Visible light-responsive vanadium-based metal–organic framework supported pepsin with high oxidase mimic activity for food spoilage monitoring

Sharifnezhad, Amir Hossein; Dashtian, Kheibar; Zare‐Dorabei, Rouholah; Mahdavi, Mohammad

doi:10.1007/s00604-022-05554-5

Cited by 12 publications

(3 citation statements)

References 71 publications

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“…To investigate enzyme activity and establish the relationship between Fe–CeO 2 /MITiO 2 and enzyme steady-state kinetic parameters, a study of the Michaelis–Menten behaviors was conducted using TMB as the substrate. The Michaelis–Menten equation, V 0 = ( V max [S])/( K m + [S]), was employed to determine the initial velocity ( V 0 ), maximal reaction velocity ( V max ), the concentration of TMB substrate ([S]), and the Michaelis constant ( K m ). ,− By performing a series of nonenzymatic experiments on Fe–CeO 2 /MITiO 2 , the concentration of the TMB substrate was varied while keeping other parameters constant at different time points. The reaction rates for TMB oxidation were calculated, and this data was used to generate standard Michaelis–Menten curves (see Figure g).…”

Section: Resultsmentioning

confidence: 99%

Prussian Blue Analogues-Derived Molecularly Imprinted Nanozyme Array for Septicemia Detection

Dashtian,

Afshar Gheshlaghi,

Zare-Dorabei

et al. 2024

ACS Appl. Bio Mater.

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Septicemia, a severe bacterial infection, poses significant risks to human health. Early detection of septicemia by tracking specific biomarkers is crucial for a timely intervention. Herein, we developed a molecularly imprinted (MI) TiO 2 −Fe−CeO 2 nanozyme array derived from Ce[Fe(CN) 6 ] Prussian blue analogues (PBA), specifically targeting valine, leucine, and isoleucine, as potential indicators of septicemia. The synthesized nanozyme arrays were thoroughly characterized using various analytical techniques, including Fourier transform infrared spectroscopy, X-ray diffraction, fieldemission scanning electron microscope, and energy-dispersive X-ray. The results confirmed their desirable physical and chemical properties, indicating their suitability for the oxidation of 3,3′,5,5′-tetramethylbenzidine serving as a colorimetric probe in the presence of a persulfate oxidizing agent, further highlighting the potential of these arrays for sensitive and accurate detection applications. The MITiO 2 shell selectively captures valine, leucine, and isoleucine, partially blocking the cavities for substrate access and thereby hindering the catalyzed TMB chromogenic reaction. The nanozyme array demonstrated excellent performance with linear detection ranges of 5 μM to 1 mM, 10−450 μM, and 10−450 μM for valine, leucine, and isoleucine, respectively. Notably, the corresponding limit of detection values were 0.69, 1.46, and 2.76 μM, respectively. The colorimetric assay exhibited outstanding selectivity, reproducibility, and performance in the detection of analytes in blood samples, including C-reactive protein at a concentration of 61 mg/L, procalcitonin at 870 ng/dL, and the presence of Pseudomonas aeruginosa bacteria. The utilization of Ce[Fe(CN) 6 ]-derived MITiO 2 − Fe−CeO 2 nanozyme arrays holds considerable potential in the field of septicemia detection. This approach offers a sensitive and specific method for early diagnosis and intervention, thereby contributing to improved patient outcomes.

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Section: Resultsmentioning

confidence: 99%

Prussian Blue Analogues-Derived Molecularly Imprinted Nanozyme Array for Septicemia Detection

Dashtian,

Afshar Gheshlaghi,

Zare-Dorabei

et al. 2024

ACS Appl. Bio Mater.

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“…However, the in‐depth mechanism profile, to date, is still rare. Among various nanozymes, crystalline porous frameworks (such as metal organic frameworks (MOFs)) [21a,24] featuring atom‐precise skeletons and multiple active sites are ideal platforms for revealing the catalytic mechanism. For instance, the peroxidase‐mimicking reactivity of heterojunction‐like bimetallic Ce/Cu quasi‐MOF (Q‐MOF) was enhanced by means of designing photosensitive energy band with high separating efficiency of photoelectron‐hole pairs under visible light, in which the photogenerated h + is located in the VB of CuCeO x cluster while the photogenerated e − flows to the CB of MOF skeleton (Figure 2c).…”

Section: Visible Light‐nanozymementioning

confidence: 99%

Photonanozyme with Light Mediated Activity

Gong,

Tong,

Wang

et al. 2023

ChemPlusChem

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Since the discovery that Fe3O4 nanoparticle has intrinsic natural peroxidase‐like activity by Yan et al in 2007, mimicking native enzymes via nano‐engineering (named as nanozyme) pays a new avenue to bypass the fragility and recyclability of natural enzymes and thus expedites the biocatalysis in multidisciplinary applications. In addition, the high programmability and structural stability attributes of nanozyme afford the ease of coupling with electromagnetic waves of different energies, providing great opportunities to construct photo‐responsive nanozyme under user‐defined electromagnetic waves, which is known as photo‐nanozyme. In this concept, we aim to providing a summary of how electromagnetic waves with varying wavelengths can serve as external stimuli to induce or enhance the biocatalytic performance of photo‐nanozymes, thereby offering fascinating functions that cannot be achieved by pristine nanozyme.

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“…Moreover, metalloporphyrins organic linkers, including M–N x sites, are generated as the two pyrrole protons (N–H) in the rigid macrocycles of porphyrin are substituted by metal ions. Metalloporphyrin‐based nanozymes have proven with various mimic enzyme activities containing peroxidase (POD)‐, oxidase (OXD)‐, and superoxide dismutase (SOD)‐like activity (Amourizi et al., 2020; Sharifnezhad et al., 2022, 2023; Talebi, Dashtian, Zare‐Dorabei, Amourizi, et al., 2023; Talebi, Dashtian, Zare‐Dorabei, Ghafuri, et al., 2023). However, the accessibility to the active sites of MOFs is still challenging.…”

Section: Introductionmentioning

confidence: 99%

Zirconium‐porphyrin‐MOF‐based oxidase‐like nanozyme with oxygen vacancy for aflatoxin B1 colorimetric sensing

Zhang,

Li,

Xia

et al. 2024

Journal of Food Science

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In this study, a porous coordination network zirconium‐porphyrin‐based nanoparticle with oxygen vacancies (OVs) was prepared using acetic acid and benzoic acid as modulators via a simple hydrothermal method. The presence of OVs was confirmed by various characterization methods and was found to enhance oxygen uptake and activation. This resulted in the generation of more reactive peroxyl radicals (•O2−) and led to an improved oxidase (OXD) mimetic activity. Additionally, it promoted 2,2′‐azino‐bis(3‐ethylbenzthiazoline‐6‐sulfonic acid) (ABTS) oxidation, with a low Km value of 0.07 mM and a high Vmax of 1.47 × 10−7 M·s−1. As aflatoxin B1 (AFB1) inhibits the Pt@PCN‐222‐ABTS nanozyme system, a colorimetric probe for AFB1 detection was constructed. The limit of detection (LOD) was 0.074 µg·L−1. This research presents a novel approach for designing a nanozymatic‐based colorimetric method to analyze trace AFB1 residues in food.

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Visible light-responsive vanadium-based metal–organic framework supported pepsin with high oxidase mimic activity for food spoilage monitoring

Cited by 12 publications

References 71 publications

Prussian Blue Analogues-Derived Molecularly Imprinted Nanozyme Array for Septicemia Detection

Prussian Blue Analogues-Derived Molecularly Imprinted Nanozyme Array for Septicemia Detection

Photonanozyme with Light Mediated Activity

Zirconium‐porphyrin‐MOF‐based oxidase‐like nanozyme with oxygen vacancy for aflatoxin B1 colorimetric sensing

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