Micellar electrokinetic chromatography with laser induced fluorescence detection shows increase of putrescine in erythrocytes of Parkinson's disease patients

Feasibility of Localized Metabolomics in the Study of Pancreatic Islets and Diabetes

“…The current studies further demonstrate that aqueous humor samples can be collected from mice and pooled in sufficient volumes to allow localized non-targeted metabolomic studies. Although not shown here, targeted metabolomics can also be performed on aqueous humor samples from individual mice using other techniques that require nanoliter sample volumes, such as capillary zone electrophoresis and laser-induced fluorescence detection (CZE-LIFD) [36,37]. Importantly, the current studies showed that systemic metabolites relevant to pancreatic islets and diabetes were well represented in the aqueous humor; thus, providing proof-of-concept evidence for using aqueous humor samples, that may be even more enriched with islet- and immune-related metabolites after intraocular islet transplant, in targeted and non-targeted localized metabolomics approaches [23].…”

A novel fluorescent probe of alkyne compounds for putrescine detection based on click reaction

“…25 However, most uorescence sensors need specic conditions such as high temperature or catalyst, which limits the uorescence sensors in the detection of putrescine. 26 Fortunately, Kolb et al discovered a new organic chemistry strategy, click chemistry, which revitalized the old way of organic synthesis. 27 Then Qin et al used CuCl to catalyze the activation of internal alkynes and aromatic amines, and successfully developed a simple and efficient Cu(I)-catalyzed amino-alkyne click polymerization.…”