Micro-two-dimensional TLC in search of selected<i>Mentha</i>sp. Extracts for their composition and antioxidative activity

Hawrył, Mirosław; Niemiec, Małgorzata; Waksmundzka‐Hajnos, Monika

doi:10.1556/jpc.26.2013.2.7

Cited by 6 publications

(4 citation statements)

References 9 publications

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“…The separation and determination of compounds in multicomponent mixtures, that is, plant extracts, are usually performed with the use of high performance liquid chromatography, HPLC (e.g., [ 1 , 2 ]), or planar chromatography (e.g., [ 3 – 5 ]). The sample complicity often involves application of two-dimensional system in both techniques.…”

Section: Introductionmentioning

confidence: 99%

“…The comparison of solute and standard retentions (both measured as the partition coefficient, k ) achieved during two individual analyses is the principal to the compound identification in 2D-HPLC. Similarly, in 2D-TLC, the spots of two mixtures (plant extract and standards) are separated with the use of two chromatographic plates [ 3 – 5 ]. Subsequently, the sample components are identified by comparison of their retardation factor ( R F ) values with those of the separated standards.…”

Section: Introductionmentioning

confidence: 99%

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The New TLC Method for Separation and Determination of Multicomponent Mixtures of Plant Extracts

Matysik

Woźniak

Paduch

et al. 2016

Journal of Analytical Methods in Chemistry

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The new mode of two-dimensional gradient thin layer chromatography (MGD-2D TLC) has been presented. Short distance development of sample in the first dimension leads to formation of the preconcentrated narrow zones. They are consecutively separated in the second dimension with the mobile phase gradient in several steps of development until the eluent reaches the further end of the chromatographic plate. The use of the above-mentioned technique allows isolating and then identifying the compounds of various polarity from the multicomponent mixture. The practical application of two-dimensional gradient thin layer chromatography has been performed for isolation of the two plant (Juniperus and Thymus) oils components as the examples of test mixtures. The experiments have been carried out with the use of silica gel plates as well as a normal phase condition. The results of solute separation with isocratic one-dimensional thin layer chromatography system have been compared with those of two-dimensional gradient system. It has been observed that application of the latter mode leads to almost triplicated number of zones in comparison with the former one. It is purposeful to apply the proposed mode to control the purity of the dominant component or components of the mixture.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The New TLC Method for Separation and Determination of Multicomponent Mixtures of Plant Extracts

Matysik

Woźniak

Paduch

et al. 2016

Journal of Analytical Methods in Chemistry

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“…Modern high-performance planar chromatography including micro-TLC is very useful technique for separation biological samples, generally due to the high sensitivity analysis, short analysis time, low consumption of eluent, and a small amount of the sample [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…All solvents (propan-2-ol, ethyl acetate, acetonitrile, n-heptane, and methanol) were analytical grade and purchased from Polish Reagents (POCh, Gliwice, Poland). To receive nonaqueous phases, propan-2-ol or ethyl acetate was mixed with n-heptane [5], and distilled water was mixed with acetonitrile to receive aqueous solvent for 2D-HPTLC. Acetonitrile and distilled water (concentrations: 5%, 10%, 15%, 20%, and 25% (v/v)) were prepared to optimize the separation of test substances in aqueous systems using DIOL-bonded chromatographic plates as stationary phase.…”

Section: Introductionmentioning

confidence: 99%

Micro-2D-TLC separation of phenolics in some species of mint and their fingerprints on diol bonded polar stationary phase

Hawrył

Niemiec

Słomka

et al. 2016

Acta Chromatographica

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Summary. Micro-thin-layer chromatography in two dimensional (2D-mTLC) mode in normal and reversed phase systems by use of diol bonded stationary phase was applied to make fingerprints of 11 species of Mentha genus and two finished pharmaceutical products.Nonaqueous eluents (propan-2-ol or ethyl acetate dissolved in n-heptane) were used in normal phase systems. Mixtures of acetonitrile with water were used in reversed phase chromatographic systems.Optimization of one dimensional systems was performed by determining of R F vs. composition of mobile phases dependencies for standards occurring in various species of Mentha. Most selective eluents were chosen to optimize two-dimensional systems by creating R F in normal-phase (NP) systems vs. R F in reversed-phase (RP) systems correlations.2D-mTLC on diol polar bonded stationary phase were optimized to separate phenolic compounds and make fingerprints of examined plant materials and this method was never applied earlier in the chromatographic analysis.

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Micro Two-Dimensional Thin-Layer Chromatography and Chemometric Analysis of Essential Oils from Selected Mentha Species and Its Application in Herbal Fingerprinting

Hawrył

Świeboda

Hawrył

et al. 2015

Journal of Liquid Chromatography & Related Technologies

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Micro-two-dimensional TLC in search of selectedMenthasp. Extracts for their composition and antioxidative activity

Cited by 6 publications

References 9 publications

The New TLC Method for Separation and Determination of Multicomponent Mixtures of Plant Extracts

The New TLC Method for Separation and Determination of Multicomponent Mixtures of Plant Extracts

Micro-2D-TLC separation of phenolics in some species of mint and their fingerprints on diol bonded polar stationary phase

Micro Two-Dimensional Thin-Layer Chromatography and Chemometric Analysis of Essential Oils from Selected Mentha Species and Its Application in Herbal Fingerprinting

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