Microbiological Characteristics and Pathogenesis of Klebsiella pneumoniae Isolated from Hainan Black Goat

Abstract

K. pneumoniae is an opportunistic pathogen that leads to widespread infection in humans and animals, seriously threatening human health and animal husbandry development. In our research, we investigated the biological characteristics of the isolate by using a 16S rRNA gene sequencing, biochemical assay, and drug sensitivity test. Moreover, the pathogenicity study, including the bacteria load determination, the histopathology examination, and the RNA sequencing was carried out to explore whether the isolate could cause lung injury in mice through intraperitoneal injection. The results indicated that the isolate was identified as K. pneumoniae and named as KPHN001. The drug susceptibility test showed that KPHN001 was only sensitive to polymyxin B and furazolidone, and was resistant to other 28 antibiotics. In the bacteria load determination, the highest bacterial load of the organs was found in the spleen, and abundant bacterial colonization was also found in the lung. The histopathology showed the mainly acute inflammations in the lung were due to congestion, edema, and exudation. RNA-seq analysis revealed that the differentially expressed genes (DEGs) of inflammatory cytokines and chemokines were expressed massively in mice. In the present research, the biological characteristics and pathogenesis of clinically isolated K. pneumoniae were systematically studied, revealing the pathogenic mechanism of K. pneumoniae to animals, and providing a theoretical basis for the following prevention, control, and diagnosis research.

Identification and characterization of klebsiella pneumonia isolated from farm animals and their biofilm production estimation

“…In current study, The samples were examined for presence of klebsiella isolates using bacteriological methods, the obtained isolation rate are nearly similar to that recoded by Chiaverini et al (2022) who examined samples from wild animals. In addition, He et al (2022) detected the same cultural characters of klebsiella as and recorded that the isolated bacteria were cultured on TSA, blood agar, and McConkey agar for 24 h and grew well. Large, regular, round, smooth, raised, moist, and cream yellow colonies grew on TSA.…”

MALDI-TOF MS and MICRONAUT susceptibility test as recent bacteriological applications of Klebsiella spp isolated from large animals in Egypt.

“…Thirteen K. pneumoniae isolates (19.1%) exhibited acquired resistance to several antibiotic classes, including tetracyclines, aminoglycosides, and -lactams. (He et al, 2022)reported that KPHN001 was only sensitive to polymyxin B and furazolidone and was resistant to penicillin, cefoperazone, ofloxacin, gentamicin, kanamycin, and 28 other antibiotics. Likewise, (Tartor et al, 2021)demonstrated that K. pneumoniae was resistant to cephalothin, cefazolin, cefoxitin, ampicillin, amoxicillinclavulanic acid, and bacterial protein analysis.…”

Prevalence of multidrug resistance and extended-spectrum beta-lactamase-producing Klebsiella pneumoniae from dairy cattle farm wastewater in East Java Province, Indonesia

“…Klebsiella pneumoniae forms mucoid colonies on the media and has a large polysaccharide capsule [18]. Gram-stain results showed that the isolate was Gram-negative (Figure -2c) [29]. Pure colonies suspected of being K. pneumoniae on MCA media were plated on EMBA media.…”