Microbiological and parasitological quality of honey produced in İstanbul

Dümen, Emek; Akkaya, Hayrettin; Öz, Gülay Merve; Sezgin, Funda Hatice

doi:10.3906/vet-1301-46

Cited by 20 publications

(18 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite its richness in sugar and inhibins, honey cannot be considered as sterile since many studies proved that it is subject to bacterial and fungal contaminations derived from two kind of sources: primary and secondary [9],[10],[11]. The first ones include pollen, dirt, dust, air, water, flowers, as well as the digestive tracts of honeybees and are considered as natural sources which are difficult to control [12] [13]. The secondary sources are those arising from the honey manipulation by people, thus are closely connected with hygiene of processing, handling, and storage [11].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of bacteria associated with honeys of different geographical and botanical origin using two different identification approaches: MALDI-TOF MS and 16S rDNA PCR technique

et al. 2019

View full text Add to dashboard Cite

In the presented work identification of microorganisms isolated from various types of honeys was performed. Martix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rDNA sequencing were applied to study environmental bacteria strains.With both approches, problematic spore-forming Bacillus spp , but also Staphylococcus spp ., Lysinibacillus spp ., Micrococcus spp . and Brevibacillus spp were identified. However, application of spectrometric technique allows for an unambiguous distinction between species/species groups e.g. B . subtilis or B . cereus groups. MALDI TOF MS and 16S rDNA sequencing allow for construction of phyloproteomic and phylogenetic trees of identified bacterial species. Furthermore, the correlation beetween physicochemical properties, geographical and botanical origin and the presence bacterial species in honey samples were investigated.

show abstract

Section: Introductionmentioning

confidence: 99%

“…and Bacillus spp. [16], [17], [13]. Although studies on microbial contamintation of honey are mainly focused on the occurrence of C .…”

Section: Introductionmentioning

confidence: 99%

Analysis of bacteria associated with honeys of different geographical and botanical origin using two different identification approaches: MALDI-TOF MS and 16S rDNA PCR technique

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The results coincide with those obtained by other authors. Gomes et al (2010) isolated Salmonella spp., Coliforms and E. coli in Portugal at a 34% rate; Kokubo et al (1984) analyzed 70 samples of honey and isolated Dumen et al (2013) studied the honey produced in Istanbul and verified the presence of coliforms in 18% of 80 samples.…”

Section: Resultsmentioning

confidence: 99%

Quality of honey bees Apis mellifera sold in the state of Alagoas, Brasil

Alves¹,

Santos²,

Neto³

et al. 2015

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

The present study aimed to determine the quality of honey marketed in the State of Alagoas, Brazil. Fifteen samples of Apis mellifera L. honey sold in supermarkets, free trade, and cooperative located in the State of Alagoas were acquired. Microbiological and physical-chemical analyzes were carried out to establish a standard microbiology condition and check for possible tampering. The physico-chemical analyzes showed that all the samples studied presented acid pH values ranging between 2.3 and 4.4. For diastase activity and reaction, Lugol which are indicative of the presence of starch and dextrin, and reaction Fiehe, which is a qualitative indicator of HMF, all samples were negative for at least the parameters. As the microbiological standard, 26.6% of all samples showed high standard count mesophilic aerobic bacteria, 20% had counts of molds and yeasts above the quality standards established by Brazilian law. For enumeration of coliforms at 35 and 45°C, it was found that most samples were contaminated (86.7%). It is the presence of sporulated bacteria in 13.3% of the samples, which were 15.26 and 84.64% genus Clostridium of the genus Bacillus.

show abstract

“…Honey also has several sources of microbiological contamination such as bacteria, fungi, and parasites such as microsporidia. The primary sources of this contamination include plant materials such as pollen and nectarand abiotic environmental material such as dust and soil [7]. In addition, entomopathogenic bacteria used forbiological control of plant pests can also infect honeybees when they are applied to fields.…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity of aerobic bacteria isolated from honeybees (Apis mellifera) in Ordu Province

Boğ¹,

Ertürk²,

Yaman³

2020

Turk J Vet Anim Sci

View full text Add to dashboard Cite

of the honeybee and test their insecticidal effects on adult honeybees. 2. Materials and methods 2.1. Insect samples Samples of honeybees were collected from bee farms in the vicinity of Ordu,Turkey from May to September.The adult bees collected from the hives were placed in plastic boxes (20 × 20 cm) with punched lids for ventilation, and sugar cake was given in small pieces until the bees were transported to the laboratory for examination. Following this, healthy, diseased, and dead adult bees were separated for observation and used to isolate bacteria that could cause disease. 2.2. Isolation of bacteria Isolation and purification of bacteria was carried out individually from dead and live honeybees. The live and dead adults were individually surface sterilized by using 70% ethanol for 3 min [8] and washed 3 times with sterile water. The dead honeybee carcasses were homogenized in feeder medium using a glass tissue mill, and 2 layers Abstract: The honeybee (Apis mellifera) is an important pollinator insect. Any pathogenic infection in this beneficial insect is undesirable. In this study, bacterial diversity in beehives was investigated to determine the potential of pathogenic bacteria in honeybees. To do this, bacterial isolations were carried out from dead and diseased adult bees collected from 9 districts in Ordu Province in Turkey.Twenty species of pathogenic bacteria, 18 of which were nonsporeforming Staphylococcus lentus, Klebsiella oxytoca, Citrobacter freundii, Leucanostoc mesenteroides ssp. cremoris, Kocuria rosea, Kocuria kristinae, Sphingomonas paucimobilis slashline, Burkholderia cepacia, Leucanostoc mesenteroides ssp. dextranicum,Hafnia alvei, Escherichia coli, Aeromonas salmonicida, Citrobacter braakii, Pantoea agglomerans, Streptococcus equi ssp. zooepidemicus, Staphylococcus pseudintermedius, Staphylococcus lugdunesis and Staphylococcus vitulinus and 2 sporeforming Bacillus licheniformis and Paenibacillus polymyxa, were isolated and identified from the honeybees. The infectivity of these bacteria were also documented with bioassay experiments on the healthy bees.The highest insecticidal effectwas determinedwith Bacillus licheniformis (84%), Escherichia coli (84%), and Streptococcus equi ssp. zooepidemicus (80%) on the adult honeybees.This result confirms that the honeybee contains a very large number of bacterial species and that the majority of them are pathogenic for the species in Turkey. In addition, some of the entomopathogenic bacteria used for biological control can have negative impact on this economically important insect.

show abstract

Microbiological and parasitological quality of honey produced in İstanbul

Cited by 20 publications

References 26 publications

Analysis of bacteria associated with honeys of different geographical and botanical origin using two different identification approaches: MALDI-TOF MS and 16S rDNA PCR technique

Analysis of bacteria associated with honeys of different geographical and botanical origin using two different identification approaches: MALDI-TOF MS and 16S rDNA PCR technique

Quality of honey bees Apis mellifera sold in the state of Alagoas, Brasil

Pathogenicity of aerobic bacteria isolated from honeybees (Apis mellifera) in Ordu Province

Contact Info

Product

Resources

About