Microfluidic Systems for Neural Cell Studies

Babaliari, Eleftheria; Ranella, Anthi; Stratakis, Emmanuel

doi:10.3390/bioengineering10080902

Cited by 4 publications

(1 citation statement)

References 151 publications

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“…Novel extracellular matrix analogs, microfluidic systems, and 3D bioprinting are improving organoid viability, function, and structure, enabling the creation of more sophisticated models for tumor studies and personalized medicine ( Xu et al, 2019b ; Yu et al, 2022 ). Additionally microfluidic systems enable dynamic culturing with continuous nutrient supply and waste removal and further improving the physiological relevance of these models ( Babaliari et al, 2023 ). Figure 3 illustrates the current and future applications of PDOs.…”

Section: Organoids In Cancer Research: Current Applicationsmentioning

confidence: 99%

The future of cancer therapy: exploring the potential of patient-derived organoids in drug development

Avci,

Bagca,

Shademan

et al. 2024

Front. Cell Dev. Biol.

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Cancer therapy is on the brink of a significant transformation with the inclusion of patient-derived organoids (PDOs) in drug development. These three-dimensional cell cultures, directly derived from a patient’s tumor, accurately replicate the complex structure and genetic makeup of the original cancer. This makes them a promising tool for advancing oncology. In this review, we explore the practical applications of PDOs in clinical drug screening and pharmacognostic assessment, as well as their role in refining therapeutic strategies. We provide insights into the latest advancements in PDO technology and its implications for predicting treatment responses and facilitating novel drug discoveries. Additionally, we address the operational challenges associated with incorporating PDOs into the drug development process, such as scaling up organoid cultures, ensuring consistent results, and addressing the ethical use of patient-derived materials. Aimed at researchers, clinicians, and key stakeholders in oncology, this article aims to succinctly present both the extraordinary potential and the obstacles to integrating PDOs, thereby shedding light on their prospective impact on the future of cancer treatment.

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Section: Organoids In Cancer Research: Current Applicationsmentioning

confidence: 99%

The future of cancer therapy: exploring the potential of patient-derived organoids in drug development

Avci,

Bagca,

Shademan

et al. 2024

Front. Cell Dev. Biol.

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In Vitro Models for Peripheral Nerve Regeneration

Tusnim,

Kutuzov,

Grasman

2024

Adv Healthcare Materials

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Peripheral nerve injury (PNI) resulting in lesions is highly prevalent clinically, but current therapeutic approaches fail to provide satisfactory outcomes in many patients. While peripheral nerves have intrinsic regenerative capacity, the regenerative capabilities of peripheral nerves are often insufficient to restore full functionality. This highlights an unmet need for developing more effective strategies to repair damaged peripheral nerves and improve regenerative success. Consequently, researchers are actively exploring a variety of therapeutic strategies, encompassing the local delivery of trophic factors or bioactive molecules, the design of advanced biomaterials that interact with regenerating axons, and augmentation with nerve guidance conduits or complex prostheses. However, clinical translation of these technologies remains limited, emphasizing the need for continued research on peripheral nerve regeneration modalities that can enhance functional restoration. Experimental models that accurately recapitulate key aspects of peripheral nerve injury and repair biology can accelerate therapeutic development by enabling systematic testing of new techniques. Advancing regenerative therapies for PNI requires bridging the gap between basic science discoveries and clinical application. This review discusses different in vitro models of peripheral nerve injury and repair, including their advantages, limitations, and potential applications.

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Impact of perfusion on neuronal development in human derived neuronal networks

Di Lisa,

Andolfi,

Masi

et al. 2024

APL Bioengineering

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Advanced in vitro models of the brain have evolved in recent years from traditional two-dimensional (2D) ones, based on rodent derived cells, to three-dimensional (3D) ones, based on human neurons derived from induced pluripotent stem cells. To address the dynamic changes of the tissue microenvironment, bioreactors are used to control the in vitro microenvironment for viability, repeatability, and standardization. However, in neuronal tissue engineering, bioreactors have primarily been used for cell expansion purposes, while microfluidic systems have mainly been employed for culturing organoids. In this study, we explored the use of a commercial perfusion bioreactor to control the culture microenvironment of neuronal cells in both 2D and 3D cultures. Namely, neurons differentiated from human induced pluripotent stem cells (iNeurons) were cultured in 2D under different constant flow rates for 72 h. The impact of different flow rates on early-stage neuronal development and synaptogenesis was assessed by morphometric characterization and synaptic analysis. Based on these results, two involving variable flow rates were developed and applied again in 2D culture. The most effective protocol, in terms of positive impact on neuronal development, was then used for a preliminary study on the application of dynamic culturing conditions to neuronal cells in 3D. To this purpose, both iNeurons, co-cultured with astrocytes, and the human neuroblastoma cells SH-SY5Y were embedded into a hydrogel and maintained under perfusion for up to 28 days. A qualitative evaluation by immunocytochemistry and confocal microscopy was carried out to assess cell morphology and the formation of a 3D neuronal network.

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Microfluidic Systems for Neural Cell Studies

Cited by 4 publications

References 151 publications

The future of cancer therapy: exploring the potential of patient-derived organoids in drug development

The future of cancer therapy: exploring the potential of patient-derived organoids in drug development

In Vitro Models for Peripheral Nerve Regeneration

Impact of perfusion on neuronal development in human derived neuronal networks

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