Variables in the technicon‐N‐24 method for the determination of serum cholesterol

Abstract

A modification of the Teehnicon-N-24 method for serum cholesterol is described. It is possible to obtain precise results which agree on the average with those obtained with the reference Abell-Kendall method. The level of cholesterol found by the AutoAnalyzer method depends primarily on the ratio of the rates of flow of the lipid extract to that of the premixed color reagent and the time of heating of the color reaction at 95C. In this modification, the flow rate of isopropyl alcohol extract of sanlple or standard is kept at 0.9 m!/min and the ratio of flow of extract to that of premixed color reagent is maintained between 1:10 and 1:12 while the time of heating of the color reaction is maintained between 95 and 120 see. Other conditions necessary for precise results are discussed. The precision obtained on the average in several experiments ranged from 2.2 to 2.7 rag% as standard deviation between duplicates.