Microbiomes of colored dental biofilms in children with or without severe caries experience

Abstract

Background Biofilm coloration can compromise maturation and increase the risk of oral disease in adulthood, though children with colored biofilm do not always demonstrate a poor oral health status. Aim The microbial compositions of colored and white biofilms in children were compared. Design Thirty‐two dental biofilm samples from 16 children (age < 13 years) were analyzed using 16S rRNA pyrosequencing, then the subjects were divided into severe caries and healthy (caries‐free) groups. Correlations between microbiomes and oral health status were also examined. Results Phylogenetic analysis revealed no distinctly different patterns between colored and white biofilms. In the severe caries group, genus Actinomyces, Cardiobacterium, Kingella, Lautropia, and Veillonella, and family Neisseriaceae were detected, though abundance was significantly different between colored and white biofilm specimens, in contrast to the healthy group. In addition, five colored biofilm samples from the severe caries group contained greater than 15% Actinomyces, which led us to consider that genus to be possibly associated with formation of colored biofilm in children. Conclusions Our findings indicate that differences in bacterial composition between colored and white biofilms are higher in individuals with severe caries. Additional research may reveal the significance of colored dental biofilm in children.

Deciphering Black Extrinsic Tooth Stain Composition in Children Using Metaproteomics

“…dentocariosa, and Rothia aeria, harbored a catechol-siderophore-like BGC, which is associated with BS, due to the toxicity of the catechol molecule, − (ii) Neisseria genus was described to be significantly different “highly abundant” in the BS category compared to the DP/control category; various species at the genus level including Neisseria and Streptococcus may be main contributors for the formation of BS. It was suggested that alterations in oral microbiome are crucial, (iii) Kingella genus was shown/detected to be significantly increased in severe caries group versus non-severe caries group, (iv) Pseudopropionibacterium genus has been reported to be linked to dental plaque disease as described by Siqueirajr and Rocas . Microorganisms were found in all cases of root-filled teeth linked to periradicular lesions that strongly support to the assertion that treatment failures are rather of infectious etiology, caused by persistent or secondary intraradicular infections.…”

Correlation between caries activity and salivary microbiota in preschool children

“…Campylobacter is associated with intestinal diseases, and its detection rate in the intestinal tract of patients with Inflammatory Bowel Disease (IBD) was higher than that of healthy people ( Qi et al., 2021 ). Campylobacter and Selenomonas used organic acids as energy sources and were closely associated with dental caries ( Aas et al., 2008 ; Nagai et al., 2020 ). In contrast, Campylobacter reportedly was more abundant in the oral cavity of healthy individuals than caries patients, and that increased abundance reduced the risk of caries ( Lif Holgerson et al., 2015 ; Xu et al., 2021a ).…”

The Microbiota Profile Analysis of Combined Periodontal-Endodontic Lesions Using 16S rRNA Next-Generation Sequencing

“…Kingella is a normal flora in the oral cavity, being detected in both healthy and periodontitis subjects [33,34]. To note, a study showed that Kingella was rich in biofilm collected from children with severe caries, indicating that Kingella might associate with invasion and erosion of enamel and dentin [35]. We speculate that predominate Kingella can degrade mineral substances and form the channel through which microbiota and secreted toxins can affect dental pulp.…”