Method Validation by GC-MS for Analysis of Carvacrol and trans-β-Caryophyllene in Commercial Samples Containing Essential Oils

Abstract

Carvacrol and trans-β-caryophyllene are widely found several medicinal plants, being assigned to these compounds various pharmacological properties. Therefore, this work presents a validation method for these compounds in the species of Lippia sidoides and Rosmarinus officinalis and a copaiba oil sample available in popular trade in Fortaleza. Thus, essential oil samples were diluted and analyzed in a gas chromatograph with mass spectrometric detector (GC-MS). Validation parameters showed satisfactory values, such as linearity, lower limit of detection and quantitation, reproducibility at different days (inter-day) considerable analysis and recovery values. Of the compounds studied, the Lippia sidoides species presented a percentage of 32.01 of carvacrol and 1.78 of trans-β-caryophyllene, respectively. To R. officinales found a percentage of 0.28 and 0.34 respectively for carvacrol and trans-β-caryophyllene. For oil copaiba not logged carvacrol, however, the trans-β-caryophyllene, was present in amount of, 74.5%. With the results, the method can be considered as a useful analytical tool for quality control of trans-β-caryophyllene and carvacrol and the derivatives in the essential oils.

Avaliação dos compostos voláteis majoritários e graxos dos óleos extraídos das farinhas de melancia (Citrullus lanatus Thumb. Manf. var. Crimson Sweet Extra)

“…Inexistem na literatura, trabalhos que citam a presença de compostos voláteis em óleo fixo, carecendo de mais informações. Visto que, vários voláteis, apresentam importantes ações biológicas como antifúngica, antibacteriana, antioxidante, biodegradável, anti-inflamatória e como agentes biológicos no controle de insetos, moluscos, antifúngicos, dentre outros (DOS SANTOS et al, 2018;OLIVEIRA et al, 2017;RIBEIRO-SANTOS et al, 2017).…”