Age-Related Changes in the Yeast Component of the Drosophila melanogaster Microbiome

Dmitrieva, A. S.; Maksimova, I. A.; Качалкин, А. В.; Марков, А. В.

doi:10.1134/s0026261721020028

Cited by 5 publications

(8 citation statements)

References 41 publications

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“…This aligns with the observation that, with the increase in salt concentration, the percentage of Acetobacter (which can be beneficial for flies) decreased, while the proportion of lactic acid bacteria (also potentially helpful) first increased (from 0% to 4% NaCl), and then drastically decreased at 7% NaCl. It is also in line with the presence of Starmerella yeasts in the fly microbiome at high NaCl concentrations, previously shown to help flies survive and reproduce on the salty substrate [42,44] . These yeasts may also assist the flies in resisting potentially pathogenic bacteria, such as Leuconostoc and Providencia, whose percentage increased at higher salinity.…”

Section: Discussionsupporting

confidence: 83%

“…The absence of S. bacillaris in lines 4a-4c and the presence of this yeast in lines 7a-7b are noteworthy. In 2017-2018, S. bacillaris dominated in lines 4a-4c [41,42] , but later, in 2019-2020 and the current study, it was no longer found in them. This change likely represents coevolutionary dynamics or different stages of adaptation of the holobiont (the insect host and its associated microorganisms) to the salty substrate.…”

Section: Discussionsupporting

confidence: 39%

“…It can be hypothesized that the yeast S. bacillaris tends to develop in fly lines that have recently been switched to a saltier substrate (like lines 7a-7b) and have not yet adapted genetically. The yeasts may help the flies tolerate high NaCl concentrations at this early adaptation stage, as shown in our previous studies [42] . However, later, these beneficial symbionts may be lost by flies [43] as the insects gradually develop genetic adaptations to the salty substrate.…”

Section: Discussionmentioning

confidence: 56%

“…Previous studies have demonstrated that the abundance and species composition of yeasts associated with D. melanogaster may depend on the salt concentration in the substrate. Certain yeast species, such as S. bacillaris, may contribute to the adaptation of laboratory fly lines to salty substrates [41][42][43][44] . Evidently, the yeast community of flies adapts to the salty substrate alongside the host organism, reflecting the adaptability of the entire symbiotic complex.…”

Section: Introductionmentioning

confidence: 99%

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Salt concentration in substrate modulates the composition of bacterial and yeast microbiomes of Drosophila melanogaster

Yakovleva,

Danilova,

Maximova

et al. 2024

Microbiome Res Rep

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Aim: Microbiomes influence the physiology and behavior of multicellular organisms and contribute to their adaptation to changing environmental conditions. However, yeast and bacterial microbiota have usually been studied separately; therefore, the interaction between bacterial and yeast communities in the gut of Drosophila melanogaster (D. melanogaster ) is often overlooked. In this study, we investigate the correlation between bacterial and yeast communities in the gut of D. melanogaster . Methods: We studied the shifts in the joint microbiome of Drosophila melanogaster , encompassing both yeasts and bacteria, during adaptation to substrate with varying salt concentrations (0%, 2%, 4%, and 7%) using plating for both yeasts and bacteria and NGS-sequencing of variable 16S rRNA gene regions for bacteria. Results: The microbiome of flies and their substrates was gradually altered at moderate NaCl concentrations (2% and 4% compared with the 0% control) and completely transformed at high salt concentrations (7%). The relative abundance of Acetobacter , potentially beneficial to D. melanogaster , decreased as NaCl concentration increased, whereas the relative abundance of the more halotolerant lactobacilli first increased, peaking at 4% NaCl, and then declined dramatically at 7%. At this salinity level, potentially pathogenic bacteria of the genera Leuconostoc and Providencia were dominant. The yeast microbiome of D. melanogaster also undergoes significant changes with an increase in salt concentration in the substrate. The total yeast abundance undergoes nonlinear changes: it is lowest at 0% salt concentration and highest at 2%-4%. At a 7% concentration, the yeast abundance in flies and their substrate is lower than at 2%-4% but significantly higher than at 0%. Conclusions: The abundance and diversity of bacteria that are potentially beneficial to the flies decreased, while the proportion of potential pathogens, Leuconostoc and Providencia , increased with an increase in salt concentration in the substrate. In samples with a relatively high abundance and/or diversity of yeasts, the corresponding indicators for bacteria were often lowered, and vice versa . This may be due to the greater halotolerance of yeasts compared to bacteria and may also indicate antagonism between these groups of microorganisms.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 39%

Section: Discussionmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Salt concentration in substrate modulates the composition of bacterial and yeast microbiomes of Drosophila melanogaster

Yakovleva,

Danilova,

Maximova

et al. 2024

Microbiome Res Rep

View full text Add to dashboard Cite

show abstract

“…Several studies reported that microorganisms associated with Drosophila larvae can be detected in young adults, a phenomenon called transstadial maintenance or maintenance through metamorphosis. 24 , 25 , 28 , 51 , 52 In the wild, environmental transmission between the larval and adult stages is unlikely, because Drosophila juveniles usually leave larval substrates to pupate elsewhere, often in soil. 53 , 54 We mimicked this behavior in an additional experiment to confirm that yeasts associated with Drosophila larvae maintained until adult emergence under ecologically realistic conditions.…”

Section: Resultsmentioning

confidence: 99%