Long non-coding RNA LOC100507600 functions as a competitive endogenous RNA to regulate BMI1 expression by sponging miR128-1-3p in Hirschsprung's disease

Abstract

Recently studies reported that long non-coding RNAs (lncRNAs) may take part in a lot of congenital diseases, meanwhile, Hirschsprung's disease (HSCR) is a major congenital digestive tract malformation. Nevertheless whether lncRNAs participate in the occurrence of HSCR and how it contributes to this disease are still unknown. LOC100507600 was selected from our gene expression microarray data obtained from bowel tissues from HSCR patients and negative controls. Subsequently, we used qRT-PCR to prove the result in 64 pairs of HSCR disease bowel stenosis tissues and negative controls. Transwell assay, CCK-8 assay and flow cytometry were employed to explore whether cellular functions change after knocking down the LOC100507600 in SH-SY5Y cell and human 293T cell. Dual-luciferase reporter assay was used to confirm the competitive relationship between BMI1 and LOC100507600 through their association with hsa-miR128-1-3p. Protein extraction and Western blotting were used to further confirm the relationship between LOC100507600 and BMI1. We found that LOC100507600 was obvious reduced in tissues from HSCR patients with noteworthy correlation with BMI1. Furthermore, Downregulation of LOC100507600 repressed cell migration and proliferation and didn't affect cell apoptosis or cycle. Dual-luciferase reporter assay, qRT-PCR and Western blotting assay verified that LOC100507600 serves as a competitive endogenous RNA of miR128-1-3p and down-regulates BMI1 expression by sponging miR128-1-3p in HSCR. In sum, our study researches the potential diagnostic value of LOC100507600 in HSCR and deduces that LOC100507600 can contributes to HSCR as a competitive endogenous RNA to regulate BMI1 expression by sponging miR128-1-3p.

RETRACTED ARTICLE: Long noncoding RNA H19 regulates HIF-1α/AXL signaling through inhibiting miR-20b-5p in endometrial cancer

“…In the cytoplasm, lncRNAs can bind to target mRNAs to affect their translation or mediate their stability [23]. In addition, lncRNAs may act in the cytoplasm as competing endogenous RNAs (ceRNAs) [24,25]. In this study, H19 was mainly distributed in the cytoplasm of endometrial cancer, and we speculate that it functions as a ceRNA.…”

Down-regulation of circ-PRKCI inhibits cell migration and proliferation in Hirschsprung disease by suppressing the expression of miR-1324 target PLCB1

“…It has been confirmed that some protein coding genes participate in the pathogenesis of HSCR, including RET, EDNRB, PHOX2B, SOX10, etc [3,4]. To date, some noncoding RNAs have also been proven to take part in the biological processes of HSCR, such as microRNA-939, lncRNA HOTTIP and LOC100507600 [5][6][7]. However, the underlying pathogenesis of HSCR occurrence still remains unclear.…”

Identification of New Potential LncRNA Biomarkers in Hirschsprung Disease

“…Much evidence suggests a relationship between lncRNAs and HSCR, all based on the comparative expression assays of specific lncRNAs in bowel tissue from HSCR patients and controls [73][74][75][76][77][78]. In our study we have performed a differential qRT-PCR assay in the EPCs from HSCR patients and controls, which has allowed us to identify new lncRNAs as potential regulatory elements implicated in ENS development and HSCR onset.…”