Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics

Song, Fengge; Shen, Yuanyuan; Wei, Yangdao; Yang, Chunrong; Ge, Xiaolin; Wang, Aimin; Li, Chaoyang; Wan, Yi; Li, Jinghong

doi:10.1016/j.bios.2020.112953

Cited by 10 publications

(12 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[63] Especially, aptamer-dependent sensors combining with QDs could reach extreme low detection limit to single cell level. [166,179] In addition, DNA could also be an indicator of the existence of bacteria. [134,157] As depicted in Figure 5C, an FRET mechanism for detecting of antibiotic resistance genes was employed.…”

Section: Electrostatic Interactions and Ros Generationmentioning

confidence: 99%

“…FRET is defined as the process of the excited state segment transports the energy to the grand state [113,154,[164][165][166] along with fluorescence quenching. The excited state is the donor, which can act as a probe for sensing, and the grand state is the accepter.…”

Section: Formation and Operating Mechanisms Of Sensing Elementsmentioning

confidence: 99%

“…Besides, a bacterial identification device was also fabricated for monitoring E. coli and Pseudomonas aeruginosa through a simple fluorescent marking method, [177] which could practice automatic real-time photoelectric sensing with a detection limit of 1 cfu mL −1 . Direct detection may not be always realized, so that substances referring to bacteria like enzyme, [64] nucleic acid, [134] exotoxin, [166] or endotoxin [178] can be treated as the detecting target. For example, micrococcal nuclease, is an extracellular endonuclease of S. aureus, was designed to be detected through an immune fluorescent strip with 0.5 ng mL −1 of LOD.…”

Section: Electrostatic Interactions and Ros Generationmentioning

confidence: 99%

See 2 more Smart Citations

Quantum Dots for Pathogenic Bacterial Monitoring and Combating

Liu

Zhang

2022

Advanced Optical Materials

View full text Add to dashboard Cite

including neonatal meningitis, [6] diarrhoea, [7] infected cutaneous wound [8] or bloodstream diseases, [9][10][11] etc. Antibiotic treatment strategies against these bacteria have been widely applied while antimicrobial resistance has been arousing gradually. [12][13][14][15][16][17] What is worse, the biofilmrelated infection has been a tough issue for bacterial infectious treatment since it is usually chronic and much harder to eradicate. [18][19][20] Therefore, effective antibacterial treatments for drug-resistant pathogenic bacteria are vital problems for clinical therapy. [21] Some anti-microbial trials were carried out like phages, bacteriocins, and antimicrobial peptides [22] along with various therapeutic methods. [23][24][25] Among them, nanomaterials are considered as compatible solutions [23,26] due to their facile synthesis methods and rich-sourced precursors, broad-spectrum sterilization, and low drug resistance. [27][28][29][30][31] Nanomaterials are generally easy to be modified or doped to enhance the affinity toward bacteria for the sake of combating or drug delivery. [32][33][34][35] The antibacterial mechanism [36,37] could be result from the sharp edges-based physical cutting, [38,39] photothermal effects, [40][41][42] and photo dynamic or chemical dynamic properties, that could produce reactive oxygen species (ROS).Pathogenic bacteria monitoring mainly conducted by culturing them in agar-based media, and subsequently testing them with biochemical probes. [43][44][45][46] Although this conventional method enables clinical diagnoses with relatively reliable outcomes, the long culturing time renders the "golden therapeutical window" for patients. So more rapid and accurate detecting technologies have been invented. One of the most evolutionary methods is the polymerase chain reaction, [47][48][49] which can identify the bacterial classification and provide gene expression level in a few hours with better accuracy. [47,50] Besides, the coupling of chromatography and mass spectra like matrix-assisted laser desorption ionization time-of-flight mass spectrometry [51][52][53][54][55] can play a more exact role in diagnosis. [56] Though these advanced instrumental-relied methods can lead to fast diagnostic, the expensive apparatus, environmentally unfriendly reagents, [57] professional sites and technical personnel requirements limited their utilization in real-time monitoring and point-of-care sensing. By contrast, easy and fast monitoring methods for the pathogen, such as electrochemical and fluorescence sensors, were brought out as powerful supplements. [58][59][60][61][62] Therein, Antibiotic resistance and pathogenic bacterial monitoring are two main challenges in clinical diagnostics and treatment of bacteria. To conquer those issues, many new materials and novel technologies are explored. Among them, quantum dots (QDs) are regarded as not only powerful bacteriostatic agents, but also bacterial monitoring probes due to their excellent photoluminescence properties, tunable multiwavelength lumine...

show abstract

Section: Electrostatic Interactions and Ros Generationmentioning

confidence: 99%

Section: Formation and Operating Mechanisms Of Sensing Elementsmentioning

confidence: 99%

Section: Electrostatic Interactions and Ros Generationmentioning

confidence: 99%

See 1 more Smart Citation

Quantum Dots for Pathogenic Bacterial Monitoring and Combating

Liu

Zhang

2022

Advanced Optical Materials

View full text Add to dashboard Cite

show abstract

“… Testing approaches Testing type Sample source Gene/region detection LOD Detection time Target analyte References Colorimetric biosensor based on gold nanoparticle (AuNP) POC Nasal swabs, RdRp and N genes Threshold cycle (Ct) = 36.5 3 min Spike protein, membrane protein, envelope proteins 142 Colorimetric biosensor based on botulinum neurotoxin receptor POC Blood, urine, Stool and Nasopharyngeal swabs N genes 1a.m. 1–2 h Recombinant plasmid 143 RT-PCR colorimetric assay Laboratory based Upper respiratory specimens ORF1ab and N genes 10 5 dilution factors Total ∼2–3 h But observable color change found within 10 min Nucleic acids template 144 Colorimetric detection using CRISPER/dCas9 Laboratory based Nasopharyngeal aspirates and sputum specimens N1, N2, and N3 genes 30 pM ∼2 h Nucleic acids 145 Colorimetric detection using DNAzyme sensor Laboratory and POC-based Swabs from nasopharyngeal and oropharyngeal N genes 10 1 to 10 7 copies Total duration ∼1 h and visual observation ∼5 min RNA 146 Paper-based colorimetric biosensor using electrochemical assay Laboratory and POC-based Not mentioned Immunoglobulins IgG and IgM ∼1 ng/mL 30 min Spike protein 136 Colorimetric biosensor using LAMP assay POC Nasal swabs N gene ∼62.5 viral copies/LAMP reaction 50–60 min Plasmids 147 Colorimetric detection using RT-LAMP …”

Section: Nanobiosensing Techniques For Sars-cov-2 Detectionmentioning

confidence: 99%

Public health management during COVID-19 and applications of point-of-care based biomolecular detection approaches

Dastidar

Roy

2021

Environmental and Health Management of Novel Coronavirus Disease (COVID-19 )

View full text Add to dashboard Cite

“…Therefore, the early diagnosis of infection and cutting off the transmission route are the keys to effectively controlling the epidemic. At the same time, the development of rapid, sensitive, convenient, and accurate virus detection technology is imminent [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances of Representative Optical Biosensors for Rapid and Sensitive Diagnostics of SARS-CoV-2

Lin

et al. 2022

Biosensors

View full text Add to dashboard Cite

The outbreak of Corona Virus Disease 2019 (COVID-19) has again emphasized the significance of developing rapid and highly sensitive testing tools for quickly identifying infected patients. Although the current reverse transcription polymerase chain reaction (RT-PCR) diagnostic techniques can satisfy the required sensitivity and specificity, the inherent disadvantages with time-consuming, sophisticated equipment and professional operators limit its application scopes. Compared with traditional detection techniques, optical biosensors based on nanomaterials/nanostructures have received much interest in the detection of SARS-CoV-2 due to the high sensitivity, high accuracy, and fast response. In this review, the research progress on optical biosensors in SARS-CoV-2 diagnosis, including fluorescence biosensors, colorimetric biosensors, Surface Enhancement Raman Scattering (SERS) biosensors, and Surface Plasmon Resonance (SPR) biosensors, was comprehensively summarized. Further, promising strategies to improve optical biosensors are also explained. Optical biosensors can not only realize the rapid detection of SARS-CoV-2 but also be applied to judge the infectiousness of the virus and guide the choice of SARS-CoV-2 vaccines, showing enormous potential to become point-of-care detection tools for the timely control of the pandemic.

show abstract

Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics

Cited by 10 publications

References 43 publications

Quantum Dots for Pathogenic Bacterial Monitoring and Combating

Quantum Dots for Pathogenic Bacterial Monitoring and Combating

Public health management during COVID-19 and applications of point-of-care based biomolecular detection approaches

Recent Advances of Representative Optical Biosensors for Rapid and Sensitive Diagnostics of SARS-CoV-2

Contact Info

Product

Resources

About