Microscale technology and biocatalytic processes: opportunities and challenges for synthesis

Wohlgemuth, Roland; Plazl, Igor; Žnidaršič–Plazl, Polona; Gernaey, Krist V.; Woodley, John M.

doi:10.1016/j.tibtech.2015.02.010

Cited by 171 publications

(151 citation statements)

References 88 publications

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“…A strategic advantage is also the potential to perform multiple reactions and explore a broad range of reaction conditions in a fast and affordable way, as minor amounts of reagents and solutions are utilized while waste production is minimized. Other reaction boundaries like substrate toxicity and catalyst deactivation may also be overcome as a result of the continuous operation mode of such microscale systems because products are removed and substrates are injected constantly [2,3].…”

Section: Introductionmentioning

confidence: 99%

Magnetic Microreactors with Immobilized Enzymes—From Assemblage to Contemporary Applications

2018

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Microfluidics, as the technology for continuous flow processing in microscale, is being increasingly elaborated on in enzyme biotechnology and biocatalysis. Enzymatic microreactors are a precious tool for the investigation of catalytic properties and optimization of reaction parameters in a thriving and high-yielding way. The utilization of magnetic forces in the overall microfluidic system has reinforced enzymatic processes, paving the way for novel applications in a variety of research fields. In this review, we hold a discussion on how different magnetic particles combined with the appropriate biocatalyst under the proper system configuration may constitute a powerful microsystem and provide a highly explorable scope.

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Section: Introductionmentioning

confidence: 99%

Magnetic Microreactors with Immobilized Enzymes—From Assemblage to Contemporary Applications

2018

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“…In the specific case of multi‐enzyme reactions, microreactors offer straightforward and efficient compartmentalization of the reactions (Gruber, Marques, O'Sullivan et al, 2017). Furthermore, reaction and separation steps can be combined (O'Sullivan et al, 2012) and throughput can be increased via parallelization of the microreactors (Bolivar, Wiesbauer, & Nidetzky, 2011; Krühne et al, 2014; Wohlgemuth, Plazl, Žnidaršič‐Plazl, Gernaey, & Woodley, 2015). These advantages may lead to faster biocatalytic process development or the creation of novel synthetic routes, while at the same time reducing cost and environmental impact.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic synthesis of chiral amino‐alcohols by coupling transketolase and transaminase‐catalyzed reactions in a cascading continuous‐flow microreactor system

Gruber

Carvalho

Marques

et al. 2017

Biotech & Bioengineering

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Rapid biocatalytic process development and intensification continues to be challenging with currently available methods. Chiral amino‐alcohols are of particular interest as they represent key industrial synthons for the production of complex molecules and optically pure pharmaceuticals. (2S,3R)‐2‐amino‐1,3,4‐butanetriol (ABT), a building block for the synthesis of protease inhibitors and detoxifying agents, can be synthesized from simple, non‐chiral starting materials, by coupling a transketolase‐ and a transaminase‐catalyzed reaction. However, until today, full conversion has not been shown and, typically, long reaction times are reported, making process modifications and improvement challenging. In this contribution, we present a novel microreactor‐based approach based on free enzymes, and we report for the first time full conversion of ABT in a coupled enzyme cascade for both batch and continuous‐flow systems. Using the compartmentalization of the reactions afforded by the microreactor cascade, we overcame inhibitory effects, increased the activity per unit volume, and optimized individual reaction conditions. The transketolase‐catalyzed reaction was completed in under 10 min with a volumetric activity of 3.25 U ml−1. Following optimization of the transaminase‐catalyzed reaction, a volumetric activity of 10.8 U ml−1 was attained which led to full conversion of the coupled reaction in 2 hr. The presented approach illustrates how continuous‐flow microreactors can be applied for the design and optimization of biocatalytic processes.

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“…The significance of continuous-flow biocatalytic microreactors has been evaluated for both enzyme-and whole cell-based catalytic reactions [5,6]. The key advantage of maximizing the mass transfer rate and consequently the reaction performance by the application of continuous-flow microreactors has been well demonstrated for multiphasic enzymatic reactions [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…The key advantage of maximizing the mass transfer rate and consequently the reaction performance by the application of continuous-flow microreactors has been well demonstrated for multiphasic enzymatic reactions [6,7]. In such multiphasic flow conditions, the chosen flow pattern defines mixing and mass transfer.…”

Section: Introductionmentioning

confidence: 99%

CatalyticPseudomonas taiwanensisVLB120ΔC biofilms thrive in a continuous pure styrene generated by multiphasic segmented flow in a capillary microreactor

Halan¹,

Karande²,

Buehler³

et al. 2016

Journal of Flow Chemistry

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This study investigated the survival and catalytic potential of a single species Pseudomonas taiwanensis VLB120∆C biofilm for the conversion of styrene to (S)-styrene oxide in a multiphasic capillary microreactor containing the highly toxic substrate styrene as a pure phase. The catalytic biofilm was cultivated under high fluidic stress in a continuous three-phase segmented flow system comprising aqueous medium, air, and styrene. This concept required an adaptation period of 7 days, during which P. taiwanensis VLB120∆C developed a biofilm exhibiting a remarkable cellular integrity with nearly 70% intact cells. In a three-phase segmented flow biofilm microreactor, an average specific styrene epoxidation rate of 10 g/L tube /day was achieved continuously for a period of 20 days without any clogging problems. Overall, this note highlights the robustness of biofilms as a promising biocatalyst format for the conversion/synthesis of toxic organic chemicals and the applicability of multiphasic capillary microreactors for biofilm based catalysis.

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Microscale technology and biocatalytic processes: opportunities and challenges for synthesis

Cited by 171 publications

References 88 publications

Magnetic Microreactors with Immobilized Enzymes—From Assemblage to Contemporary Applications

Magnetic Microreactors with Immobilized Enzymes—From Assemblage to Contemporary Applications

Enzymatic synthesis of chiral amino‐alcohols by coupling transketolase and transaminase‐catalyzed reactions in a cascading continuous‐flow microreactor system

CatalyticPseudomonas taiwanensisVLB120ΔC biofilms thrive in a continuous pure styrene generated by multiphasic segmented flow in a capillary microreactor

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