Identification and characterization of klebsiella pneumonia isolated from farm animals and their biofilm production estimation

Abstract

Klebsiella pneumoniae considered one of the most commensal bacteria which cause Economical problems in farm animals as causing mastitis and respiratory disorders in ruminants and neonatal septicemia in calves. Antibiotic are commonly used for disease control in animals and human. Multidrug resistant (MDR) K. pneumoniae strain show resistance to more than one antibiotic. In the present study K. pneumoniae were isolated and identified from different clinical samples (nasal swabs, milk sample, anal and vaginal swabs). A total of 293 samples from 258 animals (190 sample from clinically diseased cattle, 60 from clinically diseased buffaloes and 43 from clinically diseased calves). Results showed that the isolation rates were 20 /190 samples taken from clinically diseased cattle(10.5%), 5 /60 samples from clinically diseased buffaloes (8.33%) and 5 /43 samples from calfs (11.6%). Klebsiella was isolated between samples type as 24 /233 nasal swabs, 4/45 mastitic milk sample and 2 /10 anal swabs sample with percentage of 10.3%, 8.8, and 20%, respectivly. The anti-microbial susceptability test were done for 30 isolates of klebsiella using disc diffusion method. The results showed that the highest resistance was to ampicillin (100%), followed by chloramohinicol (76.7%) and the highest sensitivity was to gentamycin (100%), followed by ciprofluxacin and erythromycin (76.7%), nalidexic (73.33%), and streptomycin (53.4%). The biofilm formation ability of k. pneumoniae protects bacteria from immune system of the host. The present study of biofim formation module indicated that all strains were biofilm formed strains and among them, 11 (36.66%), 12 (40%), and 7 (23,33%) were strong, moderate, and weak biofilm producers, respectively. In conclusion, the detection of MDR klebsiella pneumoni from animals the isolates showed different ability for biofilm formation Klebsiella pneumoniaMultidrug resistant (MDR) biofilm formation