Microcantilever-based label-free thermal characterization of biomolecular affinity binding

Wang, Bin; Huang, Fengliang; Nguyen, Thai; Lin, Qiao

doi:10.1109/memsys.2010.5442341

Cited by 6 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microcantilevers functionalized with metal-binding protein “AgNt84-6”, are demonstrated for the detection of HMIs like Hg2+ and Zn2+ by Cherian et al (2003); SAMs (self-assembled monolayer’s) modified microcantilevers used for detection of Ca2+ ions by Ji and Thundat (2002); Arrays of microcantilever sensors encapsulated in fluidic wells by Venstra et al (2012) and fluidic channel by Xiang and Lee (2009); A Chitosan (CS)-graphene oxide (GO) Surface Plasmon Resonance (SPR) sensor by Kamaruddin et al (2014); a microcantilever/diaphragm-based system Wang et al (2010), Feng et al (2013); Lang et al (2013) and Hwang et al (2017), but all of these methods use optical readout which is a cumbersome method and required costly lab equipment. The optical readout has some disadvantages when used with microcantilever-based biosensor in the microfluidic environment when the refractive index of liquid changes and when used in opaque liquid because it absorbs the laser light by Thaysen (2001).…”

Section: Introductionmentioning

confidence: 99%

Design of microfluidic experimental setup for the detection of heavy metal ions using piezoresistive BioMEMS sensor

Rotake

Darji

Kale

2019

View full text Add to dashboard Cite

Purpose This paper aims to propose a new microfluidic portable experimental platform for quick detection of heavy metal ions (HMIs) in picomolar range. The experimental setup uses a microfabricated piezoresistive sensor (MPS) array of eight cantilevers with ion-selective self-assembled monolayer's (SAM). Design/methodology/approach Most of the components used in this experimental setup are battery operated and, hence, portable to perform the on-field experiments. HMIs (antigen) and thiol-based SAM (antibody) interaction start bending the microcantilever. This results in a change of resistance, which is directly proportional to the surface stress produced due to the mass of targeted HMIs. The authors have used Cysteamine and 4-Mercaptobenzoic acid as a thiol for creating SAM to test the sensitivity and identify the suitable thiol. Some of the cantilevers are blocked using acetyl chloride to use as a reference for error detection. Findings The portable experimental platform achieves very small detection time of 10-25 min with a lower limit of detection (LOD) 0.762 ng (6.05 pM) for SAM of Cysteamine and 4-Mercaptobenzoic acid to detect Mn2+ ions. This technique has excellent potential and capability to selectively detect Hg2+ ions as low as 2.43 pM/mL using SAM of Homocysteine (Hcys)-Pyridinedicarboxylic acid (PDCA). Research limitations/implications As microcantilever is very thin and fragile, it is challenging to apply a surface coating to have selective detection using Nanadispenser. Some of the cantilevers get broken during this process. Originality/value The excessive use and commercialization of NPs are quickly expanding their toxic impact on health and the environment. Also, LOD is limited to nanomolar range. The proposed method used the combination of thin-film, NPs, and MEMS-based technology to overcome the limitation of NPs-based technique and have picomolar range of HMIs detection.

show abstract

Section: Introductionmentioning

confidence: 99%

Design of microfluidic experimental setup for the detection of heavy metal ions using piezoresistive BioMEMS sensor

Rotake

Darji

Kale

2019

View full text Add to dashboard Cite

show abstract

“…In 2007, Yoo et al [ 154 ] modified an avidin-sensitive membrane on the surface of a microcantilever by self-assembly and monitored in real time the binding process of a streptavidin ligand. In 2010, Wang et al [ 155 ] used a microcantilever modified by a platelet-derived growth factor (PDGF) aptamer probe to quantitatively study the effect of temperature on the binding process between the probe and PDGF. As shown in Figure 13 , in their subsequent research from 2013, they integrated a microcantilever array modified by a PDGF aptamer on the microfluidic chip, and built a plug-and-play detection platform with a DVD-ROM as the optical detection module, which can realize fast, low-cost, and parallel detection [ 156 ].…”

Section: Application Of Genetic-probe-modified Cantilevermentioning

confidence: 99%

A Genosensor Based on the Modification of a Microcantilever: A Review

et al. 2023

View full text Add to dashboard Cite

When the free end of a microcantilever is modified by a genetic probe, this sensor can be used for a wider range of applications, such as for chemical analysis, biological testing, pharmaceutical screening, and environmental monitoring. In this paper, to clarify the preparation and detection process of a microcantilever sensor with genetic probe modification, the core procedures, such as probe immobilization, complementary hybridization, and signal extraction and processing, are combined and compared. Then, to reveal the microcantilever’s detection mechanism and analysis, the influencing factors of testing results, the theoretical research, including the deflection principle, the establishment and verification of a detection model, as well as environmental influencing factors are summarized. Next, to demonstrate the application results of the genetic-probe-modified sensors, based on the classification of detection targets, the application status of other substances except nucleic acid, virus, bacteria and cells is not introduced. Finally, by enumerating the application results of a genetic-probe-modified microcantilever combined with a microfluidic chip, the future development direction of this technology is surveyed. It is hoped that this review will contribute to the future design of a genetic-probe-modified microcantilever, with further exploration of the sensitive mechanism, optimization of the design and processing methods, expansion of the application fields, and promotion of practical application.

show abstract

“…Microcantilevers functionalised with metal‐binding protein ‘AgNt84‐6’, are demonstrated for the detection of HMIs like Hg 2+ and Zn 2+ by Cherian et al [44]; SAMs (self‐assembled monolayer's) modified microcantilevers used for detection of Ca 2+ ions by Ji and Thundat [45]; arrays of microcantilever sensors encapsulated in fluidic wells by Venstra et al [46] and fluidic channel by Xiang and Lee [47]; A chitosan (CS)‐graphene oxide (GO) surface plasmon resonance (SPR) sensor by Kamaruddin et al [48]. A microcantilever/diaphragm‐based system [49–52], but all of these methods use optical readout, which is a cumbersome method and required costly lab equipment. The optical readout has some disadvantages when used with microcantilever‐based biosensors in the microfluidic environment when the refractive index of liquid changes and when used in opaque liquid because it absorbs the laser light by Thaysen [53].…”

Section: Introductionmentioning

confidence: 99%

Highly selective sensor for the detection of Hg ²⁺ ions using homocysteine functionalised quartz crystal microbalance with cross‐linked pyridinedicarboxylic acid

Rotake

Kumar

Darji

et al. 2020

IET nanobiotechnol.

View full text Add to dashboard Cite

Microcantilever-based label-free thermal characterization of biomolecular affinity binding

Cited by 6 publications

References 7 publications

Design of microfluidic experimental setup for the detection of heavy metal ions using piezoresistive BioMEMS sensor

Design of microfluidic experimental setup for the detection of heavy metal ions using piezoresistive BioMEMS sensor

A Genosensor Based on the Modification of a Microcantilever: A Review

Highly selective sensor for the detection of Hg ²⁺ ions using homocysteine functionalised quartz crystal microbalance with cross‐linked pyridinedicarboxylic acid

Contact Info

Product

Resources

About

Microcantilever-based label-free thermal characterization of biomolecular affinity binding

Cited by 6 publications

References 7 publications

Design of microfluidic experimental setup for the detection of heavy metal ions using piezoresistive BioMEMS sensor

Design of microfluidic experimental setup for the detection of heavy metal ions using piezoresistive BioMEMS sensor

A Genosensor Based on the Modification of a Microcantilever: A Review

Highly selective sensor for the detection of Hg 2+ ions using homocysteine functionalised quartz crystal microbalance with cross‐linked pyridinedicarboxylic acid

Contact Info

Product

Resources

About

Highly selective sensor for the detection of Hg ²⁺ ions using homocysteine functionalised quartz crystal microbalance with cross‐linked pyridinedicarboxylic acid