Methicillin-resistant Staphylococcus argenteus misidentified as methicillin-resistant Staphylococcus aureus emerging in western Sweden

Hallbäck, Erika Tång; Karami, Nahid; Adlerberth, Ingegerd; Cardew, Sofia; Ohlén, Maria; Jakobsson, Hedvig E.; Stadler, Liselott Svensson

doi:10.1099/jmm.0.000760

Cited by 29 publications

(20 citation statements)

References 20 publications

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“…In Myanmar, prevalence of S. argenteus was reported as 0.9% (5/563) from healthy nasal carriers [14] and 2.9% (4/137) [5] in clinical isolates. In contrast, the prevalence seems to be low in European countries (< 1%, Belgium (0.16%) [19], Sweden (0.3%) [21] and Denmark (0.35%) [3], and eastern China (0.7%) [4]. In our present study, the prevalence (ratio of S. argenteus to S. aureus ) in the northern Japan was revealed to be 0.55% (0.85% to MSSA), that was lower than Southeast Asian countries but comparable to those of European countries and eastern China.…”

Section: Discussionmentioning

confidence: 99%

“…Staphylococcus argenteus is a novel species of coagulase-positive staphylococci previously described as a divergent lineage of S. aureus [1], and has been increasingly reported worldwide as an emerging pathogen affecting both humans [2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26] and animals [1,25,27,28,29]. The major characteristic of this lineage is non-pigmented (white) colonies on blood agar plates due to lack of the crtOPQMN gene operon required for staphyloxanthin pigment production [1,2].…”

Section: Introductionmentioning

confidence: 99%

“…The type strain of S. argenteus MSHR1132 T belongs to ST1850 grouped into clonal complex 75 (CC75) [1]. After the first report of genetically divergent ST75 in Australia in 2002 [6,7], S. argenteus belonging to CC75 and other CC represented by CC1223, CC2198, CC2250, and CC2596 were increasingly reported in other Oceanian countries (New Zealand [8] and Fiji [8,9]), Asia (Thailand [10,11], Lao PDR [12], Cambodia [13], Myanmar [5,14], China [4], Japan [15,16,17] and Taiwan [18]), Europe (Belgium [19], France [20], the UK [10], Denmark [3] and Sweden [21]) and South America (French Guiana [22] and Trinidad and Tobago [23]). There are at least three geographical ‘hot spots’ of S. argenteus , such as Southeast Asia, remote human populations in Australia and the Amazon [24,25].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Epidemiological Characterization of Staphylococcus argenteus Clinical Isolates in Japan: Identification of Three Clones (ST1223, ST2198, and ST2550) and a Novel Staphylocoagulase Genotype XV

et al. 2019

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Staphylococcus argenteus, a novel emerging species within Staphylococcus aureus complex (SAC), has been increasingly reported worldwide. In this study, prevalence of S. argenteus among human clinical isolates, and their clonal diversity and genetic characteristics of virulence factors were investigated in Hokkaido, the northern main island of Japan. During a four-month period starting from March 2019, twenty-four S. argenteus and 4330 S. aureus isolates were recovered from clinical specimens (the ratio of S. argenteus to S. aureus :0.0055). Half of S. argenteus isolates (n = 12) belonged to MLST sequence type (ST) 2250 and its single-locus variant, with staphylocoagulase genotype (coa-) XId, while the remaining isolates were assigned to ST2198/coa-XIV (n = 6), and ST1223 with a novel coa-XV identified in this study (n = 6). All the isolates were mecA-negative, and susceptible to all the antimicrobials tested, except for an ST2198 isolate with blaZ and an ST2250 isolate with tet(L) showing resistance to ampicillin and tetracyclines, respectively. Common virulence factors in the S. argenteus isolates were staphylococcal enterotoxin (-like) genes sey, selz, sel26, and sel27 in ST2250, selx in ST2198, and enterotoxin gene cluster (egc-1: seg-sei-sem-sen-seo) in ST1223 isolates, in addition to hemolysin genes (hla, hlb, and hld) distributed universally. Elastin binding protein gene (ebpS) and MSCRAMM family adhesin SdrE gene (sdrE) detected in all the isolates showed high sequence identity among them (> 97%), while relatively lower identity to those of S. aureus (78–92%). Phylogenetically, ebpS, sdrE, selx, sey, selw, sel26, and sel27 of S. argenteus formed clusters distinct from those of S. aureus, unlike sec, selz, tst-1, and staphylokinase gene (sak). The present study revealed the prevalence of S. argenteus among clinical isolates, and presence of three distinct S. argenteus clones (ST2250; ST2198 and ST1223) harboring different virulence factors in northern Japan. ST2198 S. argenteus, a minor clone (strain BN75-like) that had been rarely reported, was first identified in Japan as human isolates.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Epidemiological Characterization of Staphylococcus argenteus Clinical Isolates in Japan: Identification of Three Clones (ST1223, ST2198, and ST2550) and a Novel Staphylocoagulase Genotype XV

et al. 2019

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“…This includes strategies such as specific antibodies or small chemical compounds to target not only Hla directly but also regulatory factors of the agr, sae, and sarA systems and the receptor ADAM10 [30], with the hope that this type of anti-virulence therapies might serve as a complement to antimicrobials. Given the high expression levels of Hla in S. argenteus and that many S. argenteus isolates are shown to be methicillinresistant [8,18,31]these approaches might also be important to combat S. argenteus infections.…”

Section: Discussionmentioning

confidence: 99%

Staphylococcus argenteus and Staphylococcus schweitzeri are cytotoxic to human cells in vitro due to high expression of alpha-hemolysin Hla

2019

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Staphylococcus argenteus and Staphylococcus schweitzeri are newly identified species of the S. aureus -related complex. S. argenteus, as occurring globally and showing significant prevalence and comparable infection and morbidity rates compared to S. aureus , is becoming clinically important. Whole genome sequencing has revealed the presence of several virulence genes but the molecular mechanisms of S. argenteus infection and virulence are largely unknown. Here, we studied the effect of a previously characterized clinical S. argenteus isolate on human cells in vitro . The clinical isolate, together with the S. argenteus type strain MSHR1132T and the S. schweitzeri type strain FSA084T, had a cytotoxic effect on the cells, which showed necrotic cell death after a few hours of treatment. The protein causing the cytotoxic effect was purified and identified by mass spectrometry as alpha-hemolysin, Hla, which is awell-known pore-forming toxin in S.aureus . The cytotoxic effect could be blocked with an antibody against Hla. S.argenteus showed 12–15 fold higher expression levels of hla at the RNA level and 4–6 fold higher expression levels at the protein level compared to S.aureus . The higher expression levels of hla were supported by higher RNA levels of the regulatory factors sarA and saeR . Also, the RNAIII component of the accessory gene regulator (agr) quorum sensing system was 8,000–10,000 fold higher in the S.argenteus isolates compared to S.aureus . This is the first study on the effect of S.argenteus on ahuman cell line and strengthens the idea of significant virulence of S.argenteus .

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“…From Europe the reports are sparse; 25 S. argenteus genomes from Denmark [8], low prevalence (0.16%) in a nationwide Belgian study [9], a case report from France [10] and one strain from UK [3]. However, among isolates classically identified as methicillin-resistant S. aureus (MRSA) in Sweden that is normally considered a low prevalence country regarding MRSA, S. argenteus has also been identified [11,12].…”

Section: Staphylococcus Aureusmentioning

confidence: 99%

Staphylococcus argenteus as an etiological agent of prosthetic hip joint infection: a case presentation

Söderquist

Wildeman

Stenmark

et al. 2020

J. Bone Joint Infect.

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Abstract. This report presents a case of prosthetic hip infection caused by Staphylococcus argenteus, a potentially overlooked etiology of prosthetic joint infections (PJIs). Whole-genome sequencing showed that the S. argenteus isolate was an ST2250 and clustered within other CC2250 isolates, the largest clonal group of S. argenteus. This sequence type is prevalent and may be associated with invasive infections. The present isolate was phenotypically fully susceptible to all tested antimicrobial agents and genome analysis did not detect any resistance genes, nor were any staphylococcal cassette chromosome residues detected. Despite initial appropriate management with debridement and biofilm-active antibiotics, the outcome was unfavorable with recurrence and a persistent infection treated with suppressive antibiotics. Regarding the repertoire of genomic traits for virulence in S. argenteus, PJIs caused by this bacterium should be treated accordingly as Staphylococcus aureus PJIs.

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Methicillin-resistant Staphylococcus argenteus misidentified as methicillin-resistant Staphylococcus aureus emerging in western Sweden

Cited by 29 publications

References 20 publications

Molecular Epidemiological Characterization of Staphylococcus argenteus Clinical Isolates in Japan: Identification of Three Clones (ST1223, ST2198, and ST2550) and a Novel Staphylocoagulase Genotype XV

Molecular Epidemiological Characterization of Staphylococcus argenteus Clinical Isolates in Japan: Identification of Three Clones (ST1223, ST2198, and ST2550) and a Novel Staphylocoagulase Genotype XV

Staphylococcus argenteus and Staphylococcus schweitzeri are cytotoxic to human cells in vitro due to high expression of alpha-hemolysin Hla

Staphylococcus argenteus as an etiological agent of prosthetic hip joint infection: a case presentation

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