Microbial assimilation of methanol induction and function of catalase in <i>Candida boidinii</i>

Roggenkamp, Rainer; Sahm, Hermann; Wagner, Fritz

doi:10.1016/0014-5793(74)81230-5

Cited by 134 publications

(55 citation statements)

References 13 publications

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“…The reaction was started by the addition of 10 l of lysate from 30 ϫ 10 6 cells, and absorbance at 240 nm was measured for 2 min. The rate of decrease, reflecting catalase activity, was converted to activity by the extinction coefficient 4.36 ϫ 10 4 cm 2 / mol (31).…”

Section: Methodsmentioning

confidence: 99%

Frataxin and Mitochondrial Carrier Proteins, Mrs3p and Mrs4p, Cooperate in Providing Iron for Heme Synthesis

Zhang

Lyver

Knight

et al. 2005

Journal of Biological Chemistry

106

107

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Frataxin is a conserved mitochondrial protein implicated in cellular iron metabolism. Deletion of the yeast frataxin homolog (YFH1) was combined with deletions of MRS3 and MRS4, mitochondrial carrier proteins implicated in iron homeostasis. As previously reported, the ⌬yfh1 mutant accumulated iron in mitochondria, whereas the triple mutant (⌬⌬⌬) did not. When wildtype, ⌬mrs3/4, ⌬yfh1, and ⌬⌬⌬ strains were incubated anaerobically, all strains were devoid of heme and protected from iron and oxygen toxicity. The cultures were then shifted to air for a short time (4 -5 h) or a longer time (15 h), and the evolving mutant phenotypes were analyzed (heme-dependent growth, total heme, cytochromes, heme proteins, and iron levels). A picture emerges from these data of defective heme formation in the mutants, with a markedly more severe defect in the ⌬⌬⌬ than in the individual ⌬mrs3/4 or ⌬yfh1 mutants (a "synthetic" defect in the genetic sense). The defect(s) in heme formation could be traced to lack of iron. Using a real time assay of heme biosynthesis, porphyrin precursor and iron were presented to permeabilized cells, and the appearance and disappearance of fluorescent porphyrins were followed. The Mrs3/4p carriers were required for rapid iron transport into mitochondria for heme synthesis, whereas there was also evidence for an alternative slower system. A different role for Yfh1p was observed under conditions of low mitochondrial iron and aerobic growth (revealed in the ⌬⌬⌬), acting to protect bioavailable iron within mitochondria and to facilitate its use for heme synthesis.

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Section: Methodsmentioning

confidence: 99%

Frataxin and Mitochondrial Carrier Proteins, Mrs3p and Mrs4p, Cooperate in Providing Iron for Heme Synthesis

Zhang

Lyver

Knight

et al. 2005

Journal of Biological Chemistry

106

107

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“…Catalase activity was measured spectrophotometrically at 240 nm by H202 degradation (Roggenkamp et al 1974), MOX activity by oxidation of ABTS with HzO 2 and peroxidase at 420 nm (Eggeling and Sahm 1978), and malate dehydrogenase by oxidation of NADH with oxaloacetate as a substrate (Bergmeyer and Bernt 1983). Measurement of fi-lactamase activity was performed by monitoring degradation of the chromogenic cephalosporin Nitrocefin at 395 nm and cytochrome c oxidase by oxidation of reduced cytochrome c monitored at 550 nm (Tolbert 1974).…”

Section: Methodsmentioning

confidence: 99%

Targeting sequences of the two major peroxisomal proteins in the methylotrophic yeast Hansenula polymorpha

et al. 1992

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“…In these cases, cell-free extracts were prepared by X-pressing and centrifugation (Emri et al, 1997). Specific catalase (Roggenkamp et al, 1974) and glutathione peroxidase (GPx; Chiu et al, 1976) activities were measured. Briefly, catalase and GPx activities were determined spectrophotometrically, measuring H 2 O 2 decomposition and NADPH diminution rates, respectively (Roggenkamp et al, 1974;Chiu et al, 1976).…”

mentioning

confidence: 99%

“…Specific catalase (Roggenkamp et al, 1974) and glutathione peroxidase (GPx; Chiu et al, 1976) activities were measured. Briefly, catalase and GPx activities were determined spectrophotometrically, measuring H 2 O 2 decomposition and NADPH diminution rates, respectively (Roggenkamp et al, 1974;Chiu et al, 1976). In the GPx assay, cumene hydroperoxide was used as substrate and the glutathione disulphide formed was reduced by glutathione reductase auxiliary enzyme, which oxidizes NADPH cofactor (Chiu et al, 1976).…”

mentioning

confidence: 99%

bZIP transcription factors affecting secondary metabolism, sexual development and stress responses in Aspergillus nidulans

et al. 2013

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The eukaryotic basic leucine zipper (bZIP) transcription factors play critical roles in the organismal response to the environment. Recently, a novel YAP-like bZIP, restorer of secondary metabolism A (RsmA), was found in a suppressor screen of an Aspergillus nidulans secondary metabolism (SM) mutant in which overexpression of rsmA was found to partially remediate loss of SM in Velvet Complex mutants. The Velvet Complex is a conserved fungal transcriptional heteromer that couples SM with sexual development in fungi. Here we characterized and contrasted SM in mutants of RsmA and four other A. nidulans bZIP proteins (NapA, ZipA, ZipB and ZipC) with predicted DNA binding motifs similar to RsmA. Only two overexpression mutants exhibited both SM and sexual abnormalities that were noteworthy: OE : : rsmA resulted in a 100-fold increase in sterigmatocystin and a near loss of meiotic spore production. OE : : napA displayed decreased production of sterigmatocystin, emericellin, asperthecin, shamixanthone and epishamixanthone, coupled with a shift from sexual to asexual development. Quantification of bZIP homodimer and heterodimer formation using fluorescence resonance energy transfer (FRET) suggested that these proteins preferentially self-associate.

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Microbial assimilation of methanol induction and function of catalase in Candida boidinii

Cited by 134 publications

References 13 publications

Frataxin and Mitochondrial Carrier Proteins, Mrs3p and Mrs4p, Cooperate in Providing Iron for Heme Synthesis

Frataxin and Mitochondrial Carrier Proteins, Mrs3p and Mrs4p, Cooperate in Providing Iron for Heme Synthesis

Targeting sequences of the two major peroxisomal proteins in the methylotrophic yeast Hansenula polymorpha

bZIP transcription factors affecting secondary metabolism, sexual development and stress responses in Aspergillus nidulans

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