Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh

Abstract

Department of Food and Life Science, Sungkyunkwan University, Chunchun-dong 300, Jangan-gu, Suwon, Korea A Gram-negative, non-motile, non-spore-forming, moderately halophilic rod (strain SM-1 T ) was isolated from salt marsh around the junction of the Youngsan River and the Yellow Sea in Korea and subjected to a polyphasic taxonomic study. This organism grew optimally at 37˚C and was able to grow at 10 and 45˚C. It grew optimally in the presence of 2-3 % (w/v) NaCl. The major fatty acids in strain SM-1 T were iso-C 15 : 0 and C 16 : 0 . Strain SM-1 T and Microbulbifer hydrolyticus DSM 11525 T were characterized by having ubiquinone-8 as the predominant respiratory lipoquinone. The DNA G+C content of strain SM-1 T was 59 mol%. Phylogenetic analysis based on 16S rDNA sequences showed that strain SM-1 T formed a coherent cluster with M. hydrolyticus; this relationship was supported by a bootstrap resampling value of 100 %. The level of 16S rDNA identity between strain SM-1 T and the type strain of M. hydrolyticus was 98?6 %. The mean level of DNA-DNA relatedness between strain SM-1 T and the type strain of M. hydrolyticus was 20?6 %. Therefore, on the basis of phenotypic properties, phylogeny and genomic data, strain SM-1 T should be placed in the genus Microbulbifer as a member of a novel species, for which the name Microbulbifer salipaludis sp. nov. is proposed. The type strain of the novel species is strain SM-1 T (5KCCM 41586 T 5JCM 11542 T ).

Microbulbifer maritimus sp. nov., isolated from an intertidal sediment from the Yellow Sea, Korea

“…A single species, Microbulbifer hydrolyticus, was included within this genus. The second species of the genus Microbulbifer was Microbulbifer salipaludis, which was proposed recently by Yoon et al (2003b). Phylogenetic analyses based on 16S rDNA sequences have shown that the two Microbulbifer species fall within the c-subclass of the Proteobacteria (González et al, 1997; Solano & Sanchez-Amat, 1999;Anzai et al, 2000;Yoon et al, 2003b).…”

“…The second species of the genus Microbulbifer was Microbulbifer salipaludis, which was proposed recently by Yoon et al (2003b). Phylogenetic analyses based on 16S rDNA sequences have shown that the two Microbulbifer species fall within the c-subclass of the Proteobacteria (González et al, 1997; Solano & Sanchez-Amat, 1999;Anzai et al, 2000;Yoon et al, 2003b). Chemical markers of this genus include the presence of a major amount of iso-C 15 : 0 and ubiquinone with eight isoprene units (Q-8) (González et al, 1997;Yoon et al, 2003b).…”

“…Phylogenetic analyses based on 16S rDNA sequences have shown that the two Microbulbifer species fall within the c-subclass of the Proteobacteria (González et al, 1997; Solano & Sanchez-Amat, 1999;Anzai et al, 2000;Yoon et al, 2003b). Chemical markers of this genus include the presence of a major amount of iso-C 15 : 0 and ubiquinone with eight isoprene units (Q-8) (González et al, 1997;Yoon et al, 2003b). The DNA G+C content of the genus is 58-59 mol% (González et al, 1997;Yoon et al, 2003b).…”

Gramella sediminilitoris sp. nov., isolated from a tidal flat

“…The 16S rRNA gene was amplified by PCR as described previously (Yoon et al, 1998) using two universal primers, 9F (5¢-GAGTTTGATCCTGGCTCAG-3¢) and 1512R (5¢-ACGGTTACCTTGTTACGACTT-3¢). Sequencing of the amplified 16S rRNA gene and phylogenetic analysis were performed as described by Yoon et al (2003). DNA-DNA hybridization was performed fluorometrically by the method of Ezaki et al (1989) using photobiotinlabelled DNA probes in microdilution wells.…”

Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the Yellow Sea, Korea

“…Sequencing of the amplified 16S rRNA gene and phylogenetic analysis were performed as described by Yoon et al (2003). DNA-DNA hybridization was performed fluorometrically by the method of Ezaki et al (1989) using photobiotin-labelled DNA probes and microdilution wells.…”