Microarray Expression Profiles of lncRNAs and mRNAs in Postoperative Cognitive Dysfunction

Abstract

Postoperative cognitive dysfunction (POCD) is serious disorder in the central nervous system common in aged patients after anesthesia. Although its clinical symptoms are well recognized, however, the molecular etiology of the POCD remains unrevealed. Similarly, neither gold standard molecular diagnosis nor effective treatment is available for POCD until the present. Therefore, we aimed to explore the molecular mechanism of this disorder through investigating lncRNAs and mRNAs associated with POCD human patients and investigate their underlying regulatory pathways. In this study, we recruited 200 patients requiring hip or knee replacement surgery. Their neurological functions were assessed at two time points, 1 day before the surgery and 30 days post-surgery. In parallel, serum samples were collected from the participants to analyze lncRNAs and mRNAs differential expression profile between POCD and non-POCD patients using microarray analysis. To further investigate the role differentially expressed mRNA and lncRNAs, Gene Ontology (GO), pathway analyses on mRNAs and lncRNA-mRNA interaction network were performed. As a result, 68 lncRNAs and 115 mRNAs were dysregulated in the POCD group compared to non-POCD group. Among them, the top 10 upregulated lncRNAs and 10 downregulated lncRNAs were listed for enrichment analysis. Interestingly, we found that these lncRNA and mRNA are involved in biological process, molecular function, and cellular component in addition to various signaling pathways, suggesting that the pathogenesis of POCD involves lncRNAs and mRNAs differential expression. Consequently, the genetic dysregulation between the non-POCD and POCD patients participates in the occurrence and development of POCD, and could be served as diagnostic biomarkers and drug targets for POCD treatment.

LncRNA NONMMUT055714 acts as the sponge of microRNA-7684-5p to protect against postoperative cognitive dysfunction

“…LncRNAs represent an important area of research investigating the pathogenesis of POCD. Several recent studies have identified lncRNAs which are differentially expressed in POCD [ 22 – 24 ]. Among these, Chen et al demonstrated that knockdown of the lncRNA PCAI decreased cell death and degree of inflammatory response in BV-2 cells treated with lipopolysaccharide in vitro [ 25 ].…”

Integrative Analysis of lncRNA and mRNA and Profiles in Postoperative Delirium Patients

“…Zhang et al found that 68 lncRNAs were dysregulated in the postoperative cognitive dysfunction group compared to non-postoperative cognitive dysfunction group requiring hip or knee replacement surgery. Among them, the DE lncRNAs mainly participated in process of histone deacetylation ( Zhang et al, 2018 ). In our study, we found that the expression of many lncRNAs was abnormal in patients of POD which results are consent with these published studies.…”

Identification of the Potential Key Circular RNAs in Elderly Patients With Postoperative Cognitive Dysfunction

“…Besides that, Chen and colleagues found that miRNA-146a could protect the cognitive decline induced by surgery trauma by suppressing neuroinflammation in mice (Chen L. et al, 2019). Also, emerging studies showed the differentially expressed lncRNAs and mRNAs and their potential roles in POCD disease as analyzed by microarray (Wei C. et al, 2017;Zhang et al, 2018;Li et al, 2019). Thus, it is necessary to investigate whether the circRNAs, a unique class of ncRNAs, participate in the pathogenesis of POCD.…”