Microbial transformation of phytosterols mixture from rice bran oil unsaponifiable matter by selected bacteria

Abstract

Rice bran sample (12 Kg) was extracted and rice bran oil (RBO % 76.8 g) was saponified. The resulted unsaponifiable matter of RBO (RBO unsap) was qualitatively and quantitatively estimated using different chromatographic analyses. RBO, produced 9.65% unsaponifiable matter with the following contents, cholesterol, 6.75%; stigmasterol, 3.4%; b. sitosterol, 10.23% and campesterol, 4.2%, in addition to unknown phytosterols, hydrocarbons and waxes. Microbial transformation process started by screening of 35 bacterial strains, locally isolated from rice bran, air and soil, using RBO unsap as a carbon and an energy source to produce some pharmaceutically useful C 18 and C 19 steroids. Moraxella ovis was the most potent isolate for its highest capability to utilize RBO unsap and selectively degrade the phytosterols side-chain producing androst-4-ene-3,17-dione (AD), androsta-1,4-diene-3,17-dione (ADD), testosterone (T) and estrone (E). The RBO unsap was the best carbon and energy source. Maximum production of the desired products was observed in 36 h, pH 7 and at 30°C by M. ovis.

Single step biotransformation of corn oil phytosterols to boldenone by a newly isolated Pseudomonas aeruginosa

“…The incubation time is critical for steroid biotransformation. Maximum production of BOL in our study was achieved at 36 h (42.4%), on extending the fermentation process to 60 h, a remarkable reduction in BOL% was noticed, The same results were reported by others [7] , [29] . The results of these experiments indicated that AD and ADD production depended strongly on biotransformation time and substrate concentration [30] .…”

“…Optimum pH 8 was also reported by Goswami et al [31] for production of AD (D) from 17-ketosteroids by Arthrobacter . In addition, optimal pH between 6 and 8 for steroid biotransformation had referred by several reports as Sallam et al [7] and Zhang et al [25] ; they reported optimal pH at 7. Abd-Elsalam et al [30] reported optimum pH 6.5 for production of androstenedione (AD) and androstadienedione (ADD) from sunflower oil sterols by Fusarium solani .…”

“…Only microorganisms that can utilize corn oil as a sole carbon and energy source will grow and then isolated, purified and transferred to maintenance slants. Five bacterial strains were isolated and maintained on modified nutrient agar [7] at pH 6.8–7.2. The agar slopes were incubated for 24 h at 30 °C then stored at 4 °C with monthly subculture maintenance.…”

“…Detector temperature 295 °C as the hydrogen flow was 30 ml/min and air flow was 350 ml/min. Helium was the carrier gas at a flow rate of 30 ml/min [7] .…”

Microbial Biotransformation for the Production of Steroid Medicament

“…Several researchers have reported their work in developing a microbial biotransformation process with high yield of AD with wide industrial applications [9][10][11]. Therefore, it was important to understand the factors affecting the actual biotransformation process.…”