitive or pH-insensitive fluorescence reporters as well as fluorescently marked indigestible microbeads were used for the different assays. Dynamic fluorescence patterns of the bacterial load in the worm gut were conveniently monitored by time-lapse imaging. Cyclic motion of the bacterial load due to peristaltic activity of the gut was observed and biochemical digestion of E. coli was characterized by high-resolution fluorescence imaging of the worm’s intestine. We could discriminate between individual intact bacteria and diffuse signals related to disrupted bacteria that can be digested. From the decay of the diffuse fluorescent signal, we determined a digestion time constant of 14 ± 4 s. In order to evaluate the possibility to perform infection assays with our platform, immobilized C. elegans worms were fed pathogenic Mycobacterium marinum (M. marinum) bacteria. We analyzed bacterial fate and accumulation in the gut of N2 worms and mitochondrial stress response in a hsp-6::gfp mutant.

show abstract

Section: Discussionmentioning

confidence: 99%

An In Vivo Microfluidic Study of Bacterial Load Dynamics and Absorption in the C. elegans Intestine

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Microfluidic-based methods are a promising tool 2016) American Society for Microbiology). f MSP isolated microorganisms from deep-sea sediments samples ◂ for rapid ASTs because of their miniaturization and automation (Zhang et al 2020). Various microfluidics-based methods have been developed for ASTs, such as microchamber, microchannel, and droplet-based tools (Hassan and Zhang 2020).…”

Section: Antibiotic Susceptibility Testing In Dropletsmentioning

confidence: 99%

One cell at a time: droplet-based microbial cultivation, screening and sequencing

et al. 2021

Mar Life Sci Technol

View full text Add to dashboard Cite

Microbes thrive and, in turn, influence the earth's environment, but most are poorly understood because of our limited capacity to reveal their natural diversity and function. Developing novel tools and effective strategies are critical to ease this dilemma and will help to understand their roles in ecology and human health. Recently, droplet microfluidics is emerging as a promising technology for microbial studies with value in microbial cultivating, screening, and sequencing. This review aims to provide an overview of droplet microfluidics techniques for microbial research. First, some critical points or steps in the microfluidic system are introduced, such as droplet stabilization, manipulation, and detection. We then highlight the recent progress of droplet-based methods for microbiological applications, from high-throughput single-cell cultivation, screening to the targeted or whole-genome sequencing of single cells.

show abstract

“…However, traditional culture-based methods are usually time-consuming and tedious ( Forne et al, 2000 ; Guan et al, 2013 ). Currently, novel biosensors based diagnostic strategies have been developed with an improved sensitivity and accuracy ( Ren et al, 2019 ; Song et al, 2019 ; Yoo and Lee, 2016 ; Zhang et al, 2020 ). Those sensors include ( Hong et al, 2020 ; Wan et al, 2018 ; Wang and Alocilja, 2015 ; Wu et al, 2015 ) enzyme-based reporters [horseradish peroxidase (HRP) ( Wen et al, 2016 ), alkaline phosphatase (ALP) ( Han et al, 2020 ), and catalase ( de la Rica and Stevens, 2012 )], fluorescent molecule-based reporters [Fluorescein probes ( Cardoso et al, 2011 ) and Molecular Beacons ( Cao et al, 2014 )] and nanomaterial-based reporters [magneto-DNA nanoparticle system ( Chung et al, 2013 ), Gold nanoparticles ( Yang et al, 2020 )].…”

Section: Introductionmentioning

confidence: 99%

Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics

Song

Shen

Wei

et al. 2021

Biosensors and Bioelectronics

Self Cite

View full text Add to dashboard Cite

Microfluidic systems for rapid antibiotic susceptibility tests (ASTs) at the single-cell level

Abstract:
Recent developments of microfluidics-based antibiotic susceptibility tests (ASTs) at the single-cell or single-molecule level are summarized for guiding antibiotic treatment.

Cited by 59 publications

References 81 publications

An In Vivo Microfluidic Study of Bacterial Load Dynamics and Absorption in the C. elegans Intestine

An In Vivo Microfluidic Study of Bacterial Load Dynamics and Absorption in the C. elegans Intestine

One cell at a time: droplet-based microbial cultivation, screening and sequencing

Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics

Contact Info

Product

Resources

About

Microfluidic systems for rapid antibiotic susceptibility tests (ASTs) at the single-cell level

Abstract: Recent developments of microfluidics-based antibiotic susceptibility tests (ASTs) at the single-cell or single-molecule level are summarized for guiding antibiotic treatment.

Cited by 59 publications

References 81 publications

An In Vivo Microfluidic Study of Bacterial Load Dynamics and Absorption in the C. elegans Intestine

An In Vivo Microfluidic Study of Bacterial Load Dynamics and Absorption in the C. elegans Intestine

One cell at a time: droplet-based microbial cultivation, screening and sequencing

Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics

Contact Info

Product

Resources

About

Abstract:
Recent developments of microfluidics-based antibiotic susceptibility tests (ASTs) at the single-cell or single-molecule level are summarized for guiding antibiotic treatment.