Comparative study: bench-scale surfactin production from bacillus subtilis using analytical grade and concentrated glycerol from the biodiesel industry

Abstract

The market price of glycerol worldwide tends to decrease, since it is a by-product of biodiesel production. Thus its biotechnological use might lead to significant reduction in the cost of fermentations. The aim of this study was to compare the production of surfactin in peptone culture media supplemented with analytical grade glycerol (AGG) and concentrated glycerol from biodiesel production (CGBP). Differences were observed between the two processes including cell growth and dissolved oxygen consumption. Surfactin yield was 325.19 mg/L with AGG and 71.13 mg/L with CGBP, which proves the impact and importance of the purity of glycerol on the yield of surfactin. In addition, five surfactin homologous were identified by ESI-Q-TOFMS, which were composed by two amino acid sequences ELLMDLD and ELLLDLL. Therefore, as surfactin is a high value-added product, the use of glycerol with high purity is fundamental to achieve higher productivity.

Dairy and Wine Industry Effluents as Alternative Media for the Production of Bacillus-Based Biocontrol Agents

“…Hence, different alternative media, mostly based on agroindustrial residues, have been suggested as possible routes to decrease the surfactin production cost. Raw glycerol from the biodiesel industry has been suggested as a medium basis for surfactin production, resulting in a surfactin yield in the range of 71–286 mg/L [ 65 , 66 ]. Brewery wastewater, as a surfactin production medium, resulted in a surfactin yield of 6.6 mg/L [ 67 ], while distillers’ grains applied as a substrate gave a better result, with a yield of 1.04 g/L [ 13 ].…”

Production of microbial biosurfactants: Status quo of rhamnolipid and surfactin towards large‐scale production

“…Design of experiments is often employed to investigate the influence of different substances on surfactin productivity [63]. While most published results are based on synthetic media such as mineral salt medium with glucose as carbon source, also cultivations based on alternative substrates such as cashew apple juice, cassava flour, whey or glycerol from biodiesel industry were investigated [64][65][66]. Such research aims at both increasing the surfactin yields, while lowering the overall production costs by using by-products or agricultural/food processing waste streams.…”

Produção de surfactina por >i<Bacillus subtilis>/i< ATCC 21332 utilizando a glicerina residual como substrato e avaliação de seu efeito antimicrobiano contra bactérias redutoras de sulfato

“…A literatura reporta uma grande diversidade de substratos utilizados como fonte de carbono para a produção de surfactina, tais como glicerina residual (ANDRADE et al, 2017;SOUSA et al, 2014;CRUZ et al, 2018); melaço de cana de açúcar (ROCHA et al, 2020); polpa de sisal (ABADIA, 2015); suco de caju (FELIX et al, 2019) e manipueira (NITSCHKE et al, 2004;ANDRADE et al, 2016).…”

“…A Tabela 2 mostra alguns trabalhos recentes em que a glicerina residual é utilizada para a produção de surfactina. De acordo com Andrade et al (2017), a produção média de surfactina em meio mineral utilizando a glicose como substrato é em média 0,5 g/L.…”

“…Andrade et al (2017) investigaram em seu estudo a influência das impurezas da glicerina proveniente da produção de biodiesel na produção de surfactina por Bacillus subtilis LB2b. De acordo com os autores, os cultivos em que foi utilizada a glicerina residual como única fonte de carbono apresentaram crescimento celular significativamente mais baixo em comparação com os cultivos contendo glicerina padrão.…”