Méthodes photométriques de dosage du cholestérol dans le sérum

Vanzetti, G

doi:10.1016/0009-8981(64)90168-8

Cited by 21 publications

(8 citation statements)

References 69 publications

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Section: Discussionmentioning

confidence: 99%

“…The effect of color reagent composition is best understood with reference to the Liebermann-Burchard and Salkowski reactions (33,50). The characteristic green color of the Liebermann-Burchard reaction is produced when cholesterol reacts with sulfuric acid to form bis(cholestadienyl)monosulfonic acid (33). At high sulfuric acid concentrations the bis(cholestadienyl)monosulfonic acid is further sulfonated to produce the red bis(cholestadienyl)disulfonic acid characteristic of the Salkowski reaction (33).…”

Section: Discussionmentioning

confidence: 99%

“…Adding dehydrating reagent (factor B) to the decanted supernatant and waiting a specified time (factor C) removes water from the sample. Reaction of the cholesterol with color reagent (factor D) produces the green color characteristic of the Liebermann-Burchard reaction (33). Because the colored product, once formed, decays with time, color development time (factor E) is also potentially important.…”

Section: Methodsmentioning

confidence: 99%

“…The manual colorimetric method of Carr and Drekter (28) for determination of total cholesterol in blood serum was chosen as a benchmark against which to test the experimental designs. The method is based on the Liebermann-Burchard reaction (29, 30) which has been noted by numerous authors for its complexity of response (31)(32)(33)(34)(35)(36). The amounts and proportions of reagents and solvents, color development time, amount of water present, temperature, and the presence of interferences are all factors that have been mentioned for their effects on the intensity and stability of the color formed.…”

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confidence: 99%

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Simplex optimization of analytical chemical methods

Morgan¹,

Deming²

1974

Anal. Chem.

333

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Simplex optimization of analytical chemical methods

Morgan¹,

Deming²

1974

Anal. Chem.

333

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“…This report covers a study of the precision and accuracy obtainable with the AutoAnalyzer-N-24 method. Manual ferric chloride methods similar in principle to the AutoAnalyzer-N-24 method have been generally found to give high results when compared to the Abell-Kendall method (7) which is considered a reference method by most authorities (8)(9)(10). In the present investigation, however, the ratio of rates of flow of premixed color reagent to rate of flow of sample extract, and the time of heating of the color reaction, have been adjusted so that the results obtained with the AutoAnalyzer-N-24 method are not high but on the average agree quite well with the values found with the Abell-Kendall procedure.…”

Section: Introductionmentioning

confidence: 99%

Variables in the technicon‐N‐24 method for the determination of serum cholesterol

Roland

Cooper

Witter

1966

J Americ Oil Chem Soc

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A modification of the Teehnicon-N-24 method for serum cholesterol is described. It is possible to obtain precise results which agree on the average with those obtained with the reference Abell-Kendall method. The level of cholesterol found by the AutoAnalyzer method depends primarily on the ratio of the rates of flow of the lipid extract to that of the premixed color reagent and the time of heating of the color reaction at 95C. In this modification, the flow rate of isopropyl alcohol extract of sanlple or standard is kept at 0.9 m!/min and the ratio of flow of extract to that of premixed color reagent is maintained between 1:10 and 1:12 while the time of heating of the color reaction is maintained between 95 and 120 see. Other conditions necessary for precise results are discussed. The precision obtained on the average in several experiments ranged from 2.2 to 2.7 rag% as standard deviation between duplicates.

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Zur Gaschromatographischen Bestimmung Von Cholesterol in Lebensmitteln

Mahlberg

1982

Nahrung

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In der vorliegenden Arbeit soll die Anwendbarket der gaschromatographiechen Bestimmung von Cholesterol in Lebensmitteln (Methode A) im Vergleich zur kolorimetrischen (Methode B) (1) und enzymatischen (Methode C) (2) iberpriiU werden.Durchfiihrung der gaschromatographischen Bestimmung in Anlehnung a n KOVACS, A = ON und ACKMA N (3) 0,5 bis 2,0 g homogenisiertes U&ersuchungsmaterial werden in ein 3O-ml-Zextrifugenglas eirgewogen (die Probe soll ungefghr 0,5 bis 1.5 mg Cholesterol edha2ten). Nach Zugabe von 4 m l ethanolischer Kalilauge (frisch hergestellt aus 25 ml Ethanol, 9@,ig, 5 m l Wasser und 5 g Kaliumhydroxbd) wird die Probe unter gelegentlichem Rtihren 15 rnin in einem Wasserbad auf 66 C erhitzt. Nach Abkiihlen auf Zimmertemperatur und Zugabe von 5 m l Wasser wird 5mal rnit je 5 ml Hexan extrahiert. Bei einer schlecnen Phasentre nnung muR 4 min bei 3000 V/min zentrifilgiert werden. und mit 1 , O ml Hexan aufgenommen. 3 &11 dieser LBsung werden zur gaschromatographischen Analyse unter nachstehenden Bedilgungen verwendet: Chromatron-Gaschromatograph GCHF 18.3 Flammenionisat ionsdet ektor Glastrennskle ( L m e 100 cm, Innendurchmesser 3 mm) T r k e r m a t e r i a l : Chromosorb W-AW-DMCS, 80/100 mesh Trennflitssigkeit: 3 ' % OV-1 Shlentemperatur: 260 "C Injektor-und Detektortemperatur: 290 OC Trt@ergas: 40 m l Stick+aff/miri Eilgangswiderstand: 1 0 &?Empfindlichkeit: 1 Die Retentionszeit fUr Cholesterol b&r#gt 2 min. men, indem Eichlbungen in Konzentrafionen von 150 bis 1500 mg Cholesterol/l Hexan hergestel€t und urter den gleichen Bedingumen wie die Probenlbungen injiziert werden. Die vereinigten Extrakte werden i n einem 25-mlSptzkolben zur Trockne gedampfi Zur quartitafiven Auswertung wird eine Eichung m t 8uLuOerem Standard vorgenom-Ergebnisse und Diskussion Zur Ermittlung der Reproduzierbarket wurden die Standardabweichungen der beschriebenen Mkhoden bei zwei verechiedenen Lebensmftteln bestimmt fTab. 1). Die enzymatische und gaschromatographische Methode aind demnach in der Genauigkeft vergleichbar und weisen eine bessere Prilzision als daf~ klassische kolorimetrische' Verfahren auf (4). Die mft der letzteren Bestimmung gefundenen hBheren Cholesterolgehalte lassen sich durch die geringere S p e z i f t t erkliiren (5). Die gaschromatqraphische Mikrobestimmung von Cholesterol ist im Vergleich zu anderen gebr3uchlichen Met hoden ein vereinfacttes Verfahren. Sie ist empfindlich, zeitsparend und kommt rnit geringen Lkungsmfttel-und Chemikalienmelgen aus. Voraussicitlich ist sie bei einer groBen Anzahl von Lebensmitteln anwendbar. Nach Tab. 2 liegen die Wiederfindungsrafen zwischen 98,l und 100.3 (%. Me gefundenen Cholesterolgehalte einiger Lebensmttel (2.B. mageres Schweinefleisch 38, mageres Rindfleisch 49, Frische RahmbMer, 45 % Fett, 82 mg/lOO g) stimmen

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Méthodes photométriques de dosage du cholestérol dans le sérum

Cited by 21 publications

References 69 publications

Simplex optimization of analytical chemical methods

Simplex optimization of analytical chemical methods

Variables in the technicon‐N‐24 method for the determination of serum cholesterol

Zur Gaschromatographischen Bestimmung Von Cholesterol in Lebensmitteln

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