Identification of the Potential Key Circular RNAs in Elderly Patients With Postoperative Cognitive Dysfunction

Abstract

Background: Postoperative cognitive dysfunction (POCD) is one of the severe complications after surgery, inducing low life quality and high mortality, especially in elderly patients. However, the underlying molecular mechanism of POCD remains largely unknown, and the ideal biomarker for clinical diagnosis and prognosis is lacking. Circular RNAs (circRNAs), as a unique class of non-coding RNAs, were characterized by its stability and conservativeness, serving as novel biomarkers in various diseases. Nevertheless, the role of circRNAs in the occurrence of POCD remains elusive. Methods: To investigate the differentially expressed circRNAs in the serum of POCD patients and its potential role in the development of POCD, we performed a circRNA microarray to screen the differentially expressed circRNAs in the serum samples from three patients of the POCD group and three paired patients of the non-POCD group. Subsequently, quantitative real-time polymerase chain reaction analysis (qRT-PCR) was utilized to verify the microarray data with the serum samples from 10 paired patients. Cytoscape software was used to construct the circRNA-miRNA-mRNA network for circRNAs with different expression levels as well as the target genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed the biological functions of the differentially expressed circRNAs target genes. Results: In total, we have analyzed 10,198 circRNAs through the microarray. Compared with the non-POCD patient group, there were 210 differentially expressed circRNAs with 133 upregulated and 77 downregulated in the POCD group (≥2-fold differential expression, P ≤ 0.05). The qRT-PCR confirmed 10 circRNAs with different expressed levels, and the results were consistent with the microarray findings. Among them, hsa_circRNA_001145, hsa_circRNA_101138, and hsa_circRNA_061570 had the highest magnitude of change. The GO analysis showed that the differentially expressed

Noncoding RNAs: Novel Insights into Postoperative Neurocognitive Disorders

“…According to previous studies, ,, profiling of circRNAs was conducted via the following details: (1) total RNA extraction, (2) RNA measurement with NanoDrop, (3) RNA library construction, (4) RNA sequencing (RNA-seq) analysis, (5) FAST-QC and circRbase database, (6) bioinformatic analysis (|log 2 fold change| > 2 and P < 0.05, Miranda and RNA hybrid predicted target genes, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated functions of circRNA, Cytoscape software constructed a circRNA-miRNA-mRNA network), and (7) relative expression using qRT-PCR by the formula 2 –ΔΔ Ct .…”

“…The hsa-circRNA-089763 may bind to miR-7111-3p, miR-6769b-3p, and miR-670-3p, all of which may regulate the IGFBP5 gene . In elderly patients with POCD undergoing elective cardiac surgery, hsa_circRNA_001145 potentially functions with miR-1226-5p as a sponge on the ITSN1 gene; hsa_circRNA_101138-hsa-miR-107-NEDD9 was in the network . A list of roles of ncRNA axes in POCD is presented in Table .…”

Identification of Potential Key circRNAs in Aged Mice With Postoperative Delirium

“…In recent years, studies on the regulation of gene transcription in POD have increased. Gao et al (2020) analyzed the differential expression profile of circRNAs in the blood of POD patients and found that circRNAs are involved in the regulation of development processes, cell adhesion and nervous system development and are enriched in the MAPK signaling pathway and RAS signaling pathway. Wu W. et al (2021) analyzed the expression of circRNAs, miRNAs and mRNAs in the prefrontal cortex by NGS sequencing technology and believed that neuroinflammation was the main pathological mechanism of POD.…”

Up-regulation of miR-20a ameliorates sevoflurane anesthesia-induced cognitive impairment in rats by targeting EphA4

“…MicroRNAs (miRNAs) are a class of small noncoding RNAs that can bind to the 3'-untranslated region (3'-UTR) of the transcript of their homologous target genes, thereby regulating their expression [9]. Extensive studies have confirmed a broad association between miRNAs and anesthesia-related cognitive impairment [9][10][11]; miRNAs, such as miR-665, miR-572, and miR-181, have been reported to affect POCD progression, suggesting that these miRNAs could serve as promising therapeutic targets for treating POCD [12].…”