Microfluidic chips for chirality exploration

Nagl, Stefan; Schulze, Philipp; Ohla, Stefan; Beyreiss, Reinhild; Gitlin, Leonid; Belder, Detlev

doi:10.1021/ac200150w

Cited by 30 publications

(19 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After we could prove the performance and the stability of the PEG-coated chips, we investigated their application to improve chiral separations in MCE [47][48][49]. As test compounds we chose FITC-labelled amino acid enantiomers using b-cyclodextrin (b-CD) as chiral selector.…”

Section: Resultsmentioning

confidence: 99%

Poly(ethylene glycol)‐coated microfluidic devices for chip electrophoresis

Schulze

Belder

2012

Electrophoresis

Self Cite

View full text Add to dashboard Cite

Herein, we report on a strategy for durable modification of the channel surface in microfluidic glass chips with the neutral hydrophilic-coating material poly(ethylene glycol) PEG-1M-100. Applied in microchip electrophoresis such PEG-coated devices exhibit a suppressed electroosmotic flow and reduced analyte adsorption. The PEG-coated chips were successfully applied in chip electrophoresis of FITC-labelled amines and amino acids and native proteins as well as in chiral separations. The performance of the coated chips was found to be superior compared with uncoated microchips. The coated chips exhibited high stability and the relative standard deviation of migration times in PEG-coated devices was less than 2%.

show abstract

Section: Resultsmentioning

confidence: 99%

Poly(ethylene glycol)‐coated microfluidic devices for chip electrophoresis

Schulze

Belder

2012

Electrophoresis

Self Cite

View full text Add to dashboard Cite

show abstract

“…High space-time yields, high selectivity, and complete conversion are key goals in process intensification and resource efficiency. Microreactors are also of interest for rapid process development if PAT matches the reaction timescale [79,80]. Real-time product analysis is key for quality by design (QbD) of the manufacturing process.…”

Section: Process Integrationmentioning

confidence: 99%

Microscale technology and biocatalytic processes: opportunities and challenges for synthesis

Wohlgemuth¹,

Plazl

Žnidaršič–Plazl

et al. 2015

Trends in Biotechnology

180

151

View full text Add to dashboard Cite

“…They offer good peak capacity and sensitivity, low sample handling, minute sample requirements and are portable, giving the opportunity to perform field analysis. Mostly, microdevices are used for electrophoretic analysis, since the conditions to realize this kind of separations (liquid media, application of a voltage) are easy to realize for such small devices . However, the introduction of monolithic materials has given a new impetus to the preparation of microdevices for chromatographic purposes.…”

Section: Nano‐lcmentioning

confidence: 99%

Enantiomeric separations by means of nano‐LC

Rocco

Maruška

Fanali³

2013

J of Separation Science

View full text Add to dashboard Cite

Enantiomers represent a class of compounds extensively investigated since they can show totally different behaviors when they interact with a chiral environment. Because of their identical chemical structure (they differ only in the spatial arrangement of the atoms in the molecule), the separation of optical isomers is a challenging task of analytical chemistry. So far employed methods for the separation of enantiomers are mainly based on chromatography. CE as well was considered as an analytical technique suitable for chiral separations, characterized by high efficiency and low consumption of reagent. Recently, miniaturization was introduced in LC to answer the needs to perform analyses in the minimum time, to use the smallest amount of samples and to reduce environmental pollution. Nano-LC represents nowadays a valid alternative to the abovementioned conventional analytical techniques, and can be advantageously exploited for enantiomeric separation especially because it needs minute amounts of the chiral material necessary to carry out enantiomeric separations. This review describes the development and applications of nano-LC in the field of chiral separations. The data reported in literature show its relevance for the study enantiomers-chiral selectors interaction, as well as for application in pharmaceutical and clinical research.

show abstract

Microfluidic chips for chirality exploration

Abstract: Microfluidic chips applied to the investigation of chirality allow reaction, separation and analysis of minuscule amounts of enantiomeric molecules. Chiral chip technology is employed in fields as diverse as pharmaceutical high throughput screening and deep space exploration missions.

Cited by 30 publications

References 56 publications

Poly(ethylene glycol)‐coated microfluidic devices for chip electrophoresis

Poly(ethylene glycol)‐coated microfluidic devices for chip electrophoresis

Microscale technology and biocatalytic processes: opportunities and challenges for synthesis

Enantiomeric separations by means of nano‐LC

Contact Info

Product

Resources

About