Bacteroides intestinalis sp. nov., isolated from human faeces

Bakir, Mohammad Abdul; Kitahara, Maki; Sakamoto, Mitsuo; Matsumoto, Mitsuharu; Benno, Yoshimi

doi:10.1099/ijs.0.63914-0

Cited by 62 publications

(41 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, although cholesterol-reducing activity has never been reported in the genus Bacteroides, steroid metabolism, including bile salt hydrolase, sulfatase, and glucuronidase activities, has been detected in different Bacteroides species (25). Sequences from novel phylotypes of yet-to-be-cultured Bacteroides species have been detected in 16S rRNA gene clone libraries from human fecal samples (11,37), and previously unknown Bacteroides species of human origin have recently been isolated and characterized (1,2,19). A comparison of 16S rRNA gene sequences revealed that the cholesterol-reducing strain D8 clustered with the two isolates described as B. dorei, displaying Ͼ99.5% sequence similarity.…”

Section: Discussionmentioning

confidence: 99%

Bacteroides sp. Strain D8, the First Cholesterol-Reducing Bacterium Isolated from Human Feces

Gérard

Lepercq

Leclerc

et al. 2007

Appl Environ Microbiol

112

View full text Add to dashboard Cite

The microbial community in the human colon contains bacteria that reduce cholesterol to coprostanol, but the species responsible for this conversion are still unknown. We describe here the first isolation and characterization of a cholesterol-reducing bacterium of human intestinal origin. Strain D8 was isolated from a 10 ؊8 dilution of a fresh stool sample provided by a senior male volunteer with a high capacity to reduce luminal cholesterol to coprostanol. Cholesterol-to-coprostanol conversion by strain D8 started on the third day, while cells were in stationary phase, and was almost complete after 7 days. Intermediate products (4-cholesten-3-one and coprostanone) were occasionally observed, suggesting an indirect pathway for cholesterol-to-coprostanol conversion. Resting-cell assays showed that strain D8 could reduce 1.5 mol of cholesterol/mg bacterial protein/h. Strain D8 was a gram-negative, non-spore-forming, rod-shaped organism identified as a member of the genus Bacteroides closely related to Bacteroides vulgatus, based on its morphological and biochemical characteristics. The 16S rRNA gene sequence of strain D8 was most similar (>99.5%) to those of two isolates of the recently described species Bacteroides dorei. Phylogenetic tree construction confirmed that Bacteroides sp. strain D8 clustered within an independent clade together with these B. dorei strains. Nevertheless, no cholesterol-reducing activity could be detected in cultures of the B. dorei type strain. Based on Bacteroides group-specific PCR-temporal temperature gradient gel electrophoresis, there was no correlation between the presence of a band comigrating with the band of Bacteroides sp. strain D8 and cholesterol conversion in 11 human fecal samples, indicating that this strain is unlikely to be mainly responsible for cholesterol conversion in the human population.

show abstract

Section: Discussionmentioning

confidence: 99%

Bacteroides sp. Strain D8, the First Cholesterol-Reducing Bacterium Isolated from Human Feces

Gérard

Lepercq

Leclerc

et al. 2007

Appl Environ Microbiol

112

View full text Add to dashboard Cite

show abstract

“…Members of the genera Bacteroides and Parabacteroides in the order Bacteroidales are known to make up 14 to 40% of cultivable microorganisms in human feces (21 (3,12,23,26,38) and constitute up to 80% of the fecal bacterial isolates from the genus Bacteroides documented in Ribosomal Database Project II (http://rdp.cme.msu.edu).…”

Section: Approximately 10mentioning

confidence: 99%

Relative Abundance ofBacteroidesspp. in Stools and Wastewaters as Determined by Hierarchical Oligonucleotide Primer Extension

Hong

Liu

2008

Appl Environ Microbiol

View full text Add to dashboard Cite

A molecular method, termed hierarchical oligonucleotide primer extension (HOPE), was used to determine the relative abundances of predominant Bacteroides spp. present in fecal microbiota and wastewaters. For this analysis, genomic DNA in feces of healthy human adults, bovines, and swine and in wastewaters was extracted and total bacterial 16S rRNA genes were PCR amplified and used as the DNA templates for HOPE. Nineteen oligonucleotide primers were designed to detect 14 Bacteroides spp. at different hierarchical levels (domain, order, cluster, and species) and were arranged into and used in six multiplex HOPE reaction mixtures. Results showed that species like B. vulgatus, B. thetaiotaomicron, B. caccae, B. uniformis, B. fragilis, B. eggerthii, and B. massiliensis could be individually detected in human feces at abundances corresponding to as little as 0.1% of PCR-amplified 16S rRNA genes. Minor species like B. pyogenes, B. salyersiae, and B. nordii were detected only collectively using a primer that targeted the B. fragilis subgroup (corresponding to ϳ0.2% of PCR-amplified 16S rRNA genes). Furthermore, Bac303-related targets (i.e., most Bacteroidales) were observed to account for 28 to 44% of PCR-amplified 16S rRNA genes from human fecal microbiota, and their abundances were higher than those detected in the bovine and swine fecal microbiota and in wastewaters by factors of five and two, respectively. These results were comparable to those obtained by quantitative PCR and to those reported previously from studies using whole-cell fluorescence hybridization and 16S rRNA clone library methods, supporting the conclusion that HOPE can be a sensitive, specific, and rapid method to determine the relative abundances of Bacteroides spp. predominant in fecal samples.

show abstract

“…For both strains, the most closely related type strain was Bacteroides coprosuis CCUG 50528 T (Whitehead et al, 2005), with a sequence similarity of 95.9 % based on the sequence of strain SV434 T (the aforementioned Y was replaced with T for the similarity calculations). The next most closely related type strains were those of Bacteroides intestinalis (Bakir et al, 2006) (sequence similarity, 93.5 %) and Bacteroides thetaiotaomicron (93.2 %) (Holdeman et al, 1984). Strains SV434 T and S562 formed a branch that was separate from those of closely related Bacteroides species in the phylogenetic tree composed of related Bacteroides species (Fig.…”

mentioning

confidence: 99%

“…(Bakir et al, 2006); 4, B. thetaiotaomicron ATCC 29148 T (Holdeman et al, 1984). +, Positive; 2, negative; ND, no data available.…”

mentioning

confidence: 99%

Bacteroides propionicifaciens sp. nov., isolated from rice-straw residue in a methanogenic reactor treating waste from cattle farms

Ueki

Abe

Kaku

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

View full text Add to dashboard Cite

Two strictly anaerobic bacterial strains (SV434 T and S562) were isolated from rice-straw residue in a methanogenic reactor treating waste from cattle farms in Japan. They had identical 16S rRNA gene sequences and showed almost the same phenotypic properties. The cells of both strains were Gram-negative, non-motile, non-spore-forming rods; extraordinarily long rods often occurred. Remarkable stimulation of growth occurred with the addition of haemin and cobalamin (vitamin B 12 ) to the medium. The supplementary cobalamin and haemin could be replaced if autoclaved and clarified sludge fluid obtained from the reactor was added. Both strains utilized a range of growth substrates, including arabinose, fructose, galactose, glucose, mannose, cellobiose, maltose, glycogen, starch, dextrin, amygdalin, lactate and pyruvate. Both strains produced acetate and propionate with a small amount of succinate from these substrates in the presence of haemin and cobalamin. Both strains were slightly alkaliphilic, having a pH optimum at about 7.9. The temperature range for growth was 5-35 6C, the optimum being 30 6C. The NaCl concentration range for growth was 0-4 % (w/v). Catalase activity was not detected in cells cultivated without haemin, whereas cells cultivated with haemin usually had the enzyme activity. Oxidase and nitrate-reducing activities were not detected. Aesculin was hydrolysed, but gelatin was not hydrolysed. Both strains were sensitive to bile acids. The major cellular fatty acids of both strains were anteiso-C 15 : 0 and iso-C 15 : 0 . Menaquinones MK-8(H 0 ) and MK-9(H 0 ) were the major respiratory quinones and the genomic DNA G+C contents were 46.2-47.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences placed both strains in the phylum Bacteroidetes. Bacteroides coprosuis (isolated from swine-manure storage pits) was the species most closely related to both strains (95.9 % 16S rRNA gene sequence similarity to the type strain). On the basis of the phylogenetic, physiological and chemotaxonomic analyses, strains SV434 T and S562 represent a novel species of the genus Bacteroides, for which the name Bacteroides propionicifaciens sp. nov. is proposed. The type strain is SV434 T (5JCM 14649 T 5DSM 19291 T ).

show abstract

Bacteroides intestinalis sp. nov., isolated from human faeces

Cited by 62 publications

References 20 publications

Bacteroides sp. Strain D8, the First Cholesterol-Reducing Bacterium Isolated from Human Feces

Bacteroides sp. Strain D8, the First Cholesterol-Reducing Bacterium Isolated from Human Feces

Relative Abundance ofBacteroidesspp. in Stools and Wastewaters as Determined by Hierarchical Oligonucleotide Primer Extension

Bacteroides propionicifaciens sp. nov., isolated from rice-straw residue in a methanogenic reactor treating waste from cattle farms

Contact Info

Product

Resources

About