Micelle size characterization of lipopeptides produced by B. subtilis and their recovery by the two-step ultrafiltration process

Jauregi, Paula; Coutte, François; Catiau, Lucie; Lecouturier, Didier; Jacques, Philippe

doi:10.1016/j.seppur.2012.11.017

Cited by 55 publications

(51 citation statements)

References 26 publications

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“…Conventional and unconventional methods for the recovery of LP have been reported in recent years. [85][86][87] The main advantage of isolation and purification methods is their ability to operate in a continuous mode for recovering LP with high level of purity. A single downstream processing technique is often not enough for the recovery and purification of LP/BS.…”

Section: Isolation and Purification Of Lpmentioning

confidence: 99%

Screening concepts, characterization and structural analysis of microbial-derived bioactive lipopeptides: a review

Biniarz

Łukaszewicz

Janek

2016

Critical Reviews in Biotechnology

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Lipopeptide biosurfactants are surface active biomolecules that are produced by a variety of microorganisms. Microbial lipopeptides have gained the interest of microbiologists, chemists and biochemists for their high biodiversity as well as efficient action, low toxicity and good biodegradability in comparison to synthetic counterparts. In this report, we review methods for the production, isolation and screening, purification and structural characterization of microbial lipopeptides. Several techniques are currently available for each step, and we describe the most commonly utilized and recently developed techniques in this review. Investigations on lipopeptide biosurfactants in natural products require efficient isolation techniques for the characterization and evaluation of chemical and biological properties. A combination of chromatographic and spectroscopic techniques offer opportunities for a better characterization of lipopeptide structures, which in turn can lead to the application of lipopeptides in food, pharmaceutical, cosmetics, agricultural and bioremediation industries. ARTICLE HISTORY

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Section: Isolation and Purification Of Lpmentioning

confidence: 99%

Screening concepts, characterization and structural analysis of microbial-derived bioactive lipopeptides: a review

Biniarz

Łukaszewicz

Janek

2016

Critical Reviews in Biotechnology

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“…A 50 µL of sample was injected in each run, which lasted for 60 min, and eluent absorbance was monitored at 214 nm. The system was calibrated using standard surfactin (>99.8%) [19][20]. the surfactin concentration was determined by HPLC and the purity was determined in terms of mass of surfactin over the total dry weight mass.…”

Section: Determination Of Surfactin Concentrationmentioning

confidence: 99%

“…The concentration of protein in the solutions of semi-purified biosurfactant was determined by the bicinchoninic acid method [19][20].…”

Section: Protein Concentrationmentioning

confidence: 99%

“…The literature describes the production of surfactin per liter of culture medium (glucose as carbon source and extracted direct from the culture medium) to be at least 500 mg/L, whereas using glycerol as carbon source, lower yields are usually described (158.14 mg/L after 72 h of fermentation) [5], [19][20]. The production with AGG medium was ≈ 325 mg of surfactin/liter of culture medium, whereas with the CGBP medium was 71 mg/L.…”

Section: Measurement Of Surface Activity -Collapsed Foam and Culture mentioning

confidence: 99%

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Comparative study: bench-scale surfactin production from bacillus subtilis using analytical grade and concentrated glycerol from the biodiesel industry

Andrade¹,

Simiqueli

Andrade³

et al. 2016

Int. J. Sci. World

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The market price of glycerol worldwide tends to decrease, since it is a by-product of biodiesel production. Thus its biotechnological use might lead to significant reduction in the cost of fermentations. The aim of this study was to compare the production of surfactin in peptone culture media supplemented with analytical grade glycerol (AGG) and concentrated glycerol from biodiesel production (CGBP). Differences were observed between the two processes including cell growth and dissolved oxygen consumption. Surfactin yield was 325.19 mg/L with AGG and 71.13 mg/L with CGBP, which proves the impact and importance of the purity of glycerol on the yield of surfactin. In addition, five surfactin homologous were identified by ESI-Q-TOFMS, which were composed by two amino acid sequences ELLMDLD and ELLLDLL. Therefore, as surfactin is a high value-added product, the use of glycerol with high purity is fundamental to achieve higher productivity.

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“…In order to obtain the highest solubility, surfactin was solubilized in buffer pH 8.5 at 100 mg.L -1 [9]. The emulsification index was measured using the method described by Cooper and Goldenberg [10], whereby 6 mL of each crude oil (light, medium and heavykindly provided by CEPETRO-UNICAMP, which were collected in Brazil), benzene (Sigma-Aldrich > 99 %), toluene (SigmaAldrich > 99.3 %) and xylenes (Sigma-Aldrich ≥ 98.5 %), were added to 4 mL of biosurfactant solutions: surfactin standard, MEL-B standard; produced surfactin and produced MEL-B.…”

Section: Emulsification Indexmentioning

confidence: 99%

Comparative study on microbial enhanced oil recovery using mannosylerithritol lipids and surfactin

Andrade

Pastore²

2016

Int. J. Sci. World

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Worldwide oil production has been declining. Microbial enhanced oil recovery is one of the most important tertiary recovery processes. The aim of this work was to evaluate the surface activity properties of surfactin and mannosylerithritol lipids-B. In our previous studies, surfactin and mannosylerithritol lipids were produced using cassava wastewater as substrate and then purified by ultrafiltration. Thus, this work extends our previous studies. Experiments of surface activity under extreme conditions (temperature, ionic strength and pH), oil displacement, removal of oil from sand and emulsification index were carried out. Central composite rotational design was performed under extreme conditions of temperature, pH and ionic strength. The results indicated that ionic strength significantly affected the surface activity of surfactin. On the other hand, ionic strength, but also temperature and pH significantly affected the tenso activity of mannosylerithritol lipids-B. Regarding oil displacement test, mannosylerithritol lipids-B showed higher clear zone than surfactin. Contrary, in the experiments of removal of crude oil from sand, minimal differences were observed between surfactin and mannosylerithritol lipids-B. Therefore, both surfactin and mannosylerithritol lipids-B showed good surface activity under extreme conditions. In addition, it seems that mannosylerithritol lipids-B is subtly better than surfactin for microbial enhanced oil recovery.

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Micelle size characterization of lipopeptides produced by B. subtilis and their recovery by the two-step ultrafiltration process

Cited by 55 publications

References 26 publications

Screening concepts, characterization and structural analysis of microbial-derived bioactive lipopeptides: a review

Screening concepts, characterization and structural analysis of microbial-derived bioactive lipopeptides: a review

Comparative study: bench-scale surfactin production from bacillus subtilis using analytical grade and concentrated glycerol from the biodiesel industry

Comparative study on microbial enhanced oil recovery using mannosylerithritol lipids and surfactin

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