Circular RNA ZNF609 functions as a competitive endogenous RNA to regulate AKT3 expression by sponging miR-150-5p in Hirschsprung's disease

Peng, Lei; Chen, Guanglin; Zhu, Zezhang; Zheng, Shen; Du, Chong; Zang, Rujin; Su, Yang; Xie, Hua; Li, Hongxing; Xu, Xiaoqun; Xia, Yankai; Tang, Weibing

doi:10.18632/oncotarget.13656

Cited by 158 publications

(116 citation statements)

References 36 publications

Supporting

Mentioning

112

Contrasting

Unclassified

Order By: Relevance

“…Although the circRNAs have been widely studied in human (Chen et al, 2016; Peng et al, 2016), systematic studies of plant circRNAs are just beginning. The development and senescence of leaves are very complex processes, which involve thousands of genes and multiple layers of regulation.…”

Section: Discussionmentioning

confidence: 99%

“…Some previous studies revealed that circRNAs can regulate gene expression at the transcriptional and posttranscriptional levels (Ashwal-Fluss et al, 2014; Li et al, 2015; Wang, 2015). Furthermore, circRNAs could serve as miRNA sponges by sequestering and preventing microRNAs from binding corresponding target genes (Hansen et al, 2013; Liu Q. et al, 2016; Peng et al, 2016). Nevertheless, the biogenesis and function of circRNAs remains to be studied (Chen and Shan, 2015).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identifying and Characterizing the Circular RNAs during the Lifespan of Arabidopsis Leaves

et al. 2017

View full text Add to dashboard Cite

Leaf growth and senescence are controlled by tight genetic factors involved regulation at multiple levels. Circular RNAs (circRNAs) have recently been reported as the microRNA sponge to accomplish corresponding regulatory roles. This study aims to explore the expression profile and functional role of circRNAs in Arabidopsis leaf growth and senescence. We used publically available RNA-seq data of Arabidopsis leaves to identify the circular RNA expression profile and used quantitative real-time PCR to validate our identified circRNAs. The functions of circRNAs were explored using distinct bioinformatics methods including analysis of network, gene ontology and KEGG pathway. We identified 168 circRNAs, including 40 novel circRNAs, in Arabidopsis thaliana leaves, with 158 (94.1%) circRNAs arising from the exons of genes. Real-time PCRs were used to verify 4 highly expressed circRNAs and they all showed consistent expression patterns with the RNA-seq results. Interestingly, 6 and 35 circRNAs were differentially expressed at G- to -M stage and M- to -S stage, respectively. The circRNAs display an upregulation trend during the lifespan of Arabidopsis leaves. Moreover, the expression of circRNAs during senescence is independent of host gene expression to a certain degree. The gene ontology (GO) and KEGG pathway analysis of the targeted mRNA of circRNA–miRNA–mRNA network showed that the circRNAs may be involved in plant hormone signal transduction, Porphyrin and chlorophyll metabolism during leaves senescence. Our comprehensive analysis of the expression profile of circRNAs and their potential functions during leaf growth and senescence suggest that circRNAs may function as new post-transcriptional regulators in the senescence of Arabidopsis leaves.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identifying and Characterizing the Circular RNAs during the Lifespan of Arabidopsis Leaves

et al. 2017

View full text Add to dashboard Cite

show abstract

“…circRNA-ZNF609 functions as a ceRNA to regulate AKT3 expression by sponging miR-150-5p in Hirschsprung's disease. 8 As a new form of post-transcriptional control, this network has a great potential implication in disease research. In addition, growing evidence suggests that circRNAs are highly enriched in mammalian brain tissues and are often derived from the genes specific for neuronal function.…”

Section: Introductionmentioning

confidence: 99%

Characterization of circRNA-Associated-ceRNA Networks in a Senescence-Accelerated Mouse Prone 8 Brain

et al. 2017

View full text Add to dashboard Cite

Alzheimer's disease (AD) is one of the most common neurodegenerative diseases. Although many researchers have attempted to explain the origins of AD, developing an effective strategy in AD clinical therapy is difficult. Recent studies have revealed a potential link between AD and circRNA-associated-ceRNA networks. However, few genome-wide studies have identified the potential circRNA-associated-ceRNA pairs involved in AD. In this study, we systematically explored the circRNA-associated-ceRNA mechanism in a 7-month-old senescence-accelerated mouse prone 8 (SAMP8) model brain through deep RNA sequencing. We obtained 235 significantly dysregulated circRNA transcripts, 30 significantly dysregulated miRNAs, and 1,202 significantly dysregulated mRNAs. We then constructed the most comprehensive circRNA-associated-ceRNA networks in SAMP8 brain. GO analysis revealed that these networks were involved in regulating the development of AD from various angles, for instance, axon terminus (GO: 0043679) and synapse (GO: 0045202). Following rigorous selection, we discovered that the circRNA-associated-ceRNA networks in this AD mouse model were mainly involved in the regulation of Aβ clearance (Hmgb2) and myelin function (Dio2). This research is the first to provide a systematic dissection of circRNA-associated-ceRNA profiling in SAMP8 mouse brain. The selected circRNA-associated-ceRNA networks can profoundly affect the diagnosis and therapy of AD in the future.

show abstract

“…Indeed, circ‐ZNF609 is necessary for skeletal myoblast proliferation and can be translated into a protein in a splicing‐dependent and cap‐independent manner, providing an example of a protein‐coding circRNA in eukaryotes . circZNF609 is also expressed in neurons as well as in bowel tissues, where it is down‐regulated in Hirschsprung disease …”

Section: Circrna In Cardiovascular Diseasesmentioning

confidence: 99%

Circular RNAs: Methodological challenges and perspectives in cardiovascular diseases

Carrara

Fuschi

Ivan

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

Circular RNAs are generated by back‐splicing of precursor‐mRNAs. Although they have been known for many years, only recently we have started to appreciate their widespread expression and their regulatory functions in a variety of biological processes. Not surprisingly, circular RNA dysregulation and participation in the pathogenic mechanisms have started to emerge in many instances, including cardiovascular diseases. Detection, differential expression analysis and validation are the three critical points for the characterization of any RNA, and circular RNAs are no exception. Their characteristics, however, generate several problems that are yet to be completely addressed, and literature still lacks comprehensive definitions of well‐defined best practices. We present a map of the current knowledge regarding circular RNAs and the critical issues limiting our understanding of their regulation and function. The goal was to provide the readers with the tools to critically decide which of the many approaches available is most suitable to their experimental plan. Although particularly focused on cardiovascular diseases, most critical issues concerning circular RNAs are common to many other fields of investigation.

show abstract

Circular RNA ZNF609 functions as a competitive endogenous RNA to regulate AKT3 expression by sponging miR-150-5p in Hirschsprung's disease

Cited by 158 publications

References 36 publications

Identifying and Characterizing the Circular RNAs during the Lifespan of Arabidopsis Leaves

Identifying and Characterizing the Circular RNAs during the Lifespan of Arabidopsis Leaves

Characterization of circRNA-Associated-ceRNA Networks in a Senescence-Accelerated Mouse Prone 8 Brain

Circular RNAs: Methodological challenges and perspectives in cardiovascular diseases

Contact Info

Product

Resources

About