The green treatment of dye wastewater has always been a research hotspot in the environmental field. The photocatalytic technology is considered to be a simple and effective strategy to remove...
Nanopore
technology is promising for the next-generation of nucleic
acid–based diagnosis. However, sequence reservation could still
be hardly achieved in low-concentration. Herein, we propose a trypsin-activated
catalysis reaction for amplified detection, which substantially improves
the sensitivity of nanopore technique. The proposed trypsin-amplified
nanopore amplified sandwich assay (tNASA) could contribute to a sensitivity
approximately 100 000 times higher based on nucleic acid probe
design. Remarkably, tNASA is capable of attomolar nucleic acid and
single cell detection by using a miniaturized current amplifier without
alignment algorithm. Also it allows 10 pathogenic species in serum
to be accurately and robustly profiled, thus be utilized for the diagnosis
of infectious diseases. tNASA may evolve the construction of nanopore
techniques for nucleic acid detection and would facilitate its translation
for pocket diagnosis and precision medicine.
Field-deployable
detectors of disease biomarkers provide a simple
and fast analysis of clinical specimens. However, most of the existing
field-deployable diagnostics have poor sensitivity and are not suitable
for the detection of biomarkers with low abundance. Herein, we report
a highly sensitive and rapid colorimetric readout paper-based assay
for pathogen detection by integrating the unique collateral activity
of a Cas12a-activated universal field-deployable detector (CUFD).
The collateral effect of Cas12a results in a nonspecific destruction
of a fluorophore biotin-labeled ssDNA reporter for the CUFD. This
technique can quantify seven different kinds of pathogens in blood
samples without any purification procedure, with sensitivity as low
as 10 aM for the Shigella dysenteriae DNA. This CUFD technique has significant potential for the detection
of pathogenic DNA as well as other types of DNA or RNA targets at
the point-of-care application.
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