Micro-CT at the imaging beamline P05 at PETRA III

Wilde, Fabian; Ogurreck, Malte; Greving, Imke; Hammel, Jörg U.; Beckmann, Felix; Hipp, Alexander; Lottermoser, L.; Khokhriakov, Igor; Lytaev, Pavel; Dose, T.; Burmester, Hilmar; Müller, Martin; Schreyer, A.

doi:10.1063/1.4952858

Cited by 138 publications

(83 citation statements)

References 7 publications

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“…Parameters characterizing the degradation of the material were determined and the non‐destructive nature of SRµCT enabled the subsequent analysis of the elemental composition of the material using micro X‐ray fluorescence spectroscopy (µXRF). The presented flow cell is in its second generation and was designed for the micro‐computed tomography beamline P05 at storage ring PETRA III . The first generation flow cell was previously successfully tested at the beamlines BW2 and W2 of the storage ring DORIS III at the Deutsches Elektronen‐Synchrotron DESY …”

Section: Introductionmentioning

confidence: 99%

Quantitative characterization of degradation processes in situ by means of a bioreactor coupled flow chamber under physiological conditions using time‐lapse SRµCT

Zeller‐Plumhoff

Helmholz

Feyerabend

et al. 2017

Materials & Corrosion

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Magnesium and its alloys have in recent years emerged as a promising alternative to titanium‐based implants for medical applications due to favorable degradation properties and good biocompatibility. The degradation of materials is currently investigated by studying different samples of the same material at different time points after degradation in a medium. This study is presenting a high‐resolution time‐lapse investigation of Mg‐2Ag in culture medium using synchrotron radiation‐based micro‐computed tomography over the course of 5 days. The design of the custom‐built corrosion cell and bioreactor are described. The computed degradation rate after 5 days is in agreement with the literature. SRµCT enables the segmentation of cracks forming in the degradation layer due to stresses and hydrogen development.

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Section: Introductionmentioning

confidence: 99%

Quantitative characterization of degradation processes in situ by means of a bioreactor coupled flow chamber under physiological conditions using time‐lapse SRµCT

Zeller‐Plumhoff

Helmholz

Feyerabend

et al. 2017

Materials & Corrosion

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“…All SR‐

μ

CT analyses were conducted at the beamline P05 of the storage ring PETRA III (Deutsches Elektronen‐Synchrotron – DESY, Hamburg, Germany) operated by Helmholtz‐Zentrum Geesthacht (Haibel et al ., ; Greving et al ., ). Although one unstained specimen (in absolute ethanol) was measured using single grating differential phase‐contrast ( = DPC) setup as previously described (Hipp et al ., ; Wilde et al ., ), the remaining specimens were analysed using attenuation contrast (Greving et al ., ), herein referred to as standard SR‐

μ

CT setup. The photon energy applied varied between 8 and 23 keV according to the electron density of samples treated with distinct contrasting methods (Table ).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of contrasting techniques for X‐ray imaging of velvet worms (Onychophora)

Jahn

Oliveira

Gross

et al. 2018

Journal of Microscopy

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Non-invasive imaging techniques like X-ray computed tomography have become very popular in zoology, as they allow for simultaneous imaging of the internal and external morphology of organisms. Nevertheless, the effect of different staining approaches required for this method on samples lacking mineralized tissues, such as soft-bodied invertebrates, remains understudied. Herein, we used synchrotron radiation-based X-ray micro-computed tomography to compare the effects of commonly used contrasting approaches on onychophorans - soft-bodied invertebrates important for studying animal evolution. Representatives of Euperipatoides rowelli were stained with osmium tetroxide (vapour or solution), ruthenium red, phosphotungstic acid, or iodine. Unstained specimens were imaged using both standard attenuation-based and differential phase-contrast setups to simulate analyses with museum material. Our comparative qualitative analyses of several tissue types demonstrate that osmium tetroxide provides the best overall tissue contrast in onychophorans, whereas the remaining staining agents rather favour the visualisation of specific tissues and/or structures. Quantitative analyses using signal-to-noise ratio measurements show that the level of image noise may vary according to the staining agent and scanning medium selected. Furthermore, box-and-whisker plots revealed substantial overlap in grey values among structures in all datasets, suggesting that a combination of semiautomatic and manual segmentation of structures is required for comprehensive 3D reconstructions of Onychophora, irrespective of the approach selected. Our results show that X-ray micro-computed tomography is a promising technique for studying onychophorans and, despite the benefits and disadvantages of different staining agents for specific tissues/structures, this method retrieves informative data that may eventually help address evolutionary questions long associated with Onychophora.

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“…Imaging of the fossil using SRμCT (Fig. ) was done at the beamline P05 of the storage ring PETRA III (Deutsches Elektronen‐Synchrotron—DESY, Hamburg, Germany) operated by Helmholtz‐Zentrum Geesthacht (Haibel et al., ; Greving et al., ; Wilde et al., ). The amber piece was mounted on a beamline standard sample‐stub with beeswax and imaged using an attenuation contrast setup (Greving et al., ).…”

Section: Methodsmentioning

confidence: 99%

Synchrotron X‐ray imaging of a dichasium cupule of Castanopsis from Eocene Baltic amber

Sadowski

Hammel

Denk

2018

American J of Botany

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Premise of the Study The Eocene Baltic amber deposit represents the largest accumulation of fossil resin worldwide, and hundreds of thousands of entrapped arthropods have been recovered. Although Baltic amber preserves delicate plant structures in high fidelity, angiosperms of the “Baltic amber forest” remain poorly studied. We describe a pistillate partial inflorescence of Castanopsis (Fagaceae), expanding the knowledge of Fagaceae diversity from Baltic amber. Methods The amber specimen was investigated using light microscopy and synchrotron‐radiation‐based X‐ray micro‐computed tomography (SRμCT). Key Results The partial inflorescence is a cymule, consisting of an involucre of scales that surround all four pistillate flowers, indicating a dichasium cupule. Subtending bracts are basally covered with peltate trichomes. Flowers possess an urecolate perianth of six nearly free lobes, 12 staminodia hidden by the perianth, and a tri‐locular ovary that is convex‐triangular in cross section. The exceptional three‐dimensional preservation suggests that the fossil belongs to the extant East Asian genus Castanopsis. The amber inclusion represents the first record of Castanopsis from Baltic amber and the first pistillate inflorescence of Fagaceae from Eurasia. Conclusions The partial female inflorescence reported here provides an important addition to acorns of Castanopsis described from middle Eocene strata of Europe. Furthermore, the intercontinental distribution of Castanopsis in the Eocene is confirmed. The amber fossil also broadens the picture of the Baltic amber source area, indicating oligotrophic, sandy, bog‐like habitats. Finally, this study underscores the great benefit of SRμCT as a powerful tool to investigate plant inclusions from amber in a nondestructive way.

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Micro-CT at the imaging beamline P05 at PETRA III

Abstract: In-vacuum multi-modal monochromator for synchrotron-based hard x-ray micro-imaging AIP Conference Proceedings 1741, 040025 (2016)

Cited by 138 publications

References 7 publications

Quantitative characterization of degradation processes in situ by means of a bioreactor coupled flow chamber under physiological conditions using time‐lapse SRµCT

Quantitative characterization of degradation processes in situ by means of a bioreactor coupled flow chamber under physiological conditions using time‐lapse SRµCT

Evaluation of contrasting techniques for X‐ray imaging of velvet worms (Onychophora)

Synchrotron X‐ray imaging of a dichasium cupule of Castanopsis from Eocene Baltic amber

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