2004
DOI: 10.1111/j.1464-410x.2004.05016.x
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Microarray analysis of changes in renal phenotype in the ethylene glycol rat model of urolithiasis: potential and pitfalls

Abstract: reaction (RT-PCR, 21 probe sets; control, four rats; treated, five rats). Targets were defined as different by the software if the fold change (FC) was ≥ 2, and sorted into functional categories using a data-mining tool. The repeatability of MA was investigated by subjecting the 4-week samples to MA in two independent runs. RESULTSThe results for targets with a FC of ≥ 2 were plotted ( y = 1.01 x -0.75; r 2 0.84). Comparing the results obtained by RT-PCR and MA showed a good qualitative correlation for those t… Show more

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Cited by 23 publications
(25 citation statements)
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“…More recently, microarray analysis had demonstrated changes in the expression of a large number of genes in the stone-forming rat models [22] but in the present study we did not determine GGCX levels in treated and control experimental models. Although experimental animal models of urolithiasis have been used widely to examine the renal expression of several inhibitory proteins present in urine and stone matrix, sometimes the experimental procedures could cause a biphasic response, and some research probably yielded conflicting results attributed to different species of rats and methods of inducing nephrolithiasis [23] .…”
Section: Discussioncontrasting
confidence: 41%
“…More recently, microarray analysis had demonstrated changes in the expression of a large number of genes in the stone-forming rat models [22] but in the present study we did not determine GGCX levels in treated and control experimental models. Although experimental animal models of urolithiasis have been used widely to examine the renal expression of several inhibitory proteins present in urine and stone matrix, sometimes the experimental procedures could cause a biphasic response, and some research probably yielded conflicting results attributed to different species of rats and methods of inducing nephrolithiasis [23] .…”
Section: Discussioncontrasting
confidence: 41%
“…Rats with hyperoxaluria and CaOx nephrolithiasis induced by ethylene glycol also show increased urinary excretion of lipid peroxides and LDH. 16,17 Recently obtained human data are also suggestive of oxidative stress involvement in kidney stone formation. 18,19 Earlier studies had already shown the presence of injury in the kidneys of idiopathic stone formers.…”
Section: Discussionmentioning
confidence: 95%
“…Clinic studies have provided evidence for the development of oxidative stress (OS) and renal injury in kidney stone patients [4,5]. Results of other studies of hyperoxaluria-induced CaOx nephrolithiasis in rats have confirmed the production of reactive oxygen species (ROS) in association with renal injury [2,3,6,7].…”
Section: Introductionmentioning
confidence: 91%