Microbiome Affects the Adaptation of Drosophila melanogaster to a High NaCl Concentration

Ivnitsky, S. B.; Maximova, Irina A.; Панченко, П. Л.; Dmitrieva, A. S.; Качалкин, А. В.; Kornilova, M. B.; Perfilieva, K. S.; Марков, А. В.

doi:10.1134/s2079086419050049

Cited by 4 publications

(12 citation statements)

References 41 publications

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“…Next, we confirmed that pre-inoculation of the high salt medium with homogenized salt-tolerant flies (Fs1, Fs2) improved the reproduction of control (naïve) flies on this medium compared to pre-inoculation by homogenized flies from the control lines (Fn1, Fn2). Thus we show that the results obtained previously [43, 44] are replicable in four other fly lines, reared in different conditions (see above).…”

Section: Introductionsupporting

confidence: 85%

“…We obtained this result in four laboratory lines (two salt-tolerant and two control ones) reared in population cages with overlapping generations. We also found remarkable differences in the abundance and taxonomic composition of yeasts between salt-tolerant and control Drosophila homogenates [43, 44]. These results agree with the assumption that the increased tolerance of D .…”

Section: Introductionsupporting

confidence: 83%

“…melanogaster on food S. This result was obtained from the four D . melanogaster lines reared under different conditions (in population cages, with overlapping generations) [43, 44]. This apparently indicates that some quantitative or qualitative characteristics of the microbiome of the salt-tolerant flies, along with other features of the salt-tolerant fly homogenate, improve the reproductive efficiency of the flies on salty food.…”

Section: Discussionmentioning

confidence: 99%

“…We used the same testing technique previously in the course of our experiment [25–27, 43, 44]. We performed three tests on the reproductive efficiency (sections 1, 2 and 4 of the Results); 5 to 10 test vials were used for each fly line/substrate combination.…”

Section: Methodsmentioning

confidence: 99%

“…We show that there are robust differences between yeast communities from salt-tolerant and control fly lines. Most importantly, one yeast species, Starmerella bacillaris , is abundant in all salt-tolerant lines tested to date (Fs1, Fs2, and two other salt-tolerant lines tested in [44]) and absent or very scarce in all control lines (Fn1, Fn2, and two other control lines tested in [44]).…”

Section: Introductionmentioning

confidence: 99%

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Yeasts affect tolerance of Drosophila melanogaster to food substrate with high NaCl concentration

et al. 2019

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The ability of model animal species, such as Drosophila melanogaster, to adapt quickly to various adverse conditions has been shown in many experimental evolution studies. It is usually assumed by default that such adaptation is due to changes in the gene pool of the studied population of macroorganisms. At the same time, it is known that microbiome can influence biological processes in macroorganisms. In order to assess the possible impact of microbiome on adaptation, we performed an evolutionary experiment in which some D. melanogaster lines were reared on a food substrate with high NaCl concentration while the others were reared on the standard (favourable) substrate. We evaluated the reproductive efficiency of experimental lines on the high salt substrate three years after the experiment started. Our tests confirmed that the lines reared on the salty substrate became more tolerant to high NaCl concentration. Moreover, we found that pre-inoculation of the high salt medium with homogenized salt-tolerant flies tended to improve reproductive efficiency of naïve flies on this medium (compared to pre-inoculation with homogenized control flies). The analysis of yeast microbiome in fly homogenates revealed significant differences in number and species richness of yeasts between salt-tolerant and control lines. We also found that some individual yeast lines extracted from the salt-tolerant flies improved reproductive efficiency of naïve flies on salty substrate (compared to baker’s yeast and no yeast controls), whereas the effect of the yeast lines extracted from the control flies tended to be smaller. The yeast Starmerella bacillaris extracted from the salt-tolerant flies showed the strongest positive effect. This yeast is abundant in all salt-tolerant lines, and very rare or absent in all control lines. The results are consistent with the hypothesis that some components of the yeast microbiome of D. melanogaster contribute to to flies’ tolerance to food substrate with high NaCl concentration.

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Section: Introductionsupporting

confidence: 85%

Section: Introductionsupporting

confidence: 83%