Microbial influences on urinary polyamine excretion

Satink, Hpwm; Hessels, Jan; Kingma, Aw; Vandenberg, Ga; Muskiet, Frits A. J.; Halie,

doi:10.1016/0009-8981(89)90093-4

Cited by 22 publications

(13 citation statements)

References 11 publications

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“…3, IIIB) showed that abolition of DFMO-induced growth inhibition is related to the presence of bacteria in the intestine. As recently shown by us (Satink et al, 1989) in humans, Cad produced by intestinal bacteria is absorbed and excreted in urine. It is therefore conceivable that Pu, especially that produced by intestinal bacteria to counteract a drop in environmental PH due to fermentation of residual carbohydrates, is also able to enter the body and to subsequently abolish DFMO-induced growth inhibition.…”

Section: Irgfuence Of Dietary Residual Carbohydratesmentioning

confidence: 78%

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Microbial flora in the gastrointestinal tract abolishes cytostatic effects of α‐difluoromethylornithine in vivo

Hessels

Kingma

Ferwerda

et al. 1989

Intl Journal of Cancer

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Although treatment with the ornithine decarboxylase inhibitor alpha-difluoromethylornithine (DFMO) leads to depletion of intracellular polyamines and to related growth inhibition in vitro, its cytostatic effects in vivo are disappointing. This may be due to abolition of DFMO-induced growth inhibition by polyamines released during normal body cell turnover, to dietary polyamines, or to putrescine synthesized by the microbial flora in the GI tract. We studied selectively (aerobic) and totally (aerobic + anaerobic) GI tract-decontaminated LI210-bearing mice fed with 3 types of diet differing in their polyamine and carbohydrate residue contents and treated with combinations of intraperitoneal DFMO and oral deuterium-labelled putrescine. Our data show that, irrespective of diet type, total decontamination markedly potentiates the moderate tumor growth inhibition that is caused by DFMO alone. During total decontamination, growth-inhibited L1210 cells accumulate in the G0/G1 phase of the cell cycle. Although orally administered deuterium-labelled putrescine gave rise to deuterium labelling of L1210 putrescine, spermidine and spermine, the polyamine levels in our diets played only a minor role.

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Section: Irgfuence Of Dietary Residual Carbohydratesmentioning

confidence: 78%

“…We have investigated the effect of GI tract decontamination of healthy persons on the excretion of polyamines in their urine. The results showed a dramatic decrease in total Cad, indicating that under normal conditions it is not only produced by the microbial flora, but that it is subsequently absorbed and transported through the body (Satink et al, 1989).…”

mentioning

confidence: 98%

Microbial flora in the gastrointestinal tract abolishes cytostatic effects of α‐difluoromethylornithine in vivo

Hessels

Kingma

Ferwerda

et al. 1989

Intl Journal of Cancer

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“…Polyamines are usually found in miniscule concentrations in extracellular fluid, however the intestinal epithelia are bathed in an environment with up to millimolar concentrations (Osborne and Seidel, 1990;Pegg and McCann, 1982). The rapid turnover of intestinal epithelium mandates an extrinsic source of polyamines, in the colon these are largely provided from the microbiota in the gut lumen (Bardocz et al, 1998;Hessels et al, 1989;Loser et al, 1999;Satink et al, 1989). It is not surprising then that polyamines are present in high concentrations in human milk (Dorhout et al, 1996;Loser, 2000), and contribute to maturation of the gut in the neonatal period (Capano et al, 1994;Dufour et al, 1988;ter Steege et al, 1997;Wild et al, 1993).…”

Section: Introductionmentioning

confidence: 98%

The effects of polyamines on human colonic mucosal function

Rogers

McDermott

Mohan

et al. 2015

European Journal of Pharmacology

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“…Polyamines derived from food are absorbed in the small intestine and thus are unable to fulfil the high metabolic demand for polyamines of the mucosal tissue in the large bowel (Bardocz et al, 1993). Bacteria present in the human gut serve as a possible source of polyamines (Satink et al, 1989). Members of the genera Bacteroides and Fusobacterium and anaerobic cocci are able to synthesize large amounts of putrescine and spermidine in rats (Noack et al, 1998).…”

mentioning

confidence: 99%

Bacteroides intestinalis sp. nov., isolated from human faeces

Bakir

Kitahara

Sakamoto

et al. 2006

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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During studies of the microbiota of human faeces, five strains of Gram-negative anaerobic rods were isolated following growth in a polyamine-deficient medium. These strains belonged to the genus Bacteroides on the basis of 16S rRNA gene sequence data. 16S rRNA gene sequence similarity between one of the strains, 341 T , and recognized species within the genus Bacteroides was <95 %. The DNA G+C content (44 mol%) and major fatty acid composition (anteiso-C 15 : 0 , 32?0 %) supported the affiliation of strain 341T to the genus Bacteroides.Biochemical tests and DNA-DNA hybridization analysis demonstrated that strain 341 T was distinct from Bacteroides uniformis and Bacteroides helcogenes, to which it was related most closely. On the basis of these data, a novel Bacteroides species, Bacteroides intestinalis sp. nov., is proposed with strain 341 T (=JCM 13265 T =DSM 17393 T ) as the type strain.Polyamines such as putrescine, spermidine and spermine are organic cations required for animal cell growth and differentiation and in various steps of DNA, RNA and protein synthesis. Both prokaryotic and eukaryotic cells synthesize polyamines (Capano et al., 1998; McCormack et al., 1998). The polyamines found in the intestinal lumen originate from endogenous and exogenous sources. Polyamines derived from food are absorbed in the small intestine and thus are unable to fulfil the high metabolic demand for polyamines of the mucosal tissue in the large bowel (Bardocz et al., 1993). Bacteria present in the human gut serve as a possible source of polyamines (Satink et al., 1989). Members of the genera Bacteroides and Fusobacterium and anaerobic cocci are able to synthesize large amounts of putrescine and spermidine in rats (Noack et al., 1998). The use of polyamine-deficient media facilitates the isolation of polyamine-producing bacteria (Noack et al., 2000). During an investigation of micro-organisms grown on polyaminedeficient medium, we isolated the strains reported here.Five bacterial strains (strains 276, 281, 341 T , 342 and 344) were isolated from the faeces of healthy Japanese adults (strains 276 and 281 were from a 40-year-old male and the others from a 32-year-old female). The polyamine-deficient medium of Noack et al. (1998), with minor modification, and a standard dilution plate method were used for isolation. The composition of the medium was as follows (mmol l 21 unless indicated): MOPS (40), Tricine (4), K 2 HPO 4 (1?3), NaHCO 3 (119), NaCl (85?5), NH 4 Cl (9?5), glucose (27?3), L-amino acid mixture [alanine (3?6), arginine (1?6), asparagine (1?9), aspartic acid (1?5), cystine (0?12), cysteine (3?3), glutamic acid (2?7), glutamine (3?4), glycine (8?5), histidine (0?6), isoleucine (1?7), leucine (2?8), lysine (12?3), methionine (0?7), ornithine (2?0), phenylalanine (1?3), proline (4?9), serine (2?3), threonine (1?7), tryptophan (0?2), tyrosine (0?7) and valine (2?4) on Eggerth Gagnon (EG) agar (Merck) supplemented with 5 % horse blood for 2 days at 37 uC in an anaerobic jar (Hirayama) filled with 100 % CO 2 .Amplificat...

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Microbial influences on urinary polyamine excretion

Cited by 22 publications

References 11 publications

Microbial flora in the gastrointestinal tract abolishes cytostatic effects of α‐difluoromethylornithine in vivo

Microbial flora in the gastrointestinal tract abolishes cytostatic effects of α‐difluoromethylornithine in vivo

The effects of polyamines on human colonic mucosal function

Bacteroides intestinalis sp. nov., isolated from human faeces

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