Methicillin-resistant Staphylococcus aureus ST80-IV clone in children from Jordan

Khalil, Wissam; Hashwa, Fuad; Shihabi, Asem; Tokajian, Sima

doi:10.1016/j.diagmicrobio.2012.03.012

Cited by 23 publications

(36 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most commonly occurring spa types were t044 and t037. The high prevalence of spa type t044 was in concordance with several other studies including: Vindel et al [19] who showed that this type was widely disseminated in Europe, Tokajian et al [20] reporting that 38% of S. aureus isolates belonged to spa type t044 in Lebanon, and Khalil et al [11] revealing that 18% of MRSA recovered from stool and nose specimens from children in Jordan were of spa type t044. spa type t044 belongs to the one of the recently emerging PVLpositive MRSA strains (ST80-MRSA-IV), known to occur and predominate in certain regions in Europe and in the Middle East [21].…”

Section: Discussionsupporting

confidence: 78%

“…Genotyping based on spa and MLST revealed fourty-eight different spa types and identified distinct allelic profiles or STs, with the majority belonging to ST80. PFGE of fifteen different spa types revealed eight different pulsotypes, while SCCmec showed the predominance (53%) of subtype IV [11]. Taking into consideration the changing epidemiology of S. aureus, this study was conducted to determine the clinical, epidemiological, and molecular features of currently circulating MSSA and MRSA strains.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular typing of Staphylococcus aureus collected from a Major Hospital in Amman, Jordan

Bazzoun

Harastani

Shehabi

et al. 2014

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Over the past decade methicillin-resistant Staphylococcus aureus (MRSA) has been recognized as a major cause of healthcare associated infections. Recently, however, epidemiology of this pathogen has changed drastically with the emergence of new clones in the community. Efficient epidemiological typing methods are essential to monitor and limit the occurrence and spread of epidemic clones. Methodology: A total of sixty S. aureus isolates were collected from the Jordan University hospital in Amman-Jordan. All isolates were characterized using Staphylococcus protein A (spa) typing and pulsed-field gel electrophoresis (PFGE). Samples were tested for their susceptibility patterns against seven antimicrobial agents and for their potential to form biofilms. Results: spa typing showed that spa type t044 was the most common representing 28% of the isolates studied and 38% of the MRSA population. PFGE revealed fourty-six pulsotypes among the sixty tested isolates clustering similar spa types together. The predominant resistance was detected against levofloxacin, chloramphenicol and clindamycin. One MSSA isolate typed as spa t955 showed biofilm formation potential through protein deposition.. Conclusion: The study results are based on one hospital, but the findings of this and other studies conducted in the region indicate that there is an urgent need for standardized surveillances combined with the application of well-validated typing methods to assess the occurrence of MRSA and to control its spread.

show abstract

Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Molecular typing of Staphylococcus aureus collected from a Major Hospital in Amman, Jordan

Bazzoun

Harastani

Shehabi

et al. 2014

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…6,7 This clone is also known to be frequent in North African and Middle East countries such as Tunisia, Jordan, and Kuwait. 1,17,31 However, the heterogeneity of the MRSA strains isolated in this study was to some extent a surprising finding compared to the large predominance of ST80-IV in the preceding investigations. Noteworthy, the two tst1 positive ST22-MRSA-IV strains, spa type t223, appeared to be similar to the ''Gaza strain,'' the predominant MRSA clone in healthy children and their parents living in the Gaza strip.…”

Section: Discussionmentioning

confidence: 43%

“…Noteworthy, the two tst1 positive ST22-MRSA-IV strains, spa type t223, appeared to be similar to the ''Gaza strain,'' the predominant MRSA clone in healthy children and their parents living in the Gaza strip. 2 This CC22-MRSA-IV ''Middle Eastern Variant'' has been also described in Jordan and Saudi Arabia, 17,26 and more recently, in newborns and preschool children in Palermo, Italy. 10,11 The detection of this clone in Algeria confirms its wide dissemination in the Mediterranean and Middle East area.…”

Section: Discussionmentioning

confidence: 94%

Descriptive Epidemiology of Nasal Carriage ofStaphylococcus aureusand Methicillin-ResistantStaphylococcus aureusAmong Patients Admitted to Two Healthcare Facilities in Algeria

Djoudi

Benallaoua

Aléo

et al. 2015

Microbial Drug Resistance

View full text Add to dashboard Cite

show abstract

“…Moreover, a previous study conducted in the same geographic region, demonstrated that methicilli-nresistant S. aureus (MRSA) strains colonizing infants carried 1-3 clinically important staphylococcal toxin genes [20]. In Lebanon, a recent study on antimicrobial susceptibility patterns of S. aureus clinical isolates revealed that only 56% were susceptible to all tested antibiotics [21]. In addition, the prevalence of MRSA infection are also increased in Lebanon in the last decade [22,23].These reports have led to renewed interest in the usage of MLS to treat staphylococcal infections [24].…”

Section: Discussionmentioning

confidence: 99%

Characterization of resistance genes to macrolides, lincosamides and streptogramins (MLS) among clinical isolates of Staphylococcus aureus in North Lebanon

Osman

Nasbeh²,

Rafei³

et al. 2015

int. arab. j antimicrob. agents

View full text Add to dashboard Cite

Objective: Staphylococcus aureus is one of the most significant pathogens causing significant morbidity and mortality. Moreover, the incidence of MLS S. aureus (resistant at least for one macrolide) infections continues to grow globally. The aim of this study is to examine the expression of resistance of S. aureus isolates to MLS and the prevalence of genes involved in this resistance using PCR. Methods:A total 38 strains of S. aureus MLS-resistant were isolated in the Microbiology Labs at Nini Hospital in North Lebanon. The disk diffusion method was used to determine the phenotype of the MLS resistance. The resistance genes involved were detected by PCR using specific gene primers for ermA, ermB, ermC, msrA, linA, mefA, vat and vgb genes.Results: A total of 55.3% of the isolates were positive for inducible phenotype (iMLS B ), of these 15.8% were positive for the constitutive phenotype (cMLS B ), 23.7% for MS B phenotype and 5.2% for L phenotype. The ermC gene was the most prevalent (52.6%), while ermA, ermB, msrA and linA genes were observed with lower prevalence. A combination of several of these genes was detected,whereas the vgb, vat and mefA genes were not detected in any of the clinical isolates. Conclusion:This study is the first investigated characterization of MLS resistance genes in clinical isolates of S. aureus in Lebanon according to

show abstract

Methicillin-resistant Staphylococcus aureus ST80-IV clone in children from Jordan

Cited by 23 publications

References 19 publications

Molecular typing of Staphylococcus aureus collected from a Major Hospital in Amman, Jordan

Molecular typing of Staphylococcus aureus collected from a Major Hospital in Amman, Jordan

Descriptive Epidemiology of Nasal Carriage ofStaphylococcus aureusand Methicillin-ResistantStaphylococcus aureusAmong Patients Admitted to Two Healthcare Facilities in Algeria

Characterization of resistance genes to macrolides, lincosamides and streptogramins (MLS) among clinical isolates of Staphylococcus aureus in North Lebanon

Contact Info

Product

Resources

About