Abstract:-This study aimed to evaluated different methodologies and water potentials for priming of maize seeds, in order to increase vigor. The experiment was carried out following a completely randomized design, in a factorial scheme 4x3+1, with four water potentials (0.0, -0.4, -0.8 and -1.2 MPa), three priming methods (immersion in non-aerated solution, immersion in aerated solution, paper roll) and a control. After priming, the seeds were dried and evaluated for water content, percentage and first germination coun… Show more
“…The first germination count is a test associated with germination but that expresses seed vigor. Pallaoro et al (2016), when studying the effect of physiological priming on corn seeds, also found higher percentages of normal seedlings in the first count, and different from what was observed in this study, the authors also emphasized improvements in the final seed germination percentage.…”
Section: Resultscontrasting
confidence: 99%
“…Sediyama et al (2012) found a reduction in damage and lesions in soybean seeds after the use of osmopriming. Pallaoro et al (2016) also saw this effect when using an osmotic agent in the priming of corn seeds.…”
The physiological priming has been used to standardize and increase the speed of development of seed lots. The use of this technique is essential in coffee cultivation, as the crop has low longevity associated with slow and uneven germination. The objective was to evaluate the effect of physiological priming on the physiological quality of coffee seeds stored with different water contents. Seeds of two cultivars of Coffea arabica were used: cv. Catuaí Vermelho IAC144 and Topázio MG1190. A portion of the seeds were dried in the shade until reaching 12% moisture, and the other did not undergo drying. The seeds were stored in a cold chamber at 10 °C for nine months. Every three months, the physiological priming technique was performed with subsequent evaluation of the physiological quality by germination tests, root protrusion, normal seedlings at fifteen days, strong normal seedlings, seedlings with expanded cotyledonary leaves and seedling dry weight. Seeds that did not undergo drying subjected to the physiological priming technique maintained physiological quality after nine months of storage. Physiological priming was detrimental to dry seeds stored for nine months.
“…The first germination count is a test associated with germination but that expresses seed vigor. Pallaoro et al (2016), when studying the effect of physiological priming on corn seeds, also found higher percentages of normal seedlings in the first count, and different from what was observed in this study, the authors also emphasized improvements in the final seed germination percentage.…”
Section: Resultscontrasting
confidence: 99%
“…Sediyama et al (2012) found a reduction in damage and lesions in soybean seeds after the use of osmopriming. Pallaoro et al (2016) also saw this effect when using an osmotic agent in the priming of corn seeds.…”
The physiological priming has been used to standardize and increase the speed of development of seed lots. The use of this technique is essential in coffee cultivation, as the crop has low longevity associated with slow and uneven germination. The objective was to evaluate the effect of physiological priming on the physiological quality of coffee seeds stored with different water contents. Seeds of two cultivars of Coffea arabica were used: cv. Catuaí Vermelho IAC144 and Topázio MG1190. A portion of the seeds were dried in the shade until reaching 12% moisture, and the other did not undergo drying. The seeds were stored in a cold chamber at 10 °C for nine months. Every three months, the physiological priming technique was performed with subsequent evaluation of the physiological quality by germination tests, root protrusion, normal seedlings at fifteen days, strong normal seedlings, seedlings with expanded cotyledonary leaves and seedling dry weight. Seeds that did not undergo drying subjected to the physiological priming technique maintained physiological quality after nine months of storage. Physiological priming was detrimental to dry seeds stored for nine months.
“…Immersion methods cause rapid water uptake compared to solid osmotic media that rehydrates seed slowly. Rapid, uncontrolled imbibition is often associated with more electrolyte leakage since desiccation damaged cell membranes need time to reconstitute (Pallaoro et al, 2016) which could explain the slower rate of germination and related parameters.…”
Germination, early emergence and stand establishment of crops are major yield determining factors in semi-arid and arid rainfed areas. Farmers in marginal and low input areas tend to have poor germination due to poor and shallow soils. The objective of this study was to evaluate the effect of priming regimes on seed germination of field crops. A study was conducted at Lupane State University, Biotechnology Laboratory in 2019. Treatments included seed priming techniques, namely hydropriming, halopriming (2% NaCl solution) osmopriming (10% PEG 6000), and solid matrix priming (18% volume/weight sand); and timing at 6, 12, 24 hours for maize and 2, 4, 6 hours for cowpea, sorghum and millet. Solid matrix priming, followed by hydropriming significantly (P<0.05) improved germination parameters (germination percentage, daily germination, peak value and germination speed) of all crops. Halopriming and osmopriming were similar in their ineffectiveness, and resulted in the least germination parameter values. Six hour solid matrix priming in 18% v/w sand was the most effective method among most crops; suggesting that solid matrix priming is an effective and possibly low cost technology, with potential to improve germination of field crops.
Key words: Seed hydration, semi-arid, solid matrix priming
“…Studies on the effect of priming on low temperature tolerance of maize are frequently limited to an individual hybrid genotype within a study to enable comparisons among priming methods. Multiple studies have compared hydropriming with other priming agents with hydropriming consistently showing less improvement in germination or emergence [ 16 – 22 ]. Micronutrient or osmotic priming agents can improve germination and early seedling development at sub-optimal temperatures [ 19 , 20 , 22 , 23 ].…”
Section: Introductionmentioning
confidence: 99%
“…Multiple studies have compared hydropriming with other priming agents with hydropriming consistently showing less improvement in germination or emergence [ 16 – 22 ]. Micronutrient or osmotic priming agents can improve germination and early seedling development at sub-optimal temperatures [ 19 , 20 , 22 , 23 ]. Some hormones, reducing/oxidizing chemicals, chitosan, and even Moringa oliferia leaf extracts have been shown to increase germination or seedling emergence in low temperatures [ 16 – 18 , 20 , 21 , 24 , 25 ].…”
Seeds planted in early spring frequently experience low temperature stress in the soil during germination and early plant growth. Seed pretreatments such as priming have been shown to ameliorate the negative effects of cold soil in some crops. However, the potential beneficial effects of priming have not been widely investigated for Zea mays (maize). To investigate seed priming effects, 24 diverse maize inbred lines were primed using a synthetic solid matrix, Micro-Cel E, and then exposed to 10°C soil conditions. Six DSLR cameras captured time lapsed images of emerging seedlings. Manual scoring was used to determine treatment effects on three seedling emergence metrics. Chilling substantially reduced total emergence for two of 24 genotypes evaluated. For these genotypes, priming provided protection allowing nearly full emergence. Priming significantly reduced mean emergence time and increased the emergence uniformity of chilling sensitive genotypes. The results suggest that the cold sensitive genotypes may benefit from priming pretreatment. Kernel density, weight, oil, protein, and starch traits, as determined by single-kernel near infrared spectroscopy, were not correlated with seedling emergence traits supporting a conclusion that early seedling performance cannot be determined from these maize kernel characteristics.
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