Microcontact printing of proteins for neuronal cell guidance

Abstract

The growth of neurons into networks of controlled geometry is of great interest in the field of cellbased biosensors, neuroelectronic circuits, neurological implants, pharmaceutical testing as well as fundamental biological questions about neuronal interactions. The precise control of the network architecture can be achieved by defined engineering of the surface material properties: this process is called neuronal cell patterning. Different techniques can be used to produce such surface patterns. We have chosen microcontact printing (mCP), because it is a comparatively simple and universal method for patterning biomolecules.

Cell placement and guidance on substrates for neurochip interfaces

“…This can be achieved using an established stamping technique where a patterned PDMS stamp is used to transfer chemicals on to the substrate (Bani-Yaghoub et al, 2005;Charrier et al, 2006;Kumar et al, 1994;Offenhausser et al, 2007;Vogt et al, 2005;Wyart et al, 2002). In this study, we used poly-D-lysine (PDL), a commonly used cell attachment factor (Branch et al, 1998;Chang et al, 2003;Li and Folch, 2005).…”

Elastin‐Like Macromolecules

“…Using lithography and patterning techniques, peptides and proteins can be deposited with absolute spatial control on specific regions of a surface [174,175]. The ability to obtain nano-and microstructured surfaces of patterned biomacromolecules is of great importance for several applications, including biological assays, miniaturized biosensors, and biomedical diagnostics.…”

SERS Immunoassay Using Microcontact Printing for Application of Sensitive Biosensors

“…The stamp was then removed from the silicon substrate surface. [33][34][35] For patterned gold nanoparticles, the substrate was prepared by using the self-assembled monolayer (SAM) technique. An APTMS-derivatized surface on the OTS patterned substrate was also prepared by using the SAM technique.…”