SERS Immunoassay Using Microcontact Printing for Application of Sensitive Biosensors

Hong, Wonjin; Jung, Young-Mee

doi:10.5012/bkcs.2011.32.12.4281

Cited by 4 publications

(7 citation statements)

References 40 publications

(28 reference statements)

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“…(3) Their Raman spectra should have few characteristic peaks, which can minimize the band overlap with other reporters in multiplex detection. Up to now, various molecules have served as Raman reporters, ranging from small organic molecules [such as 4-mercaptobenzoic acid (4MBA) 73 and rhodamine 6G (R6G) 74 ] to inorganic materials (such as graphene oxide 75 and carbon nanotube 76 ). The diversity of Raman spectra offers wide choices for fabricating SERS nanoprobes.…”

Section: Chemical Reviewsmentioning

confidence: 99%

SERS-Activated Platforms for Immunoassay: Probes, Encoding Methods, and Applications

et al. 2017

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Owing to their excellent multiplexing ability, high sensitivity, and large dynamic range, immunoassays using surface-enhanced Raman scattering (SERS) as the readout signal have found prosperous applications in fields such as disease diagnosis, environmental surveillance, and food safety supervision. Various ever-increasing demands have promoted SERS-based immunoassays from the classical sandwich-type ones to those integrated with fascinating automatic platforms (e.g., test strips and microfluidic chips). As recent years have witnessed impressive progress in SERS immunoassays, we try to comprehensively cover SERS-based immunoassays from their basic working principles to specific applications. Focusing on several basic elements in SERS immunoassays, typical structures of SERS nanoprobes, productive optical spectral encoding strategies, and popular immunoassay platforms are highlighted, followed by their representative biological applications in the last 5 years. Moreover, despite the vast advances achieved to date, SERS immunoassays still suffer from some annoying shortcomings. Thus, proposals on how to improve the SERS immunoassay performance are also discussed, as well as future challenges and perspectives, aiming to give brief and valid guidelines for choosing suitable platforms according to particular applications.

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Section: Chemical Reviewsmentioning

confidence: 99%

SERS-Activated Platforms for Immunoassay: Probes, Encoding Methods, and Applications

et al. 2017

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“…To this end, one of the first reports regarding cancer markers immunochemical detection with SERS was about the determination of PSA through a non-competitive immunoassay onto a substrate patterned by microcontact printing to define areas modified with (3-aminopropyl)trimethoxysilane onto which Au nanoparticles could be attached [ 60 ]. The immobilized Au nanoparticles were then modified with an anti-PSA antibody, whereas Au nanoparticles modified with the Raman reporter molecule rhodamine 6G and an anti-PSA antibody were used as labels.…”

Section: Cancer Biomarkers Immunochemical Detection Via Sersmentioning

confidence: 99%

SERS Immunosensors for Cancer Markers Detection

et al. 2023

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Early diagnosis and monitoring are essential for the effective treatment and survival of patients with different types of malignancy. To this end, the accurate and sensitive determination of substances in human biological fluids related to cancer diagnosis and/or prognosis, i.e., cancer biomarkers, is of ultimate importance. Advancements in the field of immunodetection and nanomaterials have enabled the application of new transduction approaches for the sensitive detection of single or multiple cancer biomarkers in biological fluids. Immunosensors based on surface-enhanced Raman spectroscopy (SERS) are examples where the special properties of nanostructured materials and immunoreagents are combined to develop analytical tools that hold promise for point-of-care applications. In this frame, the subject of this review article is to present the advancements made so far regarding the immunochemical determination of cancer biomarkers by SERS. Thus, after a short introduction about the principles of both immunoassays and SERS, an extended presentation of up-to-date works regarding both single and multi-analyte determination of cancer biomarkers is presented. Finally, future perspectives on the field of SERS immunosensors for cancer markers detection are briefly discussed.

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“…A large group of proposed substrates includes glass, quartz, or silicon slides with a layer of nanoparticles, usually gold or silver, of various shapes (e.g., spherical, nanorods) [34][35][36][37][38][39][40][41]. To improve the intensity and repeatability of the SERS signal, substrates with a layer of silver or gold nanoparticles on microarray chips were also developed [42][43][44][45]; the most interesting case in this group was the use of ordered gold nanohoneycomb arrays [46]. The authors of various studies in the course of continuous development of and the search for new SERS substrates competed using increasingly original ideas.…”

Section: Solid-and Liquid-phase Sers-active Substratementioning

confidence: 99%

“…As a detection element in the sandwich sensors developed so far, the most frequently used were gold [9,24,28,31,34,35,37,38,43,45,47,56,58,61,63,70,77,78,80,83,84,87,91,94,95] and silver [9,27,40,55,60,65,79,82,88,92] nanoparticles (AuNPs and AgNPs) with a shape similar to a sphere and of various sizes. These nanoparticles were each functionalized with a selected Raman reporter and a detection antibody to enable identification and determination of cancer biomarkers.…”

Section: Nanospherical Sers-active Particlesmentioning

confidence: 99%

Recent Advances in Sandwich SERS Immunosensors for Cancer Detection

Pollap

Świt

2022

IJMS

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Cancer has been one of the most prevalent diseases around the world for many years. Its biomarkers are biological molecules found in the blood or other body fluids of people with cancer diseases. These biomarkers play a crucial role not only in the diagnosis of cancer diseases, but also in risk assessment, selection of treatment methods, and tracking its progress. Therefore, highly sensitive and selective detection and determination of cancer biomarkers are essential from the perspective of oncological diagnostics and planning the treatment process. Immunosensors are special types of biosensors that are based on the recognition of an analyte (antigen) by an antibody. Sandwich immunosensors apply two antibodies: a capture antibody and a detection antibody, with the antigen ‘sandwiched’ between them. Immunosensors’ advantages include not only high sensitivity and selectivity, but also flexible application and reusability. Surface-enhanced Raman spectroscopy, known also as the sensitive and selective method, uses the enhancement of light scattering by analyte molecules adsorbed on a nanostructured surface. The combination of immunosensors with the SERS technique further improves their analytical parameters. In this article, we followed the recent achievements in the field of sandwich SERS immunosensors for cancer biomarker detection and/or determination.

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SERS Immunoassay Using Microcontact Printing for Application of Sensitive Biosensors

Cited by 4 publications

References 40 publications

SERS-Activated Platforms for Immunoassay: Probes, Encoding Methods, and Applications

SERS-Activated Platforms for Immunoassay: Probes, Encoding Methods, and Applications

SERS Immunosensors for Cancer Markers Detection

Recent Advances in Sandwich SERS Immunosensors for Cancer Detection

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