The New TLC Method for Separation and Determination of Multicomponent Mixtures of Plant Extracts

Abstract

The new mode of two-dimensional gradient thin layer chromatography (MGD-2D TLC) has been presented. Short distance development of sample in the first dimension leads to formation of the preconcentrated narrow zones. They are consecutively separated in the second dimension with the mobile phase gradient in several steps of development until the eluent reaches the further end of the chromatographic plate. The use of the above-mentioned technique allows isolating and then identifying the compounds of various polarity from the multicomponent mixture. The practical application of two-dimensional gradient thin layer chromatography has been performed for isolation of the two plant (Juniperus and Thymus) oils components as the examples of test mixtures. The experiments have been carried out with the use of silica gel plates as well as a normal phase condition. The results of solute separation with isocratic one-dimensional thin layer chromatography system have been compared with those of two-dimensional gradient system. It has been observed that application of the latter mode leads to almost triplicated number of zones in comparison with the former one. It is purposeful to apply the proposed mode to control the purity of the dominant component or components of the mixture.

Chemical composition, insecticidal and biochemical effects of two plant oils and their major fractions against Aedes aegypti, the common vector of dengue fever

“…Fractions that showed insecticidal activity, were gathered and purified on a Sephadex LH-20 column using 70% methanol. For further purification, the developed fractions were collected and redeveloped on pre-coated TLC plates (silica gel G 60, F 254 , Merck, Suwanee, GA), using a selective eluent system for each fraction ( Pothier et al., 2001 ; Matysik et al., 2016 ), which afforded two main fractions for each EO. Same fractions were pooled and successively washed using CHCl 3 and MeOH for crystallization.…”

Kinetic optimization of angkak – red ginger extraction and its impact on antioxidant activity

“…The stain from both single angkak and combination TLC also have a similar problem with previous red ginger TLC, which can be seen from a stain with rf 0.06 in single angkak extract that cannot be found in combination extract, and this can be caused by another compound in red ginger that have similar polarity (a stain with rf 0.1 in figure 3 and 4) with that compound in angkak (Matysik et al, 2016).…”

“…It can be seen from a stain with rf 0.5 in the single extract that cannot be found in combination extract, and this can be caused by another compound in angkak that have similar polarity (a stain with rf 0.54 in Figures 5 and 6) with that compound in red ginger (Matysik et al, 2016). However, the specific compound in the stain cannot be ascertained because it requires a comparison.…”

Cupressus sempervirens Essential Oil, Nanoemulsion, and Major Terpenes as Sustainable Green Pesticides against the Rice Weevil

“…Trials were conducted in order to determine the best eluent. To accomplish this, several solutions of both n-hexane: ethyl acetate and toluene: ethyl acetate were prepared, which were then tested using Thin-layer Chromatographic plates (TLC), with toluene: ethyl acetate (90:10, then 93:7) being chosen as the best eluent [26]. According to TLC data, developed fractions were divided into 3 main fractions: Main fraction 1 (fractions 8-14, 274.3 mg) was fractionated on a silica gel column, affording 41 mg of α-pinene.…”

“…According to TLC data, developed fractions were divided into 3 main fractions: Main fraction 1 (fractions 8-14, 274.3 mg) was fractionated on a silica gel column, affording 41 mg of α-pinene. The main fraction 2 (fractions [21][22][23][24][25][26][27]193.1 mg) yielded 23 mg of δ-3-carene. By contrast, when fraction 3 (fractions 31-37, 82.5 mg) was developed it provided 9.2 mg of α-cedrol.…”