Microbial stabilization of antibiotic-containing urine samples by using the FLORA-STAT urine transport system

Abstract

The FLORA-STAT Urine Transport System (Wadley Biosciences Corp./Lymphokine Partners Ltd., Dallas, Tex.) was evaluated for its efficacy in maintaining organism count and in effectively blocking the bactericidal action of therapeutic antimicrobial agents in urine samples when the urine samples were held at room temperature. Reconstructions with 53 organism-antimicrobial combinations were performed at 0, 4, 8, and 24 h in which the FLORA-STAT system was compared with two boric acid-based systems (Urine C&S Transport Kit [Becton Dickinson VACUTAINER Systems, Rutherford, N.J.]; Sage Urine Collection Kit for Culture [Sage Products, Inc., Cary, Ill.]) and untreated urine. At 24 h, <1-log-unit changes in organism counts were found in 100, 92, and 10% of the urine samples without antimicrobial agents and in 97, 65, and 16% of the urine samples with antimicrobial agents for FLORA-STAT-treated, boric acid-treated, and untreated urine samples, respectively. The FLORA-STAT system was further evaluated by sending split samples prepared from laboratory-inoculated patient urine samples (57 without and 50 with antimicrobial agents) to four commercial laboratories by using their respective transport devices and procedures. Samples were also sent to a local reference laboratory which provided prompt processing. Each laboratory received independently labeled transport devices containing untreated, FLORA-STAT-treated, and preservative-treated (if provided by the commercial laboratory) samples prepared from the same urine specimen of a patient. Average estimated transport times ranged from 13 to 24 h for the commercial laboratories; the transport time was <4 h for the local reference laboratory. An average of 98, 94, and 53% of the antimicrobial agent-free FLORA-STATtreated, preservative-treated, and untreated samples, respectively, were maintained during transport. The FLORA-STAT system also maintained an average of 85% of the antimicrobial agent-containing samples, compared with 55 and 38% for the preservative-treated and untreated samples, respectively. Moreover, the FLORA-STAT system was not adversely affected by any specific class or type of antimicrobial agent.

Influence of the Transport on the Outcome of the Bacteriological Analysis of Dog Urine Comparison of Three Transport Tubes

“…Urine antibacterial activity is mainly due to antibiotic content of the specimen after antibiotherapy (12). Our results and those of DORN (7) with human urine samples show that the antibacterial activity of urine due to the antibiotics cannot be blocked by the boric acid preservative or by transport on the Urotube. This emphazises the need for assessment of the antimicrobial activity of the urine samples submitted to the laboratory, helping to correctly interpret the bacteriological findings.…”