Changes in the Symbiotic Yeast of Drosophila melanogaster during Adaptation to Substrates with an Increased NaCl Content

Dmitrieva, A. S.; Yakovleva, Ekaterina; Maksimova, I. A.; Belov, A. A.; Марков, А. В.

doi:10.1134/s2079086423010036

Cited by 2 publications

(11 citation statements)

References 27 publications

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“…The yeasts may help the flies tolerate high NaCl concentrations at this early adaptation stage, as shown in our previous studies [42] . However, later, these beneficial symbionts may be lost by flies [43] as the insects gradually develop genetic adaptations to the salty substrate. Further experiments are needed to test this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

“…Two lines labeled 7a and 7b were reared on a 4% NaCl medium until 2016, and then the salt concentration was increased by 0.5% every 2-3 months until it reached 7%. After that, 7a and 7b lines reared on medium with 7% NaCl medium as described previously [41,43,47] . The fly lines were reared in cylindrical glass jars 0.25 L (0c, 2a-2c, and 4a-4c) or population cages 165 mm × 165 mm × 250 mm (0a-0b, 7a-7b) with medium and water.…”

Section: Samples Selection and Preparationmentioning

confidence: 99%

“…Thirty adult flies at the age of 7 days after the eclosion were taken from each fly line. This age of flies was chosen not by chance: previously, it was shown that flies emerged from the pupa being almost sterile [2] and formed a microbiome typical for their line by the age of 7 days by eating previously processed substrate [43] . To remove the substrate particles and the microbial cells from the surface of the body, the flies were washed in 10 mL of sterile water on a MultiReax vortex (Heidolph, Germany) at 1,700 rpm for 15 min.…”

Section: Samples Selection and Preparationmentioning

confidence: 99%

“…Previous studies have demonstrated that the abundance and species composition of yeasts associated with D. melanogaster may depend on the salt concentration in the substrate. Certain yeast species, such as S. bacillaris, may contribute to the adaptation of laboratory fly lines to salty substrates [41][42][43][44] . Evidently, the yeast community of flies adapts to the salty substrate alongside the host organism, reflecting the adaptability of the entire symbiotic complex.…”

Section: Introductionmentioning

confidence: 99%

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Salt concentration in substrate modulates the composition of bacterial and yeast microbiomes of Drosophila melanogaster

Yakovleva,

Danilova,

Maximova

et al. 2024

Microbiome Res Rep

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Aim: Microbiomes influence the physiology and behavior of multicellular organisms and contribute to their adaptation to changing environmental conditions. However, yeast and bacterial microbiota have usually been studied separately; therefore, the interaction between bacterial and yeast communities in the gut of Drosophila melanogaster (D. melanogaster ) is often overlooked. In this study, we investigate the correlation between bacterial and yeast communities in the gut of D. melanogaster . Methods: We studied the shifts in the joint microbiome of Drosophila melanogaster , encompassing both yeasts and bacteria, during adaptation to substrate with varying salt concentrations (0%, 2%, 4%, and 7%) using plating for both yeasts and bacteria and NGS-sequencing of variable 16S rRNA gene regions for bacteria. Results: The microbiome of flies and their substrates was gradually altered at moderate NaCl concentrations (2% and 4% compared with the 0% control) and completely transformed at high salt concentrations (7%). The relative abundance of Acetobacter , potentially beneficial to D. melanogaster , decreased as NaCl concentration increased, whereas the relative abundance of the more halotolerant lactobacilli first increased, peaking at 4% NaCl, and then declined dramatically at 7%. At this salinity level, potentially pathogenic bacteria of the genera Leuconostoc and Providencia were dominant. The yeast microbiome of D. melanogaster also undergoes significant changes with an increase in salt concentration in the substrate. The total yeast abundance undergoes nonlinear changes: it is lowest at 0% salt concentration and highest at 2%-4%. At a 7% concentration, the yeast abundance in flies and their substrate is lower than at 2%-4% but significantly higher than at 0%. Conclusions: The abundance and diversity of bacteria that are potentially beneficial to the flies decreased, while the proportion of potential pathogens, Leuconostoc and Providencia , increased with an increase in salt concentration in the substrate. In samples with a relatively high abundance and/or diversity of yeasts, the corresponding indicators for bacteria were often lowered, and vice versa . This may be due to the greater halotolerance of yeasts compared to bacteria and may also indicate antagonism between these groups of microorganisms.

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Section: Discussionmentioning

confidence: 99%

Section: Samples Selection and Preparationmentioning

confidence: 99%

Section: Samples Selection and Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Salt concentration in substrate modulates the composition of bacterial and yeast microbiomes of Drosophila melanogaster

Yakovleva,

Danilova,

Maximova

et al. 2024

Microbiome Res Rep

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“…Some insects, such as ants, termites, and beetles, cultivate fungi as their food source ( Beaver, 1989 ; Mueller et al., 1998 ; Aanen et al., 2002 ). Yeasts like Starmerella bacillaris , St. etchellsii , Candida californica , Pichia membranifaciens , P. occidentalis , and Zygosaccharomyces bailii have been identified as beneficial symbionts in Drosophilla melanogaster microbiome ( Dmitrieva et al., 2023 ). Fall armyworm ( Spodoptera frugiperda ) harbors fungal symbionts such as Fusarium oxysporum and Cladosporium sp ( Watson et al., 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Distinct fungal microbiomes of two Thai commercial stingless bee species, Lepidotrigona terminata and Tetragonula pagdeni suggest a possible niche separation in a shared habitat

Castillo,

Sinpoo,

Phokasem

et al. 2024

Front. Cell. Infect. Microbiol.

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Stingless bees, a social corbiculate bee member, play a crucial role in providing pollination services. Despite their importance, the structure of their microbiome, particularly the fungal communities, remains poorly understood. This study presents an initial characterization of the fungal community associated with two Thai commercial stingless bee species, Lepidotrigona terminata (Smith) and Tetragonula pagdeni (Schwarz) from Chiang Mai, Thailand. Utilizing ITS amplicon sequencing, we identified distinct fungal microbiomes in these two species. Notably, fungi from the phyla Ascomycota, Basidiomycota, Mucoromycota, Mortierellomycota, and Rozellomycota were present. The most dominant genera, which varied significantly between species, included Candida and Starmerella. Additionally, several key enzymes associated with energy metabolism, structural strength, and host defense reactions, such as adenosine triphosphatase, alcohol dehydrogenase, β-glucosidase, chitinase, and peptidylprolyl isomerase, were predicted. Our findings not only augment the limited knowledge of the fungal microbiome in Thai commercial stingless bees but also provide insights for their sustainable management through understanding their microbiome.

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Changes in the Symbiotic Yeast of Drosophila melanogaster during Adaptation to Substrates with an Increased NaCl Content

Cited by 2 publications

References 27 publications

Salt concentration in substrate modulates the composition of bacterial and yeast microbiomes of Drosophila melanogaster

Salt concentration in substrate modulates the composition of bacterial and yeast microbiomes of Drosophila melanogaster

Distinct fungal microbiomes of two Thai commercial stingless bee species, Lepidotrigona terminata and Tetragonula pagdeni suggest a possible niche separation in a shared habitat

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