Microbial sensors for naphthalene using Sphingomonas sp. B1 or Pseudomonas fluorescens WW4

Simultaneous Mixture Analysis Using a Dynamic Microbial Sensor Combined with Chemometrics

“…The immobilization procedure was based on previously described methods. − A 250 mg sample of the prepolymer (33.4%) was mixed with 250 μL of 50 mM potassium phosphate buffer (pH 7.2). This hydrosol was cross-linked and neutralized up to a pH between 6.4 and 6.5 by stepwise addition of 10 μL amounts of poly(ethylene imine) (2.5% (w/v) in water) under constant mixing.…”

Methods of Encapsulation of Biomacromolecules and Living Cells. Prospects of Using Metal–Organic Frameworks

“…Polyacrylamide gel is used quite often [103] despite its toxicity. Other carriers such as PVA-based cryogels [104,105], ENT/ENTP photocrosslinked polymers (polymer mixture based on polyethylene or polypropylene glycol, hydroxyethyl acrylate, and isophorone diisocyanate, which is polymerized under near UV irradiation) or modified PVA [106], granules and films based on polyvinyl chloride [107], hydrogels based on chitosan [108][109][110], polycarbamoyl sulfonate [111,112], and polyurethane [113,114], peptide polymers [115], biotin-avidin linkers [116][117][118], solgel matrices based on aluminum oxide [119] or composite polymers [120][121][122][123], as well as electropolymeriz able polyaniline films [124][125][126][127] and other compounds [128,129], and nanostructured materials (including carbon nanotubes and metal nanoparticles) [130][131][132][133][134][135][136][137] should also be noted. A common disadvantage of encapsulation in gels and polymers is diffusion restrictions imposed by the carrier nature, which in some cases appreciably reduce the rate of biocatalysis and the activity of biocomponents.…”

Biosensors for endocrine disruptors

“…For those reasons amperometric biosensors for naphthalene were developed using either Sphingomonas sp. B1 or Pseudomonas fl uorescens WW4 cells immobilized within a polyurethane-based hydrogel [156]. Another biosensor application for the detection of naphthalene and phenanthrene use DNA as a biorecognition element [157].…”