Microarray expression profiling of dysregulated long non‐coding <scp>RNA</scp>s in Hirschsprung's disease reveals their potential role in molecular diagnosis

Zheng, Shen; Du, Chong; Zang, Rujin; Xie, Hui; Lv, Wei; Li, H.; Xia, Yankai; Tang, Weibing

doi:10.1111/nmo.12722

Cited by 20 publications

(19 citation statements)

References 31 publications

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“…Recently, evidence have indicated that non-protein coding genes such as miR-218-1 [3], miR-206 [4], miR-192/215 [5] and some other microRNAs (miRNAs), can also result in the occurrence of HSCR by retarding the proliferation and migration of cells. At the same time, some long noncoding RNAs (lncRNAs) are also detected significantly differentially expressed in intestinal tissues with HSCR according to the Microarray analysis [6] which suggests another pathogenic factor for HSCR.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA ZNF609 functions as a competitive endogenous RNA to regulate AKT3 expression by sponging miR-150-5p in Hirschsprung's disease

et al. 2016

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Research over the past decade suggested critical roles for circular RNAs in the natural growth and disease progression. However, it remains poorly defined whether the circular RNAs participate in Hirschsprung disease (HSCR). Here, we reported that the cir-ZNF609 was down-regulated in HSCR compared with normal bowel tissues. Furthermore, suppression of cir-ZNF609 inhibited the proliferation and migration of cells. We screened out several putative cir-ZNF609 ceRNAs of which the AKT3 transcript was selected. Finally, RNA immunoprecipitation and luciferase reporter assays demonstrated that cir-ZNF609 may act as a sponge for miR-150-5p to modulate the expression of AKT3. In conclusion, these findings illustrated that cir-ZNF609 took part in the onset of HSCR through the crosstalk with AKT3 by competing for shared miR-150-5p.

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Section: Introductionmentioning

confidence: 99%

Circular RNA ZNF609 functions as a competitive endogenous RNA to regulate AKT3 expression by sponging miR-150-5p in Hirschsprung's disease

et al. 2016

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“…Thus, we randomly selected an additional 11 lncRNAs for qRT‐PCR verification for a total of 22 lncRNAs, including 10 upregulated and 12 downregulated lncRNAs. An additional 25 samples were selected during the verification process, therefore, the verification results not only tested the consistency between the qRT‐PCR results and microarray data as previously reported, but also verified the expression levels of these selected lncRNAs in ESCC. The qRT‐PCR verification results showed good consistency with the microarray data, and were similar to previously reported data …”

Section: Discussionmentioning

confidence: 66%

Genome‐wide analysis of long non‐coding RNAs in esophageal squamous cell carcinoma reveals their potential role in invasion and metastasis

Wang

Sun

et al. 2018

Thoracic Cancer

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BackgroundA high lymphatic metastasis rate and strong local invasive ability are the key characteristics of esophageal squamous cell carcinoma (ESCC) that affect patient survival, and long non‐coding RNAs (lncRNAs) may play a crucial role. We performed genome‐wide analysis of lncRNAs to identify novel biomarkers associated with local invasion and lymphatic metastasis in ESCC.MethodsSix pairs of ESCC tumor and para‐tumor tissues were subjected to microarray analysis to identify differentially expressed lncRNAs, and 25 pairs of tissues samples were used to verify the effectiveness of screened lncRNAs using quantitative reverse transcription PCR. The correlations between verified lncRNAs and clinicopathological characteristics were analyzed to confirm specific lncRNAs associated with the local invasion and lymphatic metastasis of ESCC, and gene co‐expression analysis was used to predict potential mechanisms.ResultsMicroarray analysis identified 1850 lncRNAs with significant differential expression in ESCC. Of 22 lncRNAs selected for quantitative reverse transcription PCR verification, four were significantly upregulated and one was significantly downregulated in ESCC cancer compared to para‐cancer tissues. ENST00000508406.1 was significantly associated with T, N, and tumor node metastasis stages, and NR_037652.1 was significantly associated with N stage. Moreover, 49 lncRNA‐messenger RNA pairs were significantly associated with the two dysregulated lncRNAs and possibly involved in the regulation of local invasion and lymphatic metastasis of ESCC.ConclusionThe present genome‐wide analysis identified two novel and tumor‐specific lncRNAs for predicting ESCC local invasion and lymphatic metastasis, providing insight into the potential underlying mechanism, which warrants further investigation.

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“…Our prevenient work has proved the expression profile of long non-coding RNA in HSCR [14]. One of them is LOC100507600, which is obviously down regulated among HSCR cases.…”

Section: Introductionmentioning

confidence: 81%

“…Recently long non-coding RNA have been proved to play important roles in a lot of diseases [18][19][20]. According to the Microarray expression results of lncRNAs between HSCR and negative controls [14]. LOC100507600 is significantly down-regulated in HSCR tissues.…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA LOC100507600 functions as a competitive endogenous RNA to regulate BMI1 expression by sponging miR128-1-3p in Hirschsprung's disease

Wen

Shen

et al. 2018

Cell Cycle

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Recently studies reported that long non-coding RNAs (lncRNAs) may take part in a lot of congenital diseases, meanwhile, Hirschsprung's disease (HSCR) is a major congenital digestive tract malformation. Nevertheless whether lncRNAs participate in the occurrence of HSCR and how it contributes to this disease are still unknown. LOC100507600 was selected from our gene expression microarray data obtained from bowel tissues from HSCR patients and negative controls. Subsequently, we used qRT-PCR to prove the result in 64 pairs of HSCR disease bowel stenosis tissues and negative controls. Transwell assay, CCK-8 assay and flow cytometry were employed to explore whether cellular functions change after knocking down the LOC100507600 in SH-SY5Y cell and human 293T cell. Dual-luciferase reporter assay was used to confirm the competitive relationship between BMI1 and LOC100507600 through their association with hsa-miR128-1-3p. Protein extraction and Western blotting were used to further confirm the relationship between LOC100507600 and BMI1. We found that LOC100507600 was obvious reduced in tissues from HSCR patients with noteworthy correlation with BMI1. Furthermore, Downregulation of LOC100507600 repressed cell migration and proliferation and didn't affect cell apoptosis or cycle. Dual-luciferase reporter assay, qRT-PCR and Western blotting assay verified that LOC100507600 serves as a competitive endogenous RNA of miR128-1-3p and down-regulates BMI1 expression by sponging miR128-1-3p in HSCR. In sum, our study researches the potential diagnostic value of LOC100507600 in HSCR and deduces that LOC100507600 can contributes to HSCR as a competitive endogenous RNA to regulate BMI1 expression by sponging miR128-1-3p.

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Microarray expression profiling of dysregulated long non‐coding RNAs in Hirschsprung's disease reveals their potential role in molecular diagnosis

Abstract: Our results are the first to report the expression profile of dysregulated lncRNAs in HSCR and infer that lncRNAs may serve as novel diagnostic biomarkers for HSCR.

Cited by 20 publications

References 31 publications

Circular RNA ZNF609 functions as a competitive endogenous RNA to regulate AKT3 expression by sponging miR-150-5p in Hirschsprung's disease

Circular RNA ZNF609 functions as a competitive endogenous RNA to regulate AKT3 expression by sponging miR-150-5p in Hirschsprung's disease

Genome‐wide analysis of long non‐coding RNAs in esophageal squamous cell carcinoma reveals their potential role in invasion and metastasis

Long non-coding RNA LOC100507600 functions as a competitive endogenous RNA to regulate BMI1 expression by sponging miR128-1-3p in Hirschsprung's disease

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