Targeting sequences of the two major peroxisomal proteins in the methylotrophic yeast Hansenula polymorpha

The methylotrophic yeast Hansenula polymorpha: a versatile cell factory

“…The successful use of a PTS1 to accumulate large amounts of a heterologous protein in H. polymorpha peroxisomes has been demonstrated [30,38]. The PTS1 (SKL-COOH) was added to the carboxyterminus of a fusion protein, which consisted of human insulin-like growth factor II and a carrier protein.…”

The N‐terminus of amine oxidase of Hansenula polymorpha contains a peroxisomal targeting signal

“…In apparent contradiction to its small size, a high level of degeneracy of the PTS 1 was shown by site-directed mutagenesis experiments, and variants of this sequence are present at the C-terminus of many peroxisomal proteins [2,3]. Also in H. polymorpha, import of the majority of the peroxisomal matrix proteins is mediated by a PTS1 signal, for example ARF in alcohol oxidase, NKL in dihydroxyacetone synthase and SKI in catalase [4,5].…”

Hansenula polymorpha: An attractive model organism for molecular studies of peroxisome biogenesis and function

“…This is indicated by the finding that not all peroxisomal proteins contain an SKL or SKL-like motif which is particularly true for yeast enzymes [19,20]. Indeed, other sorting signals have been identified [21]. These signals also are predominantly located at the extreme C-terminus although exceptions have been encountered [22].…”

“…Evidently, MOM19 can substitute for MOM72, although at low efficiency. By analogy, import of AO and DHAS in H. polymorpha, which both contain a C-terminal PTS [21], may be facilitated by a general, constitutive import mechanism, suggested before [16] which may partly substitute for a specific, substrate inducible, receptor for amine oxidase. A most intriguing question, however, remains whether different receptors interact with a common protein translocation machinery.…”